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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4℃,避光保存 6 个月
- 保质期:
4℃,避光保存 6 个月
- 英文名:
Micro β-1,3 Glucanase (β-1,3-GA) Activity Assay Kit
- 库存:
999
- 供应商:
亚科因(Abbkine)
- 规格:
48 T/24 S/96 T/48 S
| 规格: | 48 T/24 S | 产品价格: | ¥678.0 |
|---|---|---|---|
| 规格: | 96 T/48 S | 产品价格: | ¥1126.0 |
CheKine™ 微量β-1,3葡聚糖酶(β-1,3-GA)活性检测试剂盒
货号:KTB1325-E | 产品类型:细胞代谢检测试剂盒
产品概述
CheKine™ Micro β-1,3 Glucanase (β-1,3-GA) Activity Assay Kit是一款专业用于定量检测多种生物样本中β-1,3葡聚糖酶活性的高精度试剂盒。该试剂盒基于比色法原理,通过酶促反应生成还原糖并测定其速率,实现对β-1,3-GA活性的准确、稳定分析。广泛应用于植物病理学、逆境生理及细胞代谢研究领域。
检测原理
β-1,3葡聚糖酶可水解昆布多糖中的β-1,3-葡萄糖苷键,生成具有还原末端的糖类物质。本试剂盒通过特异性反应体系检测还原糖的生成量,进而精确计算酶活性水平。
主要特点与优势
- 广泛样本兼容性:经验证,适用于动植物组织、细菌、培养细胞、血清(浆)及其他液体样本;
- 操作流程简便:优化实验步骤,减少操作时间,降低人为误差;
- 提供完整分析方案:含详尽的样本制备指南与结果计算方法,助力数据准确性与可重复性。
试剂盒组分
- Extraction Buffer
- Reagent I
- Reagent II
- Standard
检测范围与应用
适用于定量分析动植物组织、细菌、细胞培养物及血清(浆)等样本中的β-1,3葡聚糖酶(β-1,3-GA)活性。特别推荐用于植物逆境响应及病原感染过程中的酶活性变化研究。
保存与运输条件
保存条件:4℃避光保存,有效期6个月。
运输方式:冰袋(蓝冰)低温运输,保障试剂稳定性。
注意事项
- 推荐使用新鲜样本。如不能立即检测,样本可在-80℃保存数周,避免反复冻融;
- 解冻需控制温度与时间,室温解冻应在4小时内完成;
- 实验前请确保所有试剂及设备处于适宜温度,以保证反应一致性。
规格与定价
| 规格 | 价格(元) | 货期 |
|---|---|---|
| 48 T/24 S | 678 | 现货,次日发货 |
| 96 T/48 S | 1126 | 现货,次日发货 |
警告:本产品仅用于科学研究目的,不适用于人类或临床诊断。使用者应确保应用行为不违反相关专利或许可协议,本公司不对因使用本产品导致的专利侵权或违规行为承担责任。
别名:β-1,3葡聚糖酶(β-1,3-GA);β-1,3-Glucanase
注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。

CheKine™ Micro β-1,3 Glucanase (β-1,3-GA) Activity Assay Kit
Catalog Number: KTB1325-E | Product Type: Cell Metabolism Assay Kit
Product Overview
The CheKine™ Micro β-1,3 Glucanase (β-1,3-GA) Activity Assay Kit is a high-precision kit specifically designed for the quantitative detection of β-1,3 glucanase activity in various biological samples. Based on the colorimetric principle, this kit accurately and stably analyzes β-1,3-GA activity by measuring the rate of reducing sugar generation through enzymatic reactions. It is widely used in plant pathology, stress physiology, and cell metabolism research.
Assay Principle
β-1,3 glucanase hydrolyzes the β-1,3-glycosidic bonds in laminarin, producing sugar molecules with reducing ends. This kit detects the amount of reducing sugar generated through a specific reaction system, enabling precise calculation of enzyme activity levels.
Key Features and Advantages
- Broad Sample Compatibility: Validated for use with animal and plant tissues, bacteria, cultured cells, serum (plasma), and other liquid samples;
- Simplified Workflow: Optimized experimental procedures reduce operation time and minimize human error;
- Comprehensive Analysis Solution: Includes detailed sample preparation guidelines and result calculation methods to ensure data accuracy and reproducibility.
Kit Components
- Extraction Buffer
- Reagent I
- Reagent II
- Standard
Detection Range and Applications
Suitable for quantitative analysis of β-1,3 glucanase (β-1,3-GA) activity in samples such as animal and plant tissues, bacteria, cell cultures, and serum (plasma). Particularly recommended for studying enzyme activity changes during plant stress response and pathogen infection processes.
Storage and Shipping Conditions
Storage Conditions: Store at 4°C protected from light; valid for 6 months.
Shipping Method: Shipped with ice packs (blue ice) under low temperature to ensure reagent stability.
Precautions
- Fresh samples are recommended. If not tested immediately, samples can be stored at -80°C for several weeks; avoid repeated freeze-thaw cycles;
- Thawing should be controlled for temperature and time; thawing at room temperature should be completed within 4 hours;
- Ensure all reagents and equipment are at appropriate temperatures before the experiment to maintain reaction consistency.
Specifications and Pricing
| Specification | Price (CNY) | Lead Time |
|---|---|---|
| 48 T/24 S | 678 | In stock, shipped next day |
| 96 T/48 S | 1126 | In stock, shipped next day |
Warning: This product is intended for research purposes only and is not suitable for human or clinical diagnostic use. Users should ensure that their application does not infringe any relevant patents or licensing agreements. The company is not liable for any patent infringement or non-compliance resulting from the use of this product.
Alias: β-1,3 Glucanase (β-1,3-GA); β-1,3-Glucanase
Note: The above information may change due to product optimization and upgrades. Please consult Yakeyin Company for specific details.
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文献和实验1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
Assay of Phospholipase A Activity
are important signal molecules in the cellular adaptation to stress, developmental processes and several diseases in plants and animals. The assay of PLA activity has been much promoted by the availability of phospholipid substrates with fluorophores
Alanine Transaminase Activity Assay
实验试剂 ALT Positive Control [ Detail : Lyophilised] ALT Substrate [ Detail : Lyophilised] Pyruvate Standard (100 nmol/µl) ALT Assay Buffer ALT Enzyme Mix [ Detail : Lyophilised
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