SP试剂盒，用于样本中蔗糖磷酸化酶活性检测，SP Activ

CheKine™ 蔗糖磷酸化酶（SP）活性检测试剂盒（微量法）

货号：KTB3120-E

CheKine™ Micro Sucrose Phosphorylase (SP) Activity Assay Kit是一款高效、可靠的酶活性检测工具，专为植物组织和真菌样本中蔗糖磷酸化酶（SP）活性的精准定量而设计。本试剂盒采用比色法原理，通过监测340 nm处NADPH吸光度的变化，实现对SP酶活性的高灵敏度分析。

产品特点

• 广泛的样本兼容性：经验证适用于多种植物组织及真菌样本。
• 操作流程简便：优化的反应体系，减少操作步骤，提升实验效率。
• 高重复性：精心配比的试剂组分，确保实验结果稳定可靠。

试剂盒组分

• Extraction Buffer
• Assay Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ
• Reagent Ⅴ
• Reagent VI
• Reagent VII

技术背景

蔗糖磷酸化酶（Sucrose Phosphorylase, SP, EC2.4.1.7）属于糖基水解酶13家族，广泛存在于微生物和植物中。它能够催化蔗糖与无机磷酸盐反应生成1-磷酸葡萄糖，进而参与多种糖苷化合物的生物合成。本试剂盒通过多酶偶联反应，将NADP+还原为NADPH，并通过其于340 nm处的吸光度增长速率精确反映SP活性。

注意事项

• 推荐使用新鲜样本。若需保存，请于-80℃冷冻，避免反复冻融。
• 解冻样本时建议在低温环境下进行，室温解冻需在4小时内完成实验。
• 实验前请确保所有试剂及设备处于适宜温度，以保证反应效率及数据准确性。

保存与运输

• 保存条件：-20℃避光保存，有效期6个月。
• 运输方式：冰袋（蓝冰）运输，确保试剂稳定性。

订购信息

规格	价格（元）	货期
48 T/24 S	658	现货（次日发货）
96 T/48 S	1098	现货（次日发货）

警告：本产品仅用于科学研究，不适用于人类或临床诊断。使用者应确保应用方式不侵犯任何专利或许可协议，本公司不对因使用本产品导致的专利侵权或其他违规行为承担责任。

别名：蔗糖磷酸化酶（SP）；SP Activity Assay Kit

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Micro Sucrose Phosphorylase (SP) Activity Assay Kit

Cat. No.: KTB3120-E

The CheKine™ Micro Sucrose Phosphorylase (SP) Activity Assay Kit is an efficient and reliable tool designed for the accurate quantification of sucrose phosphorylase (SP) activity in plant tissues and fungal samples. This kit utilizes a colorimetric method, monitoring the change in absorbance of NADPH at 340 nm to achieve high-sensitivity analysis of SP enzyme activity.

Features

• Broad Sample Compatibility: Validated for use with various plant tissues and fungal samples.
• Simplified Procedure: Optimized reaction system reduces operational steps and improves experimental efficiency.
• High Reproducibility: Precisely formulated reagent components ensure stable and reliable results.

Kit Components

• Extraction Buffer
• Assay Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Reagent Ⅳ
• Reagent Ⅴ
• Reagent VI
• Reagent VII

Technical Background

Sucrose Phosphorylase (SP, EC2.4.1.7) belongs to the glycoside hydrolase family 13 and is widely present in microorganisms and plants. It catalyzes the reaction of sucrose with inorganic phosphate to produce glucose-1-phosphate, thereby participating in the biosynthesis of various glycoside compounds. This kit employs a multi-enzyme coupled reaction to reduce NADP+ to NADPH, with the rate of increase in absorbance at 340 nm accurately reflecting SP activity.

Precautions

• Fresh samples are recommended. If storage is necessary, freeze at -80°C and avoid repeated freeze-thaw cycles.
• Thaw samples under low-temperature conditions; if thawed at room temperature, complete the experiment within 4 hours.
• Ensure all reagents and equipment are at appropriate temperatures before the experiment to guarantee reaction efficiency and data accuracy.

Storage and Shipping

• Storage Conditions: Store at -20°C protected from light; valid for 6 months.
• Shipping Method: Shipped with ice packs (blue ice) to ensure reagent stability.

Ordering Information

Specification	Price (¥)	Lead Time
48 T/24 S	658	In Stock (Ships Next Day)
96 T/48 S	1098	In Stock (Ships Next Day)

Warning: This product is intended for research use only and is not suitable for human or clinical diagnostics. Users should ensure that the application does not infringe any patents or licensing agreements. The company is not liable for any patent infringements or other violations resulting from the use of this product.

Aliases: Sucrose Phosphorylase (SP); SP Activity Assay Kit

Note: The above information is subject to change due to product optimization and upgrades. For specific details, please contact Abbkine.