HK试剂盒，用于样本中己糖激酶活性检测，HK Activit

己糖激酶(HK)活性检测试剂盒 | Micro Hexokinase (HK) Activity Assay Kit

货号：KTB1123-E
产品分类：核心产品 – 细胞代谢分析
检测类型：糖酵解代谢关键酶活性分析

产品概述

本试剂盒提供一种高灵敏度的比色法分析方案，用于定量检测多种生物样本（包括血清、血浆、动植物组织、细胞上清、细菌及培养细胞）中的己糖激酶（HK）活性。HK是糖酵解途径的起始关键酶，催化葡萄糖磷酸化生成6-磷酸葡萄糖，该产物是糖酵解与磷酸戊糖途径的重要枢纽节点。

检测原理

基于WST-8还原显色反应，通过偶联NADH生成速率，间接反映HK酶活性水平。该体系稳定性高，线性关系良好，适用于精确的酶动力学研究。

试剂盒组分

• Extraction Buffer
• Assay Buffer
• Substrate Mix
• Enzyme Mix
• WST-8
• Enhancer
• NADH Standard

特点与优势

• 广谱适用性：适用于多种生物样本类型；
• 操作便捷：提供详尽的样本制备指南与活性计算公式；
• 线性范围宽：检测范围为31.25–2000 μM，满足大多数实验需求；
• 高重现性：优化反应体系，结果稳定可靠。

技术参数

规格	检测数量	价格（元）	货期
规格1	48 T/24 S	348	现货（当天发货）
规格2	96 T/48 S	406	现货（次日发货）

保存与运输

保存条件：-20℃避光保存，各组分具体存储条件请参见说明书。有效期自发货日起12个月。
运输条件：蓝冰冰袋运输，保障试剂稳定性。

注意事项

• 请勿混用不同批次或不同厂家的试剂组分；
• 部分组分具挥发性，操作时需佩戴口罩与手套，使用后及时盖紧瓶盖；
• 实验前请确保所有试剂及设备均达到适宜温度。

警告：本产品仅用于科学研究用途，不适用于人类或临床诊断。使用者应自行确保应用方式不违反相关专利或许可规定，本公司不对因使用本产品导致的专利侵权或其他违规行为承担责任。

别名/关键词：己糖激酶, HK, 己糖激酶活性, HK活性, 己糖激酶活性检测试剂盒, HK活性试剂盒, hexokinase, HK检测

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Hexokinase (HK) Activity Assay Kit | Micro Hexokinase (HK) Activity Assay Kit

Catalog Number: KTB1123-E
Product Category: Core Products – Cell Metabolism Analysis
Assay Type: Key Enzyme Activity Analysis in Glycolytic Metabolism

Product Overview

This kit provides a highly sensitive colorimetric assay for the quantitative detection of hexokinase (HK) activity in various biological samples, including serum, plasma, animal and plant tissues, cell supernatants, bacteria, and cultured cells. HK is the key initiating enzyme of the glycolytic pathway, catalyzing the phosphorylation of glucose to glucose-6-phosphate, a critical hub connecting glycolysis and the pentose phosphate pathway.

Assay Principle

The assay is based on the WST-8 reduction colorimetric reaction, which indirectly reflects HK enzyme activity levels by coupling with the rate of NADH generation. This system exhibits high stability and excellent linearity, making it suitable for precise enzyme kinetic studies.

Kit Components

• Extraction Buffer
• Assay Buffer
• Substrate Mix
• Enzyme Mix
• WST-8
• Enhancer
• NADH Standard

Features & Advantages

• Broad Applicability: Suitable for various biological sample types;
• User-Friendly Operation: Provides detailed sample preparation guidelines and activity calculation formulas;
• Wide Linear Range: Detection range of 31.25–2000 μM, meeting the needs of most experiments;
• High Reproducibility: Optimized reaction system ensures stable and reliable results.

Technical Parameters

Specification	Assay Number	Price (¥)	Lead Time
Specification 1	48 T/24 S	348	In Stock (Ships Same Day)
Specification 2	96 T/48 S	406	In Stock (Ships Next Day)

Storage & Shipping

Storage Conditions: Store protected from light at -20°C. Refer to the manual for specific storage conditions for each component. The shelf life is 12 months from the date of shipment.
Shipping Conditions: Shipped with blue ice packs to ensure reagent stability.

Precautions

• Do not mix reagents from different batches or manufacturers;
• Some components are volatile; wear a mask and gloves during operation, and tightly close bottle caps after use;
• Ensure all reagents and equipment reach the appropriate temperature before starting the experiment.

Warning: This product is for research use only and is not intended for human or clinical diagnostic use. The user is responsible for ensuring that the application does not infringe relevant patents or licensing regulations. The company is not liable for patent infringement or other violations resulting from the use of this product.

Aliases/Keywords: Hexokinase, HK, Hexokinase Activity, HK Activity, Hexokinase Activity Assay Kit, HK Activity Kit, hexokinase, HK Assay

Note: The above information is subject to change due to product optimization and upgrades. Please contact Yakeyin Company for specific details.