ICDHm试剂盒，用于样本中线粒体异柠檬酸脱氢酶活性检测，I

ICDHm试剂盒：线粒体异柠檬酸脱氢酶活性检测解决方案

产品概述

本产品为Micro Mitochondrial Isocitrate Dehydrogenase (ICDHm) Activity Assay Kit（货号：KTB1250-E），是一款基于比色法原理的专业检测试剂盒，专注于线粒体异柠檬酸脱氢酶（ICDHm）活性的精准测定。该酶广泛存在于动物、植物、微生物及培养细胞的线粒体中，是三羧酸循环中的关键酶之一，催化异柠檬酸脱羧生成α-酮戊二酸，并伴随NADP+还原为NADPH的过程。

主要特点与优势

• 提供简单、快速、高兼容性的检测方案，适用于多种样本类型（动物组织、植物组织、细胞、真菌等）；
• 试剂盒内含完整组分，包括Extraction Buffer及五种专用试剂（ReagentⅠ至Ⅴ），配套详细的样品制备与结果计算指南；
• 支持灵活规格选择：48T/48S与96T/96S两种配置，满足不同通量需求。

检测原理与方法

本试剂盒采用比色法，通过测定单位时间内NADPH的生成速率，间接反映ICDHm的酶活性。该方法灵敏度高、操作简便，适用于大多数实验室条件下的酶动力学研究。

适用样本与检测范围

兼容多种生物样本，包括但不限于：

• 动物组织（如肝脏、肌肉等）；
• 植物组织；
• 细胞提取物；
• 真菌样本。

注意事项

• 测定管ΔA值应控制在0.01–0.3之间，超出范围需调整样本稀释倍数；
• 严禁混用不同批次或不同厂家的试剂组分；
• 实验全程需在低温环境下进行，避免酶失活；
• 建议使用新鲜样本提取ICDHm，以保证酶活力稳定性；
• 推荐以样本蛋白浓度计算酶活，若以样本质量计算需额外测定胞浆提取物酶活。

保存与运输

• 保存条件：有效期6个月，具体组分储存条件请参考包装清单；
• 运输方式：冰袋（蓝冰）运输，确保试剂活性。

规格与价格

警告：本产品仅供研究使用，不适用于人类或临床诊断。使用者应自行确保应用场景不违反相关专利或许可协议。

如需了解更多技术细节或应用支持，请联系我们的专业技术团队。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

ICDHm Assay Kit: A Solution for Mitochondrial Isocitrate Dehydrogenase Activity Detection

Product Overview

This product is the Micro Mitochondrial Isocitrate Dehydrogenase (ICDHm) Activity Assay Kit (Cat. No.: KTB1250-E), a professional assay kit based on the colorimetric principle, specifically designed for the accurate determination of mitochondrial isocitrate dehydrogenase (ICDHm) activity. This enzyme is widely present in the mitochondria of animals, plants, microorganisms, and cultured cells. It is a key enzyme in the tricarboxylic acid (TCA) cycle, catalyzing the decarboxylation of isocitrate to α-ketoglutarate coupled with the reduction of NADP+ to NADPH.

Key Features and Advantages

• Provides a simple, rapid, and highly compatible detection solution suitable for various sample types (animal tissues, plant tissues, cells, fungi, etc.);
• The kit contains complete components, including Extraction Buffer and five specialized reagents (Reagent I to V), accompanied by detailed sample preparation and result calculation guidelines;
• Supports flexible size options: 48T/48S and 96T/96S configurations to meet different throughput needs.

Assay Principle and Method

This kit employs a colorimetric method to indirectly reflect ICDHm enzyme activity by measuring the rate of NADPH generation per unit time. This method is highly sensitive, operationally simple, and suitable for enzyme kinetic studies under most laboratory conditions.

Compatible Samples and Detection Range

Compatible with a variety of biological samples, including but not limited to:

• Animal tissues (e.g., liver, muscle);
• Plant tissues;
• Cellular extracts;
• Fungal samples.

Precautions

• The ΔA value of the assay tube should be controlled within the range of 0.01–0.3. If outside this range, adjust the sample dilution factor;
• Do not mix reagents from different batches or manufacturers;
• The entire experiment must be conducted under low-temperature conditions to prevent enzyme denaturation;
• It is recommended to use fresh samples for ICDHm extraction to ensure enzyme activity stability;
• Enzyme activity is recommended to be calculated based on sample protein concentration. If calculated based on sample weight, additional measurement of cytosolic extract enzyme activity is required.

Storage and Shipping

• Storage Conditions: Valid for 6 months. Please refer to the packaging list for specific component storage conditions;
• Shipping Method: Shipped with ice packs (blue ice) to ensure reagent activity.

Specifications and Pricing

Warning: This product is for research use only. Not intended for human use or clinical diagnosis. Users are responsible for ensuring that their application does not infringe relevant patents or licensing agreements.

For more technical details or application support, please contact our professional technical team.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult ACROBIO Systems Co.