黄嘌呤氧化酶活性分析试剂盒，用于对样本中XOD活性分析，XO

黄嘌呤氧化酶活性分析试剂盒

英文名称：Micro Xanthine Oxidase Activity Assay Kit

产品货号：KTB1070-C

产品分类：核心产品 | 细胞代谢检测

产品概述

本试剂盒是一种基于比色法原理、专为检测多种生物样本中黄嘌呤氧化酶（Xanthine Oxidase, XOD）活性设计的高灵敏度分析工具。适用于血清、血浆、动植物组织/细胞裂解液以及其他生物体液样本，可广泛用于细胞代谢研究与氧化应激相关机制的分析。

检测原理

黄嘌呤氧化酶（XOD）是嘌呤分解代谢中的关键酶，能够催化次黄嘌呤转化为黄嘌呤，并进一步氧化生成尿酸，同时在该过程中产生超氧化物（O₂⁻）和过氧化氢（H₂O₂）等活性氧（ROS）。本试剂盒通过WST-8在XOD作用下的还原反应，产生水溶性甲臜染料，在450 nm附近具有强烈吸收峰，从而通过吸光度变化定量酶活性水平。

试剂盒组分

• 实验缓冲液
• 样品稀释液
• WST-8
• 增强剂
• 黄嘌呤

特点与优势

• 可广泛用于血清、血浆、动植物组织与细胞裂解液等多种样本类型；
• 操作简便，无需复杂预处理，适合高通量筛选；
• 灵敏度高，重现性好，结果稳定可靠。

规格与定价

规格	价格（元）	货期
48 T/48 S	289	现货，次日发货
96 T/96 S	439	现货，次日发货

保存与运输条件

试剂盒需避光保存于-20℃。请收到产品后参照说明书确认各组件的具体存储要求。在建议保存条件下，自生产发货之日起有效期为6个月。

运输方式：蓝冰冰袋运输，确保试剂活性稳定。

使用注意事项

• 使用前请轻轻低速离心，防止液体溅出；
• 若不能立即检测，样本可置于-80℃条件下保存；
• 建议依据实验规模选择合适的规格，避免反复冻融。

应用背景

黄嘌呤氧化酶（XOD）作为嘌呤代谢途径的关键酶，不仅参与尿酸生成，还在缺血再灌注损伤、氧化应激反应和多种肝脏疾病中发挥重要作用。血清XOD活性水平被认为是急性肝损伤（如黄疸）的敏感指标，在科研与体外研究中具有重要价值。

警告：本产品仅限用于科学研究，不适用于人类或临床诊断。使用者应负责确保应用方式不侵犯任何专利或许可协议。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Xanthine Oxidase Activity Assay Kit

English Name: Micro Xanthine Oxidase Activity Assay Kit

Catalog Number: KTB1070-C

Product Category: Core Products | Cellular Metabolism Assays

Product Overview

This kit is a highly sensitive colorimetric assay specifically designed to measure xanthine oxidase (XOD) activity in various biological samples. It is suitable for serum, plasma, animal/plant tissue or cell lysates, and other biological fluids, and is widely used in studies of cellular metabolism and oxidative stress-related mechanisms.

Assay Principle

Xanthine oxidase (XOD) is a key enzyme in purine catabolism, catalyzing the oxidation of hypoxanthine to xanthine and further to uric acid, while generating reactive oxygen species (ROS) such as superoxide anion (O₂⁻) and hydrogen peroxide (H₂O₂). This kit utilizes the reduction of WST-8 by XOD to produce a water-soluble formazan dye, which exhibits a strong absorption peak around 450 nm, enabling quantification of enzyme activity through absorbance changes.

Kit Components

• Assay Buffer
• Sample Dilution Buffer
• WST-8
• Enhancer
• Xanthine

Features & Advantages

• Broad applicability to various sample types including serum, plasma, animal/plant tissues, and cell lysates;
• Simple operation with no complex pretreatment, suitable for high-throughput screening;
• High sensitivity, excellent reproducibility, and reliable results.

Specifications & Pricing

Specification	Price (¥)	Delivery Time
48 T/48 S	289	In stock, shipped next day
96 T/96 S	439	In stock, shipped next day

Storage & Shipping Conditions

The kit should be stored protected from light at -20°C. Please refer to the instructions for specific storage requirements of each component upon receipt. Under recommended storage conditions, the kit is stable for 6 months from the date of shipment.

Shipping Method: Shipped with blue ice packs to ensure reagent stability.

Precautions

• Centrifuge gently at low speed before use to prevent splashing;
• If not assayed immediately, samples can be stored at -80°C;
• Select the appropriate kit size based on experimental scale to avoid repeated freeze-thaw cycles.

Background

Xanthine oxidase (XOD), a key enzyme in the purine metabolic pathway, is not only involved in uric acid production but also plays significant roles in ischemia-reperfusion injury, oxidative stress responses, and various liver diseases. Serum XOD activity is considered a sensitive indicator of acute liver injury (e.g., jaundice) and holds substantial value in scientific research and in vitro studies.

Warning: This product is for research use only. It is not intended for human use or clinical diagnosis. The user is responsible for ensuring that the application does not infringe any patents or licensing agreements.

Note: The above information is subject to change due to product optimization and updates. For detailed information, please contact Yakoyin Company.