乙酰胆碱酯酶活性检测试剂盒，用于对样本中AchE活性检测，A

CheKine™ 微量乙酰胆碱酯酶（AchE）活性检测试剂盒

货号：KTB1710-C

产品概述

CheKine™ 乙酰胆碱酯酶（AchE）活性检测试剂盒（微量法）是一款基于比色法原理开发的检测工具，可精准、高效地测定多种生物样本中的乙酰胆碱酯酶活性。该试剂盒适用于新鲜血清（浆）、动物组织及神经细胞样本，广泛应用于神经科学、毒理学及细胞代谢研究领域。

检测原理

乙酰胆碱酯酶（AchE）属于丝氨酸水解酶家族，在神经信号传导过程中起关键作用，负责催化神经递质乙酰胆碱（Ach）水解。本试剂盒利用AchE催化Ach水解生成胆碱，胆碱进一步与显色剂二硫对硝`基`苯甲酸（DTNB）反应，生成在412 nm处具有特征吸收峰的黄色化合物5-巯基-硝`基`苯甲酸（TNB）。通过动态监测412 nm波长下吸光值的上升速率，即可准确计算出AchE的酶活性水平。

产品特点与优势

• 专为微量检测设计，适用于多种样本类型，包括动物组织、血清（浆）及体外培养细胞；
• 提供完整的系统解决方案，含详细样本前处理指南与酶活性计算公式；
• 操作流程清晰，试剂稳定性良好，实验结果重复性高；
• 不含人体来源成分，不适用于临床诊断。

试剂盒组分

• Extraction Buffer
• Assay Buffer
• Chromogen（显色剂）
• Substrate（底物）

样本处理建议

推荐使用新鲜样本进行检测。如不能立即检测，样本可于-80℃保存数周。避免反复冻融，解冻过程应在低温环境下控制时间，如在室温解冻需在4小时内完成实验。

保存与运输

本品需4℃避光保存，有效期为12个月。运输全程采用蓝冰冰袋保护，确保试剂活性。

注意事项

• 本产品仅用于科学研究，不得用于人体或临床诊断；
• 实验前请确保所有试剂及设备处于适宜温度；
• 使用者应自行确保应用方式不侵犯任何专利权或许可协议。

规格与价格

规格：96 T/96 S（96次检测）

价格：¥398

货期：现货，次日发货

如需了解更多信息或技术支持，请联系我们获取产品说明书及资料。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Micro Acetylcholinesterase (AChE) Activity Assay Kit

Catalog Number:KTB1710-C

Product Overview

The CheKine™ Acetylcholinesterase (AChE) Activity Assay Kit (Micro Method) is a colorimetric-based assay tool designed for the accurate and efficient determination of acetylcholinesterase activity in various biological samples. This kit is suitable for fresh serum (plasma), animal tissues, and neural cell samples, and is widely used in neuroscience, toxicology, and cellular metabolism research.

Assay Principle

Acetylcholinesterase (AChE), a member of the serine hydrolase family, plays a critical role in neural signal transduction by catalyzing the hydrolysis of the neurotransmitter acetylcholine (ACh). This kit utilizes the hydrolysis of ACh by AChE to generate choline, which subsequently reacts with the chromogenic agent 5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB) to produce the yellow compound 5-thio-2-nitrobenzoic acid (TNB), which exhibits a characteristic absorption peak at 412 nm. By dynamically monitoring the rate of increase in absorbance at 412 nm, the enzymatic activity of AChE can be accurately calculated.

Features and Advantages

• Designed for micro-detection, suitable for various sample types including animal tissues, serum (plasma), and in vitro cultured cells;
• Provides a complete system solution, including detailed sample preparation guidelines and enzyme activity calculation formulas;
• Clear operating procedure, excellent reagent stability, and high experimental reproducibility;
• Contains no human-derived components and is not intended for clinical diagnosis.

Kit Components

• Extraction Buffer
• Assay Buffer
• Chromogen
• Substrate

Sample Handling Recommendations

Fresh samples are recommended for assay. If immediate testing is not possible, samples can be stored at -80°C for several weeks. Avoid repeated freeze-thaw cycles. Thawing should be controlled at low temperature; if thawed at room temperature, complete the experiment within 4 hours.

Storage and Shipping

This product must be stored protected from light at 4°C and has a shelf life of 12 months. It is shipped with blue ice packs to ensure reagent activity throughout transit.

Precautions

• This product is for research use only and is not intended for human use or clinical diagnosis;
• Ensure all reagents and equipment are at appropriate temperatures before starting the experiment;
• The user is responsible for ensuring that the application does not infringe any patents or license agreements.

Specifications and Price

Size:96 T/96 S (96 tests)

Price:¥398

Availability:In stock, ships next day

For more information or technical support, please contact us for the product manual and latest materials.

Note: The above information is subject to change due to product optimization and upgrades. Please consult APTAC for specific details.