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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4℃,避光保存 6 个月
- 保质期:
4℃,避光保存 6 个月
- 英文名:
Micro Ploygalacturonase (PG) Activity Assay Kit
- 库存:
999
- 供应商:
亚科因(Abbkine)
- 规格:
48 T/48 S/96 T/96 S
| 规格: | 48 T/48 S | 产品价格: | ¥1389.0 |
|---|---|---|---|
| 规格: | 96 T/96 S | 产品价格: | ¥2306.0 |
多聚半乳糖醛酸酶(PG)活性检测试剂盒(微量法)
货号:KTB1333-C
产品分类:细胞分析核心产品
产品类型:细胞代谢检测试剂盒
样本适用类型:植物组织、细菌、真菌及液体样本
产品概述
CheKine™ 多聚半乳糖醛酸酶(PG)活性检测试剂盒(Micro Ploygalacturonase Activity Assay Kit)是一种基于比色法的检测工具,用于定量分析多种生物样本中多聚半乳糖醛酸酶的活性。该酶广泛分布于植物、真菌和细菌中,并在细胞壁降解、果实软化、病原侵染等代谢过程中扮演关键角色。
检测原理
在多聚半乳糖醛酸酶(PG)催化下,多聚半乳糖醛酸被水解生成具有还原性醛基的半乳糖醛酸。还原性产物与 DNS 试剂发生反应,生成红棕色化合物,在 540 nm 波长处具有特征吸收峰。通过测定该波长吸光值的变化,即可准确计算出 PG 酶活性。
试剂盒组分
- Extraction Buffer
- Reagent I
- Reagent Ⅱ
- Standard
特点与优势
本试剂盒经过多方验证,可适用于多种样本类型,操作流程简便,重复性良好,适合中等至高通量的实验室研究使用。
注意事项
- 推荐使用新鲜样本。如不立即实验,样本可在 -80℃ 保存数周;解冻过程需控制温度与时间,室温解冻应在4小时内完成。
- 实验前请确保所有试剂及设备处于合适温度,以保证检测结果的准确性。
保存与运输
保存条件:4℃ 避光保存,有效期6个月。
运输方式:冰袋运输(蓝冰)。
规格与价格
| 规格 | 价格(元) | 货期 |
|---|---|---|
| 48 T/48 S | 1389 | 现货(次日发货) |
| 96 T/96 S | 2306 | 现货(次日发货) |
警告:本产品仅供科研使用,不适用于人类或临床诊断。使用本产品应遵守相关法律法规,不得用于专利或许可证禁止的用途。对于因使用本产品而导致的专利侵权或其他违规行为,本公司不承担任何责任。
注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。

Polygalacturonase (PG) Activity Assay Kit (Micro Method)
Catalog Number: KTB1333-C
Product Category: Core Products for Cell Analysis
Product Type: Cell Metabolism Assay Kit
Sample Applicable Types: Plant tissues, bacteria, fungi, and liquid samples
Product Overview
The CheKine™ Polygalacturonase (PG) Activity Assay Kit (Micro Method) is a colorimetric-based detection tool for the quantitative analysis of polygalacturonase activity in various biological samples. This enzyme is widely distributed in plants, fungi, and bacteria, and plays a key role in metabolic processes such as cell wall degradation, fruit softening, and pathogen infection.
Assay Principle
Under the catalysis of polygalacturonase (PG), polygalacturonic acid is hydrolyzed to generate galacturonic acid with reducing aldehyde groups. The reducing products react with DNS reagent to form a reddish-brown compound, which exhibits a characteristic absorption peak at 540 nm. By measuring the change in absorbance at this wavelength, PG enzyme activity can be accurately calculated.
Kit Components
- Extraction Buffer
- Reagent I
- Reagent II
- Standard
Features and Advantages
This kit has been extensively validated for use with various sample types. It features a simple operational procedure, good reproducibility, and is suitable for medium- to high-throughput laboratory research.
Precautions
- Fresh samples are recommended. If not used immediately, samples can be stored at -80°C for several weeks; thawing should be controlled for temperature and time, with room temperature thawing completed within 4 hours.
- Before the experiment, ensure all reagents and equipment are at appropriate temperatures to guarantee the accuracy of the detection results.
Storage and Shipping
Storage Conditions: Store at 4°C protected from light; valid for 6 months.
Shipping Method: Shipped with ice packs (blue ice).
Specifications and Pricing
| Specification | Price (CNY) | Lead Time |
|---|---|---|
| 48 T/48 S | 1389 | In stock (ships next day) |
| 96 T/96 S | 2306 | In stock (ships next day) |
Warning: This product is for research use only and is not intended for human or clinical diagnostic use. Use of this product must comply with relevant laws and regulations and must not be used for purposes prohibited by patents or licenses. The company assumes no liability for patent infringement or other violations resulting from the use of this product.
Note: The above information may change due to factors such as product optimization and upgrades. For specific details, please consult YaKeyin Company.
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