Chitinase试剂盒，用于样本中几丁质酶活性检测，Chi

几丁质酶（EC 3.2.1.14）在甲壳类动物外壳、真菌细胞壁等几丁质丰富组织中广泛存在。其活性与抗真菌机制密切相关，是细胞代谢与真菌病害防控领域的重要研究对象。

别名/搜索关键词：几丁质酶，Chitinase Activity Assay Kit

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Micro Chitinase Activity Assay Kit (KTB3012-E)

This kit is a highly sensitive microplate-based assay tool designed for detecting chitinase activity in animal tissues, fungal samples, and liquid specimens. It is suitable for research on cell metabolism and related biological processes in both scientific and industrial settings.

Product Description

CheKine™ Micro Chitinase Activity Assay Kit employs the principle of enzymatic hydrolysis. Chitinase catalyzes the hydrolysis of chitin to generate N-acetyl-D-glucosamine, which subsequently reacts with DNS reagent to produce a brown-red compound with a characteristic absorption peak at 540 nm. The rate of absorbance change accurately reflects chitinase activity levels.

Key Features and Advantages

• Validated for various sample types: animal tissues, fungal samples, and liquid specimens;
• Simple procedure with high reaction stability and reproducibility;
• Complete set of components provided, including Extraction Buffer, various Reagents, and Standard, ready to use upon opening;
• Detection sensitivity meets the needs of most common experimental requirements.

Kit Components

• Extraction Buffer
• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Standard

Sample Handling Recommendations

Fresh samples are recommended for detection. If storage is necessary, samples should be kept at -80°C and can be stored for several weeks. Thawing conditions must be controlled before assay; if thawed at room temperature, complete the procedure within 4 hours to avoid loss of enzyme activity.

Storage and Shipping

This product must be stored protected from light at 4°C and is stable for 6 months. It is shipped with blue ice packs throughout transit to ensure reagent stability.

Specifications and Pricing

Precautions

Ensure all reagents and equipment are at appropriate temperatures before starting the experiment. This product is for research use only, not for human or clinical diagnostic use. Please review relevant patents and licensing information prior to use. The manufacturer is not liable for patent infringement or regulatory violations resulting from product usage.

Background Information

Chitinase (EC 3.2.1.14) is widely present in chitin-rich tissues such as crustacean shells and fungal cell walls. Its activity is closely related to antifungal mechanisms, making it an important subject of study in cell metabolism and fungal disease control.

Alias/Search Keywords: Chitinase, Chitinase Activity Assay Kit

Note: The above information is subject to change due to product optimization and upgrades. For detailed information, please consult Yakonin Company.