脯氨酸试剂盒，用于样本中PRO含量检测，微量法，PRO As

Micro Proline (PRO) Assay Kit | 脯氨酸试剂盒（微量法）

货号：KTB1430-S

产品分类：核心产品 · 细胞代谢检测

产品概述

Micro Proline (PRO) Assay Kit 是一款基于比色法原理的高灵敏度检测试剂盒，用于定量测定多种生物样本中的脯氨酸（PRO）含量。该试剂盒采用优化的微量检测体系，适用于植物组织、动物组织、细胞、细菌以及血清（浆）等多种样品类型，检测范围广泛，操作流程清晰，配套标准品与标准曲线，为科研实验提供可靠的数据支持。

主要特点与优势

• 广泛样本适用性：适用于植物、动物组织、细胞、细菌、血清（浆）等样品的PRO含量分析。
• 高灵敏度与宽线性范围：检测灵敏度达0.5 μg/mL，线性范围为0.5–80 μg/mL，满足多数实验需求。
• 标准品与标准曲线提供：内含PRO标准品，协助用户构建标准曲线，提升结果准确性。
• 详尽的实验指南：提供清晰的样品制备步骤与结果计算方法，降低操作难度。

检测参数

• 检测方法：比色法
• 检测类型：氨基酸代谢
• 检测指标：脯氨酸（PRO）
• 灵敏度：0.5 μg/mL
• 检测范围：0.5–80 μg/mL
• 所需设备：96孔UV微孔板

试剂盒组分

• Extraction Buffer
• Assay Buffer I
• Chromogen
• Pro标准品

规格与价格

规格	价格（元）	货期
48 T/48 S	726	现货（当天发货）
96 T/96 S	1098	现货（当天发货）

背景与应用

脯氨酸（PRO，C₅H₉NO₂）广泛存在于动植物、微生物及培养细胞中，在逆境条件下（如干旱、盐胁迫等），植物体内脯氨酸含量常显著升高，其积累程度与抗逆性密切相关，可作为抗逆育种的重要生理指标之一。本试剂盒为相关领域研究提供了一种稳定、高效的检测工具。

注意事项

• 实验需使用96孔UV微孔板。
• 避免不同批次或不同厂家的试剂组分混合使用。
• 操作过程中请勤换吸头，防止交叉污染。
• 混合或复溶试剂时应避免产生气泡。
• 实验前请确保所有组分及设备处于合适温度。

保存与运输

保存条件：收到后请避光保存于4℃。自发货日起，在推荐保存条件下有效期为6个月。

运输条件：冰袋（蓝冰）运输。

警告

本品仅供科研使用，不适用于人类或临床诊断。使用本品不得违反任何专利或许可规定，厂商对因使用本品导致的专利侵权或其他违规行为不承担任何责任。

别名：C5H9NO2, Pyrrolidine-2-carboxylic acid, D-吡`咯`烷-2-羧酸, 脯氨酸, PRO, Proline

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Micro Proline (PRO) Assay Kit | Proline Assay Kit (Microscale Method)

Catalog Number: KTB1430-S

Product Category: Core Products · Cell Metabolism Assays

Product Overview

The Micro Proline (PRO) Assay Kit is a highly sensitive colorimetric-based detection kit designed for the quantitative determination of proline (PRO) content in a variety of biological samples. This kit employs an optimized microscale detection system suitable for multiple sample types including plant tissues, animal tissues, cells, bacteria, and serum (plasma). It offers a broad detection range, a clear operational procedure, and includes standards and a standard curve to provide reliable data support for scientific research.

Key Features and Advantages

• Broad Sample Applicability: Suitable for proline content analysis in samples such as plants, animal tissues, cells, bacteria, and serum (plasma).
• High Sensitivity and Wide Linear Range: Detection sensitivity reaches 0.5 μg/mL, with a linear range of 0.5–80 μg/mL, meeting the needs of most experiments.
• Provided Standards and Standard Curve: Includes PRO standards to assist users in constructing a standard curve, enhancing result accuracy.
• Comprehensive Experimental Guide: Provides clear sample preparation steps and result calculation methods to reduce operational difficulty.

Detection Parameters

• Detection Method: Colorimetric method
• Detection Type: Amino acid metabolism
• Detection Target: Proline (PRO)
• Sensitivity: 0.5 μg/mL
• Detection Range: 0.5–80 μg/mL
• Required Equipment: 96-well UV microplate

Kit Components

• Extraction Buffer
• Assay Buffer I
• Chromogen
• Pro Standard

Specifications and Pricing

Specification	Price (¥)	Lead Time
48 T/48 S	726	In Stock (Ships Same Day)
96 T/96 S	1098	In Stock (Ships Same Day)

Background and Applications

Proline (PRO, C₅H₉NO₂) is widely present in plants, animals, microorganisms, and cultured cells. Under stress conditions (such as drought, salt stress, etc.), proline content in plants often increases significantly, and its accumulation level is closely related to stress resistance, making it an important physiological indicator for stress-resistant breeding. This kit provides a stable and efficient detection tool for research in related fields.

Precautions

• Experiments require the use of a 96-well UV microplate.
• Avoid mixing reagents from different batches or manufacturers.
• Change pipette tips frequently during operation to prevent cross-contamination.
• Avoid generating bubbles when mixing or reconstituting reagents.
• Ensure all components and equipment are at appropriate temperatures before starting the experiment.

Storage and Shipping

Storage Conditions: Upon receipt, store protected from light at 4°C. The kit is stable for 6 months from the date of shipment when stored under recommended conditions.

Shipping Conditions: Shipped with ice packs (blue ice).

Warning

This product is intended for research use only and is not suitable for human or clinical diagnostic use. Use of this product must not infringe any patent or license regulations. The manufacturer assumes no liability for patent infringement or other violations resulting from the use of this product.

Aliases: C5H9NO2, Pyrrolidine-2-carboxylic acid, D-Pyrrolidine-2-carboxylic acid, Proline, PRO

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yacoin Company.