SS-I试剂盒，用于样本中蔗糖合成酶活性检测，SS-I Ac

CheKine™ 微蔗糖合成酶-I（SS-I）活性检测试剂盒

货号：KTB3140-E

产品分类：细胞分析核心产品 | 应用领域：细胞代谢研究

产品概述

CheKine™ Micro Sucrose Synthase-I (SS-I) Activity Assay Kit是一款基于比色法原理、专业用于检测植物组织样本中蔗糖合成酶-I（SS-I）活性的试剂盒。该试剂盒通过精确测定酶促反应生成的还原糖含量，为研究植物蔗糖降解与淀粉合成过程提供可靠、高效的检测手段。

背景信息

蔗糖合成酶（Sucrose Synthase, SS-I）是调节植物体内蔗糖代谢的关键酶，其分解方向活性直接影响蔗糖向果糖和UDPG的转化过程，进而影响“库”器官中淀粉的积累与能量供应。研究SS-I活性对理解植物碳分配和生长发育机制具有重要意义。

检测原理

本试剂盒基于酶促反应与化学显色相结合的原理：SS-I催化蔗糖与UDP反应生成游离果糖和UDPG，进一步通过3,5-二硝基水杨酸（DNS）法测定还原糖含量，从而准确反映SS-I酶活性水平。

试剂盒组成

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ
• Standard

特点与优势

• 验证样本类型广泛，适用于多种植物组织
• 操作流程简便，反应体系优化，适合高通量检测
• 灵敏度高，重复性好，结果稳定可靠

适用样本

适用于植物组织样本，建议使用新鲜样本进行检测。若需保存，请于-80℃条件下冻存，并在解冻后4小时内完成实验。

保存与运输

保存条件：-20℃避光保存，有效期6个月。
运输方式：冰袋（蓝冰）运输，保障试剂稳定性。

规格与价格

规格	价格（元）	货期
48 T/24 S	798	现货（次日发货）
96 T/48 S	1326	现货（次日发货）

注意事项

• 推荐使用新鲜样本，若需保存请置于-80℃，避免反复冻融
• 实验前请确保所有试剂及设备处于适宜温度
• 解冻后的样本应在4小时内完成检测

警告：本产品仅用于科研研究，不适用于人类或临床诊断。使用者应负责确保应用方式不侵犯任何专利或许可协议。

别名：蔗糖合成酶（SS-I）；Sucrose Synthase-I Activity Assay Kit

相关分子：FBP（果糖-1,6-二磷酸）

检测指标：FBP含量间接反映SS-I酶活性

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Micro Sucrose Synthase-I (SS-I) Activity Assay Kit

Catalog Number: KTB3140-E

Product Category: Cell Analysis Core Products | Research Area: Cellular Metabolism Studies

Product Overview

The CheKine™ Micro Sucrose Synthase-I (SS-I) Activity Assay Kit is a colorimetric-based kit designed for the specific detection of Sucrose Synthase-I (SS-I) activity in plant tissue samples. By accurately quantifying the amount of reducing sugars generated via enzymatic reaction, this kit provides a reliable and efficient tool for investigating sucrose degradation and starch biosynthesis pathways in plants.

Background

Sucrose Synthase (SS-I) is a key enzyme regulating sucrose metabolism in plants. Its degradative activity directly influences the conversion of sucrose into fructose and UDP-glucose, thereby affecting starch accumulation and energy supply in "sink" organs. Studying SS-I activity is crucial for understanding carbon allocation and growth mechanisms in plants.

Assay Principle

This kit employs a combination of enzymatic reaction and chromogenic detection: SS-I catalyzes the reaction of sucrose and UDP to produce free fructose and UDPG. The resulting reducing sugars are quantified using the 3,5-dinitrosalicylic acid (DNS) method, providing an accurate measure of SS-I enzymatic activity.

Kit Components

• Extraction Buffer
• Reagent I
• Reagent II
• Reagent III
• Reagent IV
• Standard

Features & Advantages

• Broad sample compatibility, suitable for various plant tissues
• Streamlined protocol with optimized reaction system, ideal for high-throughput assays
• High sensitivity, excellent reproducibility, and reliable results

Applicable Samples

Suitable for plant tissue samples. Fresh samples are recommended. If storage is necessary, freeze at -80°C and complete the assay within 4 hours after thawing.

Storage & Shipping

Storage Conditions: Store at -20°C protected from light; stable for 6 months.
Shipping: Shipped with ice packs (blue ice) to ensure reagent stability.

Specifications & Pricing

Specification	Price (¥)	Availability
48 T/24 S	798	In Stock (Ships Next Day)
96 T/48 S	1326	In Stock (Ships Next Day)

Notes

• Fresh samples are recommended. If stored, keep at -80°C and avoid repeated freeze-thaw cycles
• Ensure all reagents and equipment are at appropriate temperatures before starting the assay
• Assay should be completed within 4 hours after sample thawing

Warning: This product is for research use only. Not intended for human or clinical diagnostic use. The user is responsible for ensuring that application does not infringe any patents or licensing agreements.

Alias: Sucrose Synthase (SS-I); Sucrose Synthase-I Activity Assay Kit

Related Molecule: FBP (Fructose-1,6-bisphosphate)

Assay Target: FBP content indirectly reflects SS-I enzymatic activity

Note: The above information is subject to change due to product optimization and updates. For detailed information, please contact Yackein Biosciences.