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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃,避光保存 6 个月。
- 保质期:
-20℃,避光保存 6 个月。
- 英文名:
Micro D-Lactic acid(D-LA)Assay Kit
- 库存:
999
- 供应商:
亚科因(Abbkine)
- 规格:
96 T/96 S
D-乳酸含量检测试剂盒 (Micro D-Lactic acid (D-LA) Assay Kit)
货号:KTB1112-C
规格:96 T/96 S
价格:1248元
库存状态:现货,次日发货
产品概述
本试剂盒是一款基于比色法原理,专业用于精准检测血清(浆)、细胞、细菌或组织提取物中D-乳酸(D-LA)含量的核心产品。D-乳酸作为生物体代谢过程中的关键中间产物,其水平变化与糖酵解代谢状态、能量代谢及多种生理病理过程密切相关。本产品为细胞代谢研究,特别是糖酵解途径分析提供了可靠的检测工具。
检测原理与应用
试剂盒通过特定的酶促反应体系,将样本中的D-LA转化为可被比色测定的产物,通过测定吸光度值的变化,精确计算出D-LA的浓度。该方案适用于多种样本类型的基础研究。
试剂盒组分
- Extraction Buffer
- Reagent I
- Reagent II
- Reagent III
- Reagent IV
- Standard
产品特点与优势
- 特异性强:针对D-乳酸异构体进行检测,减少L-乳酸的干扰。
- 应用广泛:适用于血清、血浆、细胞裂解液、细菌培养物及组织匀浆等多种样本基质。
- 操作便捷:提供了从样品制备到结果计算的详尽实验方案与计算公式,简化操作流程。
- 结果稳定:优化的反应体系确保检测的重复性与准确性。
保存与运输
保存条件:-20℃避光保存,有效期6个月。
运输条件:采用冰袋(蓝冰)低温运输,以确保试剂稳定性。
注意事项
- 为获得最佳检测效果,推荐使用新鲜制备的样本。若需保存,样本可在-80℃环境下储存数周。
- 测定前若需解冻,请在室温环境下进行并确保在4小时内完成解冻,以避免反复冻融或长时间解冻对样品活性的影响。
- 本产品仅供科学研究使用,不适用于人类或临床诊断。
警告:本产品仅用于研究用途。使用本产品不应违反任何专利或许可证规定。对于因使用本产品可能导致的专利侵权或其他违规行为,我们不承担任何责任。
注意:以上信息可能随着产品优化升级等因素产生变动,具体信息请咨询亚科因公司。

D-Lactic Acid Assay Kit (Micro D-Lactic acid (D-LA) Assay Kit)
Catalog Number: KTB1112-C
Specification: 96 T/96 S
Price: 1248 CNY
Stock Status: In stock, ships next day
Product Overview
This kit employs a colorimetric method for the accurate quantification of D-lactic acid (D-LA) levels in serum/plasma, cells, bacteria, or tissue extracts. As a key intermediate in metabolic pathways, D-LA levels are closely associated with glycolytic flux, energy metabolism, and various physiological and pathological processes. This product provides a reliable tool for cellular metabolism research, particularly in the analysis of glycolytic pathways.
Assay Principle and Application
The kit utilizes a specific enzymatic reaction system to convert D-LA in the sample into a colorimetrically measurable product. The concentration of D-LA is precisely calculated by measuring the change in absorbance. This protocol is suitable for fundamental research using various sample types.
Kit Components
- Extraction Buffer
- Reagent I
- Reagent II
- Reagent III
- Reagent IV
- Standard
Features and Advantages
- High Specificity: Targets the D-lactic acid isomer, minimizing interference from L-lactic acid.
- Broad Applicability: Suitable for various sample matrices including serum, plasma, cell lysates, bacterial cultures, and tissue homogenates.
- User-Friendly: Provides a detailed protocol from sample preparation to result calculation, streamlining the experimental procedure.
- Reliable Results: An optimized reaction system ensures repeatable and accurate detection.
Storage and Shipping
Storage Conditions: Store protected from light at -20°C; stable for 6 months.
Shipping Conditions: Shipped with ice packs (blue ice) to maintain reagent stability.
Precautions
- For optimal results, it is recommended to use freshly prepared samples. If storage is necessary, samples can be kept at -80°C for several weeks.
- If thawing is required before assay, perform it at room temperature and ensure completion within 4 hours to avoid the impact of repeated freeze-thaw cycles or prolonged thawing on sample integrity.
- This product is for research use only. Not intended for human or clinical diagnostic use.
Warning: This product is for research purposes only. Use of this product should not infringe any patents or licensing agreements. We assume no liability for patent infringement or other violations that may result from the use of this product.
Note: The above information is subject to change due to product optimization and updates. For specific details, please consult YaKeyin Company.
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文献和实验1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
相关专题 在蛋白质定量试验中,最常用的方法是BCA法蛋白定量 。那么为什么我们要选择用BCA法?而且常常使用到BCA蛋白定量试剂盒(BCA Protein Assay Kit)。因为BCA法蛋白定量具有很多优点,如1.实验操作十分简单方便;2.实验耗时短,快速;3.试剂灵敏度高,实验结果准确;4.所用的试剂稳定性好,而且经济实用,抗干扰能力强等。 实验原理: BCA(bicinchoninic acid)法蛋白浓度定量
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