Total sugar试剂盒，用于样本中总糖含量检测，Tot

Micro Total Carbohydrate Assay Kit (总糖含量检测试剂盒)

货号：KTB1350-E

产品分类：核心产品｜细胞代谢检测

产品概述

Micro Total Carbohydrate Assay Kit 是一款基于比色法原理、专业用于检测各类生物样本中总糖含量的高灵敏试剂盒。该试剂盒适用于包括动物组织、植物组织、血清及血浆在内的多种样本类型，提供稳定、可靠的检测结果，是细胞代谢研究中的重要工具。

检测原理与应用范围

本试剂盒通过酸水解将样本中的多糖与寡糖分解为还原性单糖，再利用DNS试剂与还原糖发生显色反应，在特定波长下测定吸光度，从而准确计算出总糖含量。总糖主要包括葡萄糖、果糖、乳糖等还原糖，以及蔗糖、麦芽糖和可部分水解的淀粉。

主要应用领域：细胞糖代谢研究、食品科学、植物生理学及生物能源开发等。

试剂盒组分

• Hydrochloric Acid（盐酸）
• Sodium Hydroxide（氢`氧`化`钠）
• DNS Reagent（DNS显色试剂）
• Standard（标准品）

特点与优势

• 操作简便快速：优化的流程设计显著缩短检测时间；
• 高灵敏度：最低检测浓度可达 0.1 mg/mL；
• 广泛的样本兼容性：支持动物/植物组织及血清血浆样品；
• 提供完整方案：含详尽的样本前处理步骤与结果计算指南。

技术参数

• 检测范围：0.1 - 2 mg/mL
• 灵敏度：0.1 mg/mL
• 检测方法：比色法（DNS法）
• 样本类型：组织匀浆、血清、血浆及细胞提取液等

规格与价格

规格	价格（元）	货期
48 T	458	现货，次日发货
96 T	716	现货，次日发货

使用注意事项

• 严禁混用不同批次或不同厂家的试剂组分；
• 操作 Hydrochloric Acid 与 Sodium Hydroxide 时需穿戴防护用具，避免接触皮肤；
• DNS Reagent 具有一定毒性，实验应在通风条件下进行；
• 建议进行预实验以确定样本最佳稀释倍数；
• 注意避免气泡产生和交叉污染；
• 本试剂盒对纤维素的水解效率有限，不完全适用于高纤维素样本。

保存与运输条件

试剂盒需保存于 4℃ 环境下，有效期为12个月。运输过程中采用蓝冰冰袋保护，以保证试剂稳定性。

警告：本产品仅限科研使用，不适用于人类或临床诊断。使用者应负责确保其应用不侵犯任何专利权或许可证。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Micro Total Carbohydrate Assay Kit

Catalog Number: KTB1350-E

Product Category: Core Products｜Cell Metabolism Assays

Product Overview

The Micro Total Carbohydrate Assay Kit is a highly sensitive colorimetric-based kit specifically designed for the detection of total carbohydrate content in various biological samples. It is suitable for multiple sample types including animal tissues, plant tissues, serum, and plasma, providing stable and reliable results, making it an essential tool in cellular metabolism research.

Principle and Applications

This kit employs acid hydrolysis to break down polysaccharides and oligosaccharides in samples into reducing monosaccharides. The resulting reducing sugars then react with DNS reagent to produce a colorimetric signal, which is measured at a specific wavelength to accurately calculate total carbohydrate content. Total carbohydrates include reducing sugars such as glucose, fructose, and lactose, as well as sucrose, maltose, and partially hydrolysable starch.

Major Application Areas: Research on cellular sugar metabolism, food science, plant physiology, and bioenergy development.

Kit Components

• Hydrochloric Acid
• Sodium Hydroxide
• DNS Reagent
• Standard

Features and Advantages

• Simple and Rapid Operation: Optimized procedure significantly reduces assay time;
• High Sensitivity: Minimum detectable concentration as low as 0.1 mg/mL;
• Broad Sample Compatibility: Supports animal/plant tissues, serum, and plasma samples;
• Comprehensive Protocol: Includes detailed sample preparation steps and result calculation guidelines.

Technical Parameters

• Detection Range: 0.1 - 2 mg/mL
• Sensitivity: 0.1 mg/mL
• Assay Method: Colorimetric (DNS method)
• Sample Types: Tissue homogenates, serum, plasma, cell lysates, etc.

Specifications and Pricing

Specification	Price (¥)	Availability
48 T	458	In stock, ships next day
96 T	716	In stock, ships next day

Precautions

• Do not mix reagents from different batches or manufacturers;
• Wear protective equipment when handling Hydrochloric Acid and Sodium Hydroxide to avoid skin contact;
• DNS Reagent is toxic; perform experiments under ventilated conditions;
• Preliminary experiments are recommended to determine optimal sample dilution factors;
• Avoid bubble formation and cross-contamination;
• This kit has limited efficiency in hydrolyzing cellulose and is not fully suitable for samples with high cellulose content.

Storage and Shipping Conditions

The kit should be stored at 4°C and is stable for 12 months. It is shipped with blue ice packs to ensure reagent stability during transportation.

Warning: This product is for research use only. Not intended for human or clinical diagnostic use. The user is responsible for ensuring that their application does not infringe any patents or licenses.

Note: The above information is subject to change due to product optimization and updates. For detailed information, please consult Yacoin Company.