Rubisco试剂盒，用于样本中二磷酸核酮糖羧化酶/加氧酶检

CheKine™ 二磷酸核酮糖羧化酶/加氧酶（Rubisco）活性检测试剂盒（比色法）

货号：KTB1480-E

产品类型：细胞代谢检测试剂盒

样本类型：植物组织样本

检测方法：比色法

产品概述

CheKine™ Rubisco活性检测试剂盒是一种用于高效、准确测定植物组织样本中1,5-二磷酸核酮糖羧化酶/加氧酶（Rubisco, EC 4.1.1.39）活性的比色法检测工具。Rubisco是光合作用碳固定过程中的核心酶，其活性直接影响植物的光合效率与碳代谢流。本试剂盒提供了完整的缓冲体系与反应试剂，适用于各类植物样本的Rubisco酶活性科研分析。

检测原理

试剂盒通过比色法检测Rubisco催化1,5-二磷酸核酮糖（RuBP）羧化反应过程中NADH的氧化速率，从而间接反映酶活性。该方法灵敏度高，操作流程明确，结果稳定可靠。

试剂盒组分

• Extraction Buffer Ⅰ
• Extraction Buffer Ⅱ
• Reagent Ⅰ
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ

特点与优势

• 特异性检测植物样本中Rubisco羧化酶与加氧酶活性；
• 提供详尽的样本前处理与活性计算公式，助力数据准确性；
• 操作流程清晰，兼容常见的紫外可见分光光度计或酶标仪；
• 适用于多种植物组织，如叶片、藻类等光合器官。

规格与定价

规格	价格（元）	货期
48 T/48 S	3289	现货（次日发货）
96 T/96 S	4589	现货（次日发货）

保存与运输条件

试剂盒应在收到后避光保存于-20℃。请参考说明书了解各组分的具体存储要求。自发货日起，在适当保存条件下有效期为6个月。

运输条件：冰袋（蓝冰）运输。

注意事项

• 请勿混合使用不同批次或不同厂家的试剂组分；
• 复溶或混合试剂时应避免产生气泡；
• 操作过程中应勤换吸头，防止交叉污染；
• 实验前请确保所有试剂及设备处于合适温度。

警告

本产品仅用于科研研究，不适用于人类或临床诊断。使用本产品应遵守相关法律法规，并注意可能涉及的专利许可问题。厂家不对因使用本产品所导致的专利侵权或其他违规行为承担责任。

别名

二磷酸核酮糖羧化酶；Rubisco

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Ribulose-1,5-bisphosphate Carboxylase/Oxygenase (Rubisco) Activity Assay Kit (Colorimetric Method)

Catalog Number: KTB1480-E

Product Type: Cell Metabolism Assay Kit

Sample Type: Plant Tissue Samples

Assay Method: Colorimetric Method

Product Overview

The CheKine™ Rubisco Activity Assay Kit provides a high-efficiency and accurate colorimetric method for the determination of Ribulose-1,5-bisphosphate Carboxylase/Oxygenase (Rubisco, EC 4.1.1.39) activity in plant tissue samples. Rubisco is the key enzyme in the carbon fixation process of photosynthesis, and its activity directly affects photosynthetic efficiency and carbon metabolic flux. This kit includes a complete buffer system and reaction reagents, making it suitable for Rubisco enzyme activity research analysis in various plant samples.

Assay Principle

The kit employs a colorimetric method to measure the oxidation rate of NADH during the carboxylation reaction of Ribulose-1,5-bisphosphate (RuBP) catalyzed by Rubisco, thereby indirectly reflecting enzymatic activity. This method offers high sensitivity, a clear operational procedure, and stable, reliable results.

Kit Components

• Extraction Buffer Ⅰ
• Extraction Buffer Ⅱ
• Reagent Ⅰ
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ

Features & Advantages

• Specifically detects both carboxylase and oxygenase activities of Rubisco in plant samples;
• Provides detailed sample pretreatment protocols and activity calculation formulas to ensure data accuracy;
• Clear operational procedure, compatible with common UV-Vis spectrophotometers or microplate readers;
• Suitable for various plant tissues, such as leaves, algae, and other photosynthetic organs.

Specifications & Pricing

Specification	Price (RMB)	Availability
48 T/48 S	3289	In Stock (Ships Next Day)
96 T/96 S	4589	In Stock (Ships Next Day)

Storage & Shipping Conditions

Upon receipt, store the kit protected from light at -20℃. Refer to the manual for specific storage requirements of individual components. The kit is stable for 6 months from the date of shipment when stored properly.

Shipping Conditions: Shipped with ice packs (blue ice).

Precautions

• Do not mix reagents from different batches or manufacturers;
• Avoid bubble formation during reagent reconstitution or mixing;
• Change pipette tips frequently to prevent cross-contamination;
• Ensure all reagents and equipment are at the appropriate temperature before starting the experiment.

Warning

This product is intended for research use only, not for human or clinical diagnostic use. Use of this product should comply with relevant laws and regulations, and attention should be paid to potential patent licensing issues. The manufacturer is not liable for patent infringement or other violations resulting from the use of this product.

Aliases

Ribulose-1,5-bisphosphate carboxylase; Rubisco

Note: The above information is subject to change due to product optimization and upgrades. For detailed information, please consult with YaKenin Company.