谷氨酸合成酶活性检测试剂盒，用于对样本中GOGAT活性检测，

谷氨酸合成酶（GOGAT）活性检测试剂盒

货号：KTB3040-C

产品类型：细胞代谢检测试剂盒

样本类型：适用于微生物、植物非绿色组织等多种样本来源

产品概述

本试剂盒是一款基于比色法原理，专业用于定量检测样本中谷氨酸合成酶（GOGAT）活性的研究工具。GOGAT是氨同化过程中的关键酶之一，广泛存在于原核生物、酵母及高等植物的非绿色组织中。通过准确测定GOGAT酶活，可为细胞代谢研究，尤其是氮代谢与氨基酸合成途径提供可靠数据支持。

检测原理

试剂盒采用特异性底物与反应体系，在NADH参与下，GOGAT催化谷氨酰胺和α-酮戊二酸生成谷氨酸，通过检测340 nm处吸光度的下降速率，即可计算出GOGAT酶活性。

试剂盒组分

• ReagentⅠ
• ReagentⅡ
• ReagentⅢ

重要参数

• 检测方法：比色法（UV微孔板法）
• 规格选项：
  • 48 T/48 S — ¥539（现货，次日发货）
  • 96 T/96 S — ¥898（现货，次日发货）
• 运输条件：蓝冰冰袋运输
• 保存建议：4℃避光保存，有效期12个月（自发货日起）

注意事项

• 需使用96孔UV微孔板进行检测。
• 严禁混用不同批次或不同厂家的试剂组分。
• 样本处理需在冰上操作，并于当日完成测定，避免匀浆液反复冻融。
• 实验前请确保所有试剂及设备处于适宜温度。

应用领域

• 微生物氮代谢研究
• 植物非绿色组织氨基酸代谢分析
• 细胞代谢通路与酶功能研究

警示：本产品仅用于科研用途，不适用于人类或临床诊断。使用者应确保应用行为不侵犯任何专利或许可权利，本公司不对专利侵权或其他违规行为承担责任。

别名：谷氨酸合成酶, GOGAT, Glutamate Synthase

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Glutamate Synthase (GOGAT) Activity Assay Kit

Catalog Number: KTB3040-C

Product Type: Cell Metabolism Assay Kit

Sample Types: Suitable for a variety of sample sources including microorganisms and non-green plant tissues

Product Overview

This kit is a research tool specifically designed for the quantitative determination of Glutamate Synthase (GOGAT) activity in samples, based on the colorimetric method. GOGAT is one of the key enzymes in the ammonia assimilation process, widely present in prokaryotes, yeasts, and non-green tissues of higher plants. Accurate measurement of GOGAT enzyme activity provides reliable data support for research in cellular metabolism, particularly in nitrogen metabolism and amino acid synthesis pathways.

Assay Principle

The kit employs specific substrates and a reaction system. With NADH participation, GOGAT catalyzes the conversion of glutamine and α-ketoglutarate to glutamate. The rate of decrease in absorbance at 340 nm is measured to calculate GOGAT enzyme activity.

Kit Components

• ReagentⅠ
• ReagentⅡ
• ReagentⅢ

Key Parameters

• Assay Method: Colorimetric method (UV microplate method)
• Size Options:
  • 48 T/48 S — ¥539 (In stock, ships next day)
  • 96 T/96 S — ¥898 (In stock, ships next day)
• Shipping Conditions: Shipped with blue ice packs
• Storage Recommendation: Store protected from light at 4°C; stable for 12 months (from the date of shipment)

Precautions

• Requires the use of a 96-well UV microplate for detection.
• Do not mix reagents from different batches or different manufacturers.
• Sample processing must be performed on ice, and assays should be completed on the same day. Avoid repeated freeze-thaw cycles of homogenates.
• Ensure all reagents and equipment are at appropriate temperatures before starting the experiment.

Application Fields

• Research on microbial nitrogen metabolism
• Analysis of amino acid metabolism in non-green plant tissues
• Studies on cellular metabolic pathways and enzyme function

Warning: This product is for research use only. It is not intended for human or clinical diagnostic use. The user is responsible for ensuring that their application does not infringe any patents or license rights. The company assumes no liability for patent infringement or other violations.

Aliases: Glutamate Synthase, GOGAT

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult with Yacoin Company.