外切-β-1，4-葡聚糖酶活性检测试剂盒，用于对样本中C1活

外切-β-1,4-葡聚糖酶（C1）活性检测试剂盒（微量法）

货号：KTB3019-C

产品分类：核心产品｜细胞分析｜细胞代谢检测试剂盒

产品概述

CheKine™ 外切-β-1,4-葡聚糖酶（C1）活性检测试剂盒（Micro 1,4-β-D-Glucan Cellobilhydrolase Activity Assay Kit）是一款基于3,5-二硝基水杨酸法的微量检测试剂盒，适用于多种生物样本中C1酶活性的定量分析。该试剂盒通过检测C1催化微晶纤维素降解产生的还原糖含量，实现对C1酶活性的精确测定。

检测原理

外切-β-1,4-葡聚糖酶（C1，EC 3.2.1.91）可催化纤维素链末端的非还原性β-1,4-糖苷键水解，释放出纤维二糖和葡萄糖。本试剂盒利用C1酶促反应生成的还原糖与3,5-二硝基水杨酸发生显色反应，生成棕红色化合物，在540 nm处具有特征吸收峰。在一定浓度范围内，540 nm处吸光值的变化速率与C1酶活性呈正相关。

主要特点与优势

• 经验证适用于多种样本类型，包括动物组织与真菌
• 操作流程优化，简便高效，适合高通量筛选
• 提供48T与96T两种规格，灵活匹配不同实验规模
• 组分稳定，重现性良好

试剂盒组分

• Extraction Buffer
• Reagent I
• Reagent II
• Standard

样本要求

推荐使用新鲜样本。如不立即检测，样本可于-80℃保存数周。解冻过程需严格控制温度与时间，室温解冻应在4小时内完成。

保存条件与运输

试剂盒需在4℃避光保存，有效期为6个月。运输过程采用蓝冰冰袋保障低温条件。

规格与价格

注意事项

实验前请确保所有试剂、标准品及设备处于适宜温度。本品仅用于科学研究，不适用于人类或临床诊断。使用者应确保应用符合相关法律法规及知识产权要求。

别名：外切-β-1,4-葡聚糖酶（C1）；Exo-β-1,4-glucanase；C1 Activity Assay Kit

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Exo-β-1,4-glucanase (C1) Activity Assay Kit (Micro Method)

Catalog #: KTB3019-C

Product Classification: Core Products｜Cell Analysis｜Cell Metabolism Assay Kits

Overview

CheKine™ Exo-β-1,4-glucanase (C1) Activity Assay Kit (Micro 1,4-β-D-Glucan Cellobiohydrolase Activity Assay Kit) is a micro-assay kit based on the 3,5-dinitrosalicylic acid (DNS) method, designed for the quantitative analysis of C1 enzyme activity in various biological samples. This kit accurately measures C1 enzyme activity by detecting the amount of reducing sugars generated from the enzymatic degradation of microcrystalline cellulose catalyzed by C1.

Assay Principle

Exo-β-1,4-glucanase (C1, EC 3.2.1.91) catalyzes the hydrolysis of the non-reducing terminal β-1,4-glycosidic bonds in cellulose chains, releasing cellobiose and glucose. This kit utilizes a colorimetric reaction where the reducing sugars produced by the C1 enzymatic reaction react with 3,5-dinitrosalicylic acid to form a brown-red colored compound, which exhibits a characteristic absorption peak at 540 nm. Within a certain concentration range, the rate of change in absorbance at 540 nm is directly proportional to C1 enzyme activity.

Key Features & Advantages

• Validated for use with various sample types, including animal tissues and fungi
• Optimized, simple, and efficient procedure, suitable for high-throughput screening
• Available in 48T and 96T sizes to flexibly accommodate different experimental scales
• Stable components with excellent reproducibility

Kit Components

• Extraction Buffer
• Reagent I
• Reagent II
• Standard

Sample Requirements

Fresh samples are recommended. If not assayed immediately, samples can be stored at -80°C for several weeks. The thawing process must be carefully controlled regarding temperature and time; thawing at room temperature should be completed within 4 hours.

Storage Conditions & Shipping

The kit must be stored protected from light at 4°C and is stable for 6 months. Shipping is performed using blue ice packs to ensure maintenance of低温 conditions.

Specifications & Pricing

Precautions

Before the experiment, ensure all reagents, standards, and equipment are at appropriate temperatures. This product is intended for research use only and is not suitable for human or clinical diagnostic use. Users should ensure their application complies with relevant laws, regulations, and intellectual property requirements.

Also known as: Exo-β-1,4-glucanase (C1); Exo-β-1,4-glucanase; C1 Activity Assay Kit

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult with ACOBIO Company.