D-乳酸脱氢酶活性检测试剂盒，用于对样本中D-LDH活性检测

D-乳酸脱氢酶（D-LDH）活性检测试剂盒

货号：KTB1111-C

产品类型：细胞代谢检测试剂盒

检测方法：比色法

产品概述

本试剂盒是一种用于定量检测样本中D-乳酸脱氢酶（D-LDH）活性的比色法检测工具。D-LDH是糖酵解代谢途径中的关键酶之一，广泛存在于动物组织、植物、微生物及培养细胞中，尤其在肾脏中具有较高活性水平。该试剂盒适用于多种样本类型的D-LDH活性分析，为细胞能量代谢研究提供可靠数据支撑。

检测原理

试剂盒通过酶促反应体系，将D-乳酸在D-LDH催化下转化为丙`酮酸，同时还原NAD+生成NADH。NADH在特定波长下具有光吸收特性，通过测定吸光度变化速率，可准确计算出D-LDH酶活性。

试剂盒组分

• Extraction Buffer
• Reagent Ⅰ
• Reagent Ⅱ
• Reagent III
• Reagent IV
• Reagent V
• Standard

特点与优势

• 特异性检测D-乳酸脱氢酶（D-LDH）活性
• 提供全面详实的样本制备与活性计算指南
• 适用于多种生物样本，操作流程清晰明确

注意事项

• 如不立即进行检测，样本可在-80℃条件下保存一个月
• 建议对样本进行多浓度稀释，以确保读数处于标准曲线线性范围内
• 推荐使用新鲜制备样本以确保实验结果的可靠性

保存与运输

保存条件：-20℃避光保存，有效期6个月。

运输条件：冰袋（蓝冰）低温运输。

规格与价格

警告：本产品仅限科研使用，不适用于人类或临床诊断。使用者应负责确保应用方式不侵犯任何专利权或许可证规定，厂家不对专利侵权或违规行为承担责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

D-Lactate Dehydrogenase (D-LDH) Activity Assay Kit

Catalog Number:KTB1111-C

Product Type:Cell Metabolism Assay Kit

Assay Method:Colorimetric Method

Product Overview

This kit is a colorimetric assay tool for quantitatively detecting the activity of D-lactate dehydrogenase (D-LDH) in samples. D-LDH is a key enzyme in the glycolytic metabolic pathway, widely present in animal tissues, plants, microorganisms, and cultured cells, with particularly high activity levels in the kidneys. This kit is suitable for D-LDH activity analysis in various sample types, providing reliable data support for research on cellular energy metabolism.

Assay Principle

The kit utilizes an enzymatic reaction system where D-lactate is converted to pyruvate under the catalysis of D-LDH, simultaneously reducing NAD+ to NADH. NADH exhibits specific light absorption characteristics at a particular wavelength. By measuring the rate of change in absorbance, the enzymatic activity of D-LDH can be accurately calculated.

Kit Components

• Extraction Buffer
• Reagent Ⅰ
• Reagent Ⅱ
• Reagent III
• Reagent IV
• Reagent V
• Standard

Features & Advantages

• Specifically detects D-lactate dehydrogenase (D-LDH) activity
• Provides comprehensive and detailed guidelines for sample preparation and activity calculation
• Suitable for a variety of biological samples, with a clear and straightforward operation procedure

Precautions

• If not assayed immediately, samples can be stored at -80°C for up to one month
• It is recommended to perform multi-concentration dilutions of the sample to ensure readings fall within the linear range of the standard curve
• Freshly prepared samples are recommended to ensure the reliability of experimental results

Storage & Shipping

Storage Conditions:Store protected from light at -20°C; valid for 6 months.

Shipping Conditions:Shipped at low temperatures with ice packs (blue ice).

Specifications & Pricing

Warning:This product is for research use only and is not intended for human or clinical diagnostic use. The user is responsible for ensuring that the manner of application does not infringe any patent rights or license provisions. The manufacturer assumes no liability for patent infringement or violations.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yakeyin Company.