Glucoamylase试剂盒，用于样本中糖化酶活性检测，G

CheKine™ 糖化酶（Glucoamylase）活性检测试剂盒（微量法）

货号：KTB1570-E

产品分类：核心产品 · 细胞代谢检测

样本类型：动植物组织、细菌和细胞、血清（浆）及其他液体样本

产品概述

本试剂盒是一种基于比色法原理开发的糖化酶（Glucoamylase）活性检测工具，适用于多种生物样本中糖化酶活性的准确测定。糖化酶（EC 3.2.1.3），又称γ-淀粉酶，是一种能够从淀粉及糖原非还原末端水解α-1,4和α-1,6糖苷键的关键酶，广泛应用于工业及生命科学研究领域。

检测原理

试剂盒通过酶促反应将可溶性淀粉转化为葡萄糖，葡萄糖在碱性条件下与3,5-二硝基水杨酸反应生成红棕色化合物，在540 nm波长处测定吸光度值。葡萄糖含量与颜色深浅成正比，从而精确计算出样本中糖化酶的活性。

主要特点与优势

• 经验证适用于多种样本类型，包括动植物组织、细菌、细胞及液体样本；
• 操作流程清晰简便，提供详尽的样品制备与结果计算指南；
• 试剂盒性能稳定，重现性良好。

试剂盒组分

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Standard

规格与定价

规格	价格（元）	货期
48 T/48 S	906	现货（次日发货）
96 T/96 S	1516	现货（次日发货）

保存与运输

保存条件：4℃避光保存，有效期6个月。

运输方式：冰袋（蓝冰）运输，确保试剂活性。

注意事项

• 推荐使用新鲜样本，如需保存请置于-80℃，避免反复冻融；
• 解冻后的样本应在4小时内完成测定；
• 实验前请确保所有试剂及设备处于合适温度，以保证反应准确性。

使用声明：本产品仅限科学研究使用，不可用于人类或临床诊断。使用者应自行确保应用方式不侵犯任何专利或许可权利。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Glucoamylase Activity Assay Kit (Micro Method)

Catalog Number: KTB1570-E

Product Classification: Core Products · Cell Metabolism Assays

Sample Types: Animal and plant tissues, bacteria and cells, serum (plasma), and other liquid samples

Product Overview

This kit is a colorimetric-based tool developed for the accurate determination of glucoamylase activity in various biological samples. Glucoamylase (EC 3.2.1.3), also known as γ-amylase, is a key enzyme capable of hydrolyzing α-1,4 and α-1,6 glycosidic bonds from the non-reducing ends of starch and glycogen, widely used in industrial and life science research.

Assay Principle

The kit converts soluble starch into glucose via enzymatic reaction. Under alkaline conditions, glucose reacts with 3,5-dinitrosalicylic acid to form a reddish-brown compound, and the absorbance is measured at 540 nm wavelength. The glucose content is proportional to the color intensity, allowing precise calculation of glucoamylase activity in the sample.

Key Features and Advantages

• Validated for various sample types, including animal and plant tissues, bacteria, cells, and liquid samples;
• Clear and straightforward operating procedure with detailed guidelines for sample preparation and result calculation;
• Stable performance and excellent reproducibility.

Kit Components

• Extraction Buffer
• Reagent I
• Reagent II
• Standard

Specifications and Pricing

Specification	Price (CNY)	Lead Time
48 T/48 S	906	In stock (shipped next day)
96 T/96 S	1516	In stock (shipped next day)

Storage and Shipping

Storage Conditions: Store at 4°C protected from light; stable for 6 months.

Shipping Method: Shipped with ice packs (blue ice) to ensure reagent activity.

Precautions

• Fresh samples are recommended; if storage is necessary, keep at -80°C and avoid repeated freeze-thaw cycles;
• Thawed samples should be assayed within 4 hours;
• Ensure all reagents and equipment are at appropriate temperatures before the experiment to guarantee reaction accuracy.

Usage Statement: This product is intended for research use only and not for human or clinical diagnosis. Users are responsible for ensuring that their application does not infringe any patents or licensing rights.

Note: The above information is subject to change due to product optimization and upgrades. For specific details, please consult AcroBio Company.