细胞壁不溶性酸性转化酶活性检测试剂盒，用于对样本中B-AI活

细胞壁不溶性酸性转化酶(B-AI)活性检测试剂盒

货号：KTB2293-C

产品类型：细胞代谢检测试剂盒

检测方法：比色法

产品概述

本试剂盒是一款专为植物组织样本中细胞壁不溶性酸性转化酶(B-AI)活性检测而设计的高灵敏度工具。B-AI是植物蔗糖代谢中的关键酶，主要存在于细胞间隙并与细胞壁结合，在蔗糖分解和韧皮部卸载过程中发挥重要作用。通过特异性催化蔗糖生成还原糖，并与3,5-二硝基水杨酸反应形成在510 nm处具有特征吸收的棕红色化合物，实现对B-AI活性的准确、稳定定量。

应用领域

• 植物生理与代谢研究
• 蔗糖代谢途径分析
• 细胞壁相关酶功能研究
• 库源关系调控机制探讨

试剂盒组分

• Extraction Buffer I
• Extraction Buffer Ⅱ
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Standard

规格与定价

保存与运输

保存条件：4℃避光保存，有效期6个月。

运输条件：蓝冰冰袋运输，保障试剂稳定性。

使用注意事项

• 建议使用新鲜样本，若需保存，请于-80℃存放，避免反复冻融。
• 解冻过程应在室温下快速完成（4小时内），以保证酶活性不受影响。
• 实验前请确保所有试剂及设备处于适宜温度，以保证反应稳定性。

警告

本产品仅限科研使用，不适用于人类或临床诊断。使用者应自行负责确保应用行为不侵犯任何专利或许可协议，厂家不对专利侵权或其他违规行为承担责任。

更多技术细节及协议优化建议，请联系技术支持。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Cell Wall-Bound Acid Invertase (B-AI) Activity Assay Kit

Catalog Number: KTB2293-C

Product Type: Cellular Metabolism Assay Kit

Assay Method: Colorimetric Method

Product Overview

This kit is a highly sensitive tool specifically designed for the detection of cell wall-bound acid invertase (B-AI) activity in plant tissue samples. B-AI is a key enzyme in plant sucrose metabolism, primarily located in the intercellular space and bound to the cell wall, playing a crucial role in sucrose cleavage and phloem unloading. By specifically catalyzing the conversion of sucrose to reducing sugars, which then react with 3,5-dinitrosalicylic acid to form a brownish-red compound with characteristic absorption at 510 nm, it enables accurate and stable quantification of B-AI activity.

Applications

• Plant Physiology and Metabolism Research
• Sucrose Metabolic Pathway Analysis
• Functional Studies of Cell Wall-Associated Enzymes
• Investigation of Source-Sink Relationship Regulation Mechanisms

Kit Components

• Extraction Buffer I
• Extraction Buffer Ⅱ
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Standard

Specifications and Pricing

Storage and Shipping

Storage Conditions: Store protected from light at 4°C; valid for 6 months.

Shipping Conditions: Shipped with blue ice packs to ensure reagent stability.

Precautions for Use

• Fresh samples are recommended. If storage is necessary, store at -80°C and avoid repeated freeze-thaw cycles.
• The thawing process should be completed rapidly at room temperature (within 4 hours) to ensure enzyme activity is not compromised.
• Before the experiment, ensure all reagents and equipment are at appropriate temperatures to guarantee reaction stability.

Warning

This product is for research use only and is not intended for human or clinical diagnostic use. The user is solely responsible for ensuring that application activities do not infringe upon any patents or licensing agreements. The manufacturer assumes no liability for patent infringement or other violations.

For more technical details and protocol optimization suggestions, please contact technical support.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Yacoin Biotechnology.