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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4℃,避光保存 6 个月
- 保质期:
4℃,避光保存 6 个月
- 英文名:
Micro Plant Root Vitality (TTC-Method) Assay Kit
- 库存:
999
- 供应商:
亚科因(Abbkine)
- 规格:
48 T/24 S/96 T/48 S
| 规格: | 48 T/24 S | 产品价格: | ¥278.0 |
|---|---|---|---|
| 规格: | 96 T/48 S | 产品价格: | ¥458.0 |
植物根系活力检测试剂盒 (TTC法,微量法)
货号:KTB3025-C
英文名称:Micro Plant Root Vitality (TTC-Method) Assay Kit
产品概述
CheKine™ 植物根系活力检测试剂盒(TTC法,微量法)是一款基于比色法原理设计的高品质检测工具,专为植物组织样本中根系活力的精准、快速检测而开发。该试剂盒通过测定植物脱氢酶(Plant dehydrogenase, PDHA)活性,有效反映植物根系细胞的代谢状态与生理活性,为植物生理学研究、农业科研及环境监测提供关键数据支持。
检测原理
植物根系细胞中的脱氢酶在呼吸过程中可将受氢体2,3,5-氯化三苯基四氮唑(TTC)还原为红色产物三苯基甲替(TFF),该物质在485 nm波长处具有最大吸收峰。通过分光光度法测定其吸光度值,即可准确计算植物根系脱氢酶活性,进而评估根系活力水平。
试剂盒组分
- Reagent ⅠA
- Reagent ⅠB
- Reagent Ⅱ
- Reagent Ⅲ
- Standard
产品特点与优势
- 采用微量法设计,节省样本和试剂用量,适合高通量筛选
- 操作流程优化,反应稳定,重复性好
- 提供标准品,便于定量分析与结果校准
适用样本类型
适用于多种植物组织样本,建议使用新鲜样本进行检测。若需保存,样本可在-80℃条件下贮存数周;解冻后应在4小时内完成实验,以保证检测结果的准确性。
保存与运输条件
保存条件:4℃避光保存,有效期6个月
运输条件:冰袋运输(蓝冰)
规格与价格
| 规格 | 价格(元) | 货期 |
|---|---|---|
| 48 T/24 S | 278 | 现货(次日发货) |
| 96 T/48 S | 458 | 现货(次日发货) |
注意事项
- 实验前请确保所有试剂及设备处于适宜温度
- 解冻样本时应严格控制温度与时间,避免反复冻融
- 本品仅供科研使用,不适用于人类或临床诊断
警告:本产品仅用于研究目的,不建议用于人类或临床诊断。使用本产品应遵守相关法律法规,避免侵犯专利权或其他许可协议。对于因使用本产品可能导致的专利侵权或其他违规行为,本公司不承担任何责任。
相关搜索关键词
植物根系活力、TTC法、脱氢酶活性检测、植物代谢检测、根系生理研究、TPI检测
注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。

Plant Root Vitality Assay Kit (TTC Method, Microscale)
Cat. No.:KTB3025-C
English Name:Micro Plant Root Vitality (TTC-Method) Assay Kit
Product Overview
CheKine™ Plant Root Vitality Assay Kit (TTC Method, Microscale) is a high-quality colorimetric assay designed for accurate and rapid detection of root vitality in plant tissue samples. By measuring plant dehydrogenase (PDHA) activity, this kit effectively reflects the metabolic state and physiological activity of plant root cells, providing critical data support for plant physiology research, agricultural science, and environmental monitoring.
Assay Principle
Dehydrogenases in plant root cells reduce the hydrogen acceptor 2,3,5-triphenyltetrazolium chloride (TTC) to the red product triphenylformazan (TFF) during respiration. This product has a maximum absorption peak at 485 nm. By measuring the absorbance spectrophotometrically, the activity of plant root dehydrogenases can be accurately calculated, thereby assessing the level of root vitality.
Kit Components
- Reagent ⅠA
- Reagent ⅠB
- Reagent Ⅱ
- Reagent Ⅲ
- Standard
Features and Advantages
- Microscale design reduces sample and reagent consumption, suitable for high-throughput screening
- Optimized protocol with stable reaction and high reproducibility
- Includes standard for quantitative analysis and result calibration
Applicable Sample Types
Suitable for various plant tissue samples. Fresh samples are recommended for detection. If storage is necessary, samples can be stored at -80°C for several weeks; experiments should be completed within 4 hours after thawing to ensure accurate results.
Storage and Shipping Conditions
Storage: Store at 4°C protected from light; stable for 6 months
Shipping: Shipped with ice packs (blue ice)
Specifications and Pricing
| Specification | Price (¥) | Lead Time |
|---|---|---|
| 48 T/24 S | 278 | In stock (ships next day) |
| 96 T/48 S | 458 | In stock (ships next day) |
Precautions
- Ensure all reagents and equipment are at appropriate temperatures before starting the experiment
- Strictly control temperature and time during sample thawing; avoid repeated freeze-thaw cycles
- For research use only. Not intended for human or clinical diagnostic use
Warning: This product is for research purposes only and is not recommended for human or clinical diagnosis. Use of this product should comply with relevant laws and regulations and avoid infringement of patents or other licensing agreements. The company assumes no liability for any patent infringement or other violations that may result from the use of this product.
Related Search Keywords
Plant root vitality, TTC method, Dehydrogenase activity assay, Plant metabolism detection, Root physiology research, TPF detection
Note: The above information is subject to change due to product optimization and upgrades. For specific details, please consult AbcamBio.
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文献和实验1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
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