磷酸丙糖异构酶试剂盒，用于样本中TPI活性检测，微量法，TP

CheKine™ 磷酸丙糖异构酶（TPI）活性检测试剂盒（微量法）

货号：KTB1128-S

产品概述

CheKine™ Micro Triose-Phosphate Isomerase (TPI) Activity Assay Kit是一款用于精确检测多种生物样本（尤其是植物组织）中磷酸丙糖异构酶（TPI）活性的核心工具。该试剂盒基于比色法原理，通过监测340 nm处NADH的生成速率，灵敏反映TPI酶催化磷酸二羟丙`酮与3-磷酸甘油醛之间转化的活性，适用于细胞代谢研究及相关领域。

背景信息

磷酸丙糖异构酶（TPI）是植物叶绿体中Calvin循环的关键酶，参与光合作用过程，催化磷酸二羟丙`酮与3-磷酸甘油醛的相互转化。其活性直接影响碳代谢流与蔗糖合成效率。本试剂盒提供了一种稳定、可靠的检测方法，帮助研究人员深入探索植物生理及细胞能量代谢机制。

试剂盒组分

• Extraction Buffer I
• Extraction Buffer Ⅱ
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ

特点与优势

• 经验证可适用于多种样本类型
• 操作流程简便，检测过程高效
• 专为微量检测优化，节省样本与试剂

保存与运输

保存条件：-20℃避光保存，有效期6个月。

运输条件：蓝冰冰袋运输，确保试剂稳定性。

注意事项

• 建议使用新鲜样本。若需保存，请于-80℃条件下放置，避免反复冻融。
• 解冻样本时需控制温度与时间，室温解冻应在4小时内完成检测。
• 实验前请确保所有试剂及设备处于适宜温度，以保证检测结果的准确性。

规格与价格

警告：本产品仅供科研使用，不适用于人类或临床诊断。使用前请阅读并理解相关专利及许可限制，本公司不对因使用本产品导致的专利侵权或其他违规行为承担责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Micro Triose-Phosphate Isomerase (TPI) Activity Assay Kit

Catalog Number: KTB1128-S

Product Overview

The CheKine™ Micro Triose-Phosphate Isomerase (TPI) Activity Assay Kit is designed for the accurate detection of triose-phosphate isomerase (TPI) activity in a variety of biological samples, particularly plant tissues. Based on a colorimetric method, this kit sensitively measures the rate of NADH generation at 340 nm to reflect TPI enzymatic activity in catalyzing the interconversion between dihydroxyacetone phosphate and glyceraldehyde-3-phosphate. It is suitable for research in cellular metabolism and related fields.

Background

Triose-phosphate isomerase (TPI) is a key enzyme in the Calvin cycle within plant chloroplasts, participating in photosynthesis by catalyzing the interconversion of dihydroxyacetone phosphate and glyceraldehyde-3-phosphate. Its activity directly influences carbon metabolic flux and sucrose synthesis efficiency. This kit provides a stable and reliable assay method to support in-depth investigation into plant physiology and cellular energy metabolism mechanisms.

Kit Components

• Extraction Buffer I
• Extraction Buffer II
• Reagent I
• Reagent II
• Reagent III
• Reagent IV

Features & Advantages

• Validated for use with multiple sample types
• Simple procedure with high detection efficiency
• Optimized for micro-scale assays to conserve samples and reagents

Storage & Shipping

Storage Conditions: Store protected from light at -20°C; stable for 6 months.

Shipping Conditions: Shipped with blue ice packs to ensure reagent stability.

Precautions

• Fresh samples are recommended. If storage is necessary, keep samples at -80°C and avoid repeated freeze-thaw cycles.
• Thaw samples under controlled temperature and time; assays should be completed within 4 hours if thawed at room temperature.
• Ensure all reagents and equipment are at appropriate temperatures prior to the assay to guarantee accurate results.

Specifications & Pricing

Warning: This product is for research use only. Not intended for human or diagnostic use. Please review relevant patents and licensing restrictions prior to use. The company is not liable for patent infringement or non-compliance resulting from the use of this product.

Note: The above information is subject to change due to product improvements and updates. For detailed information, please contact Yakeyin Company.