Na⁺/K⁺-ATP试剂盒，用于样本中Na⁺/K⁺-ATP酶

Na⁺/K⁺-ATP酶活性检测试剂盒（比色法）

货号：KTB1800-E

本产品是一款基于比色法原理、用于精确检测多种生物样本中Na⁺/K⁺-ATP酶活性的核心试剂盒。Na⁺/K⁺-ATP酶是维持细胞膜离子梯度与电势平衡的关键酶，广泛参与细胞代谢过程，尤其在氧化磷酸化等能量代谢途径中发挥重要作用。

产品特点与优势

• 适用范围广泛：可测定血清、血浆、组织匀浆、细胞裂解液、细菌提取物、植物样本及其他生物体液中的Na⁺/K⁺-ATP酶活性。
• 操作流程清晰：提供标准化的样品制备流程与酶活性计算公式，便于用户快速开展实验并准确解读数据。
• 组分完整稳定：内含提取缓冲液（Extraction Buffer）、测定缓冲液（Assay Buffer）及多种高纯度试剂（Reagent I-VI），并提供标准品（Standard），确保检测可靠性。

检测原理

本试剂盒通过比色法检测Na⁺/K⁺-ATP酶催化ATP水解产生的无机磷，间接反映酶活性水平。该方法灵敏度高、操作简便，适用于多种样本类型的高通量筛选。

试剂盒组分

• Extraction Buffer
• Assay Buffer
• Reagent I
• Reagent II
• Reagent III
• Reagent IV
• Reagent V
• Reagent VI
• Standard

规格与定价

注意事项

• 需使用96孔板进行检测。
• 严禁混用不同批次或不同厂家的试剂组分。
• 样品处理须在冰上操作，并在当日完成测定，避免匀浆液反复冻融。
• 实验前请确保所有试剂及设备处于适宜温度。
• 严格使用无磷试管，避免磷污染对结果产生干扰。

保存与运输

产品需避光保存于-20℃。请参照说明书确认各组分具体存储条件。在推荐保存条件下，有效期为12个月（自发货日起计）。本品采用冰袋（蓝冰）运输。

警告

本产品仅限研究使用，不适用于人类或临床诊断。使用本品时请遵守相关法律法规与知识产权要求，本公司不对因使用本产品导致的专利侵权或其他违规行为承担责任。

如需了解更多技术细节或应用支持，请联系我们获取详细说明书与技术支持文档。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Na⁺/K⁺-ATPase Activity Assay Kit (Colorimetric Method)

Catalog Number: KTB1800-E

This product is a core kit based on the colorimetric principle for accurately detecting Na⁺/K⁺-ATPase activity in various biological samples. Na⁺/K⁺-ATPase is a key enzyme that maintains cellular membrane ion gradients and electrical potential balance, widely involved in cellular metabolic processes, especially playing a crucial role in energy metabolism pathways such as oxidative phosphorylation.

Features and Advantages

• Broad Applicability: Capable of measuring Na⁺/K⁺-ATPase activity in serum, plasma, tissue homogenates, cell lysates, bacterial extracts, plant samples, and other biological fluids.
• Clear Operational Procedure: Provides standardized sample preparation protocols and enzyme activity calculation formulas, facilitating rapid experimentation and accurate data interpretation.
• Complete and Stable Components: Includes Extraction Buffer, Assay Buffer, various high-purity reagents (Reagent I-VI), and a Standard, ensuring reliable detection.

Assay Principle

This kit employs a colorimetric method to detect inorganic phosphate produced by the hydrolysis of ATP catalyzed by Na⁺/K⁺-ATPase, indirectly reflecting enzyme activity levels. This method offers high sensitivity and simple operation, making it suitable for high-throughput screening of various sample types.

Kit Components

• Extraction Buffer
• Assay Buffer
• Reagent I
• Reagent II
• Reagent III
• Reagent IV
• Reagent V
• Reagent VI
• Standard

Specifications and Pricing

Precautions

• Detection must be performed using a 96-well plate.
• Do not mix reagents from different batches or manufacturers.
• Sample handling should be performed on ice, and assays should be completed on the same day to avoid repeated freeze-thaw cycles of homogenates.
• Ensure all reagents and equipment are at appropriate temperatures before starting the experiment.
• Strictly use phosphate-free tubes to avoid phosphorus contamination interfering with results.

Storage and Shipping

The product must be protected from light and stored at -20°C. Please refer to the manual for specific storage conditions of each component. The shelf life is 12 months (from the date of shipment) under recommended storage conditions. The product is shipped with ice packs (blue ice).

Warning

This product is for research use only and is not intended for human or clinical diagnostic use. When using this product, comply with relevant laws, regulations, and intellectual property requirements. The company is not liable for patent infringement or other violations resulting from the use of this product.

For more technical details or application support, please contact us for the detailed manual and technical support documents.

Note: The above information is subject to change due to product optimization and upgrades. For specific details, please consult Yakeyin Company.