过氧化氢酶活性检测试剂盒，用于对样本中CAT活性检测，CAT

CheKine™ Pro 过氧化氢酶（CAT）活性检测试剂盒（荧光法）

货号：KTB9040-C

产品分类：核心产品 - 细胞分析

产品概述

CheKine™ Pro Catalase (CAT) Fluorometric Activity Assay Kit是一款高特异性、高灵敏度的荧光法检测试剂盒，用于定量检测多种生物样本（包括动物/植物组织、细胞、血清（浆）及其他液体样本）中的过氧化氢酶（CAT）活性。该试剂盒采用经过优化的荧光探针法，可在535 nm激发波长和587 nm发射波长下精确测量未分解的过氧化氢含量，进而可靠反映CAT酶活性水平。

检测原理

在检测体系中，CAT酶分解H₂O₂产生水和氧气，未被分解的H₂O₂在酶和特异性荧光物质作用下发生反应，其所产生的荧光信号强度与H₂O₂浓度成正比，通过测定荧光强度即可推算出CAT的活性单位。

试剂盒组分

• Assay Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Standard

特点与优势

• 经验证适用于多种样本类型，具备良好的基质兼容性
• 操作流程简便，试剂预制程度高，有利于提高实验效率与一致性
• 采用荧光检测法，灵敏度较高，线性范围广

适用样本

动物/植物组织、细胞裂解液、血清（浆）及其他生物液体样本。

保存条件与运输

试剂盒需置于-20℃避光保存，有效期为6个月。运输全程采用冰袋（蓝冰）低温条件，确保试剂稳定性。

注意事项

• 推荐使用新鲜样本，若需保存，请于-80℃冻存，避免反复冻融。
• 样本解冻应在室温环境下快速完成，并于4小时内进行测定，以维持酶活性稳定。
• 实验前请确保所有试剂、设备及操作环境处于合适温度，以保证检测结果的准确性。

可选规格与价格

警告：本品仅供科研使用，不适用于人类或临床诊断。使用者应自行确认是否符合相关许可或专利规定，本公司不承担因产品使用而引起的专利侵权或其他违规行为责任。

别名/搜索关键词：CAT, 过氧化氢酶, Catalase, 过氧化氢酶活性分析, CheKine™ Pro CAT Assay Kit

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Pro Catalase (CAT) Activity Assay Kit (Fluorometric)

Catalog Number: KTB9040-C

Product Category: Core Products – Cell Analysis

Product Overview

The CheKine™ Pro Catalase (CAT) Fluorometric Activity Assay Kit is a highly specific and sensitive fluorometric assay kit designed for the quantitative detection of catalase (CAT) activity in various biological samples, including animal/plant tissues, cells, serum (plasma), and other liquid samples. Utilizing an optimized fluorescent probe method, this kit accurately measures the amount of undecomposed hydrogen peroxide at excitation and emission wavelengths of 535 nm and 587 nm, respectively, providing a reliable reflection of CAT enzyme activity levels.

Assay Principle

In the assay system, CAT enzyme decomposes H₂O₂ to produce water and oxygen. The undecomposed H₂O₂ reacts under the action of an enzyme and a specific fluorescent substance. The intensity of the resulting fluorescence signal is proportional to the concentration of H₂O₂. By measuring the fluorescence intensity, the activity units of CAT can be calculated.

Kit Components

• Assay Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Standard

Features & Advantages

• Validated for use with various sample types, offering excellent matrix compatibility
• Simplified procedure with highly pre-formulated reagents, enhancing experimental efficiency and consistency
• High sensitivity and broad linear range achieved through fluorometric detection

Applicable Samples

Animal/plant tissues, cell lysates, serum (plasma), and other biological liquid samples.

Storage Conditions & Shipping

The kit must be stored protected from light at -20°C and is stable for 6 months. It is shipped with ice packs (blue ice) under low-temperature conditions to ensure reagent stability.

Precautions

• Fresh samples are recommended. If storage is necessary, freeze at -80°C and avoid repeated freeze-thaw cycles.
• Thaw samples quickly at room temperature and perform the assay within 4 hours to maintain enzyme activity stability.
• Ensure all reagents, equipment, and the operating environment are at appropriate temperatures prior to the experiment to guarantee accurate results.

Available Specifications & Pricing

Warning: This product is for research use only and is not intended for human or clinical diagnostic use. Users are responsible for determining suitability regarding relevant licenses or patent regulations. The company assumes no liability for patent infringement or other violations arising from product use.

Aliases/Keywords: CAT, Catalase, Catalase Activity Assay, CheKine™ Pro CAT Assay Kit

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakonin Company.