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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4℃,避光保存 6 个月
- 保质期:
4℃,避光保存 6 个月
- 英文名:
Micro Trehalose Concent Assay Kit
- 库存:
999
- 供应商:
亚科因(Abbkine)
- 规格:
48 T/48 S/96 T/96 S
| 规格: | 48 T/48 S | 产品价格: | ¥406.0 |
|---|---|---|---|
| 规格: | 96 T/96 S | 产品价格: | ¥669.0 |
CheKine™ 微量化海藻糖含量检测试剂盒(比色法)
货号:KTB1335-E
产品类型:细胞代谢检测试剂盒
检测指标:海藻糖(Trehalose)
适用样本:动植物组织、细胞、细菌、血清(浆)及其他液体样本
产品概述
CheKine™ Micro Trehalose Concentration Assay Kit 是一款专为微量样本设计的海藻糖含量检测试剂盒,采用经典的蒽酮比色法,具备高灵敏度与操作简便性,适用于多种生物样本中海藻糖的定量分析。
主要特点与优势
- 广泛验证的样本类型:适用于动物、植物、微生物、培养细胞及多种液体样本;
- 微量检测:适合样本量有限的研究场景;
- 操作简便:流程清晰,无需复杂设备,降低操作门槛;
- 高灵敏度:蒽酮法具有良好的检测灵敏度与稳定性。
试剂盒组分
- Extraction Buffer
- Reagent I
- Standard
检测方法
比色法(蒽酮法),在特定波长下测定吸光度,计算海藻糖含量。
注意事项
- 推荐使用新鲜样本,若需保存,请置于 -80℃ 环境,避免反复冻融;
- 解冻应在室温下快速完成(建议不超过4小时),以保证样本稳定性;
- 实验前请确保所有试剂及设备处于适宜温度;
- 注意:本试剂盒适用于不含其他可溶性糖的样本,否则可能干扰测定结果。
保存与运输
- 保存条件:4℃避光保存,有效期6个月;
- 运输方式:蓝冰冰袋运输,确保试剂活性。
规格与定价
| 规格 | 价格(元) | 货期 |
|---|---|---|
| 48 T/48 S | 406 | 现货(次日发货) |
| 96 T/96 S | 669 | 现货(次日发货) |
背景信息
海藻糖是一种广泛存在于生物体中的非还原性双糖,在极端环境下(如干旱、高温、冷冻等)具有保护生物大分子结构稳定的功能。本试剂盒通过蒽酮比色法实现对海藻糖的特异性定量,为细胞代谢、抗逆机制等研究提供可靠工具。
警告:本品仅供科研使用,不适用于临床诊断或人体使用。使用者应负责确保其应用不违反相关专利或许可协议。
注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。

CheKine™ Micro Trehalose Assay Kit (Colorimetric Method)
Catalog Number: KTB1335-E
Product Type: Cell Metabolism Assay Kit
Detection Target: Trehalose
Suitable Samples: Animal and plant tissues, cells, bacteria, serum (plasma), and other liquid samples
Product Overview
The CheKine™ Micro Trehalose Assay Kit is specifically designed for the quantification of trehalose in limited sample volumes using the classic anthrone colorimetric method. It offers high sensitivity and ease of operation, making it suitable for quantitative analysis of trehalose in various biological samples.
Key Features and Advantages
- Broadly Validated Sample Types: Suitable for animal, plant, microbial, cultured cell, and various liquid samples;
- Micro-Scale Assay: Ideal for research scenarios with limited sample availability;
- Simple Operation: Clear procedures with no need for complex equipment, reducing operational barriers;
- High Sensitivity: The anthrone method provides excellent detection sensitivity and stability.
Kit Components
- Extraction Buffer
- Reagent I
- Standard
Detection Method
Colorimetric Method (Anthrone Method), measure absorbance at a specific wavelength to calculate trehalose content.
Precautions
- Fresh samples are recommended. If storage is necessary, keep at -80°C and avoid repeated freeze-thaw cycles;
- Thawing should be performed rapidly at room temperature (recommended not exceeding 4 hours) to ensure sample stability;
- Ensure all reagents and equipment are at appropriate temperatures before starting the experiment;
- Note: This kit is suitable for samples without other soluble sugars, as their presence may interfere with the assay results.
Storage and Shipping
- Storage Conditions: Store at 4°C protected from light; valid for 6 months;
- Shipping Method: Shipped with blue ice packs to ensure reagent activity.
Specifications and Pricing
| Specification | Price (¥) | Availability |
|---|---|---|
| 48 T/48 S | 406 | In stock (shipped next day) |
| 96 T/96 S | 669 | In stock (shipped next day) |
Background Information
Trehalose is a non-reducing disaccharide widely present in living organisms. It plays a protective role in stabilizing macromolecular structures under extreme conditions such as drought, high temperature, and freezing. This kit enables specific quantification of trehalose via the anthrone colorimetric method, providing a reliable tool for research in cell metabolism and stress resistance mechanisms.
Warning: This product is for research use only. Not intended for clinical diagnosis or human use. The user is responsible for ensuring that its application does not infringe relevant patents or licensing agreements.
Note: The above information is subject to change due to product optimization and upgrades. For specific details, please consult with the company.
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文献和实验1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
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