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- bs-19362R-PerCP-Cy5.5
- 2025年12月01日
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100ul
| 产品编号 | bs-19362R-PerCP-Cy5.5 |
| 英文名称 | SACM1L Rabbit pAb, PerCP-Cy5.5 conjugated |
| 中文名称 | PerCP-Cy5.5标记的肌动蛋白抑制因子1抗体 |
| 英文别名 | Phosphatidylinositide phosphatase SAC1; SAC1; SAC1 suppressor of actin mutations 1-like(yeast); SAC1_HUMAN; SACM1L; suppressor of actin 1; Suppressor of actin mutations 1-like protein. |
| 产品应用 | ICC/IF=1:50-200, IF=1:100-500 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SACM1L |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Signaling Pathway > Lipid Signaling > Lipid Phosphatases |
| 亚细胞定位 | Endoplasmic reticulum membrane. |
| 组织特异性 | Detected in heart, brain, lung, liver, kidney, pancreas and testis. |
| 相似性 | Contains 1 SAC domain. |
| 功能 | Phosphoinositide phosphatase that hydrolyzes PtdIns(3)P and PtdIns(4)P. Has low activity towards PtdIns(3,5)P2. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 背景资料 | The Saccharomyces cerevisiae SAC1 gene modulates yeast actin function and alleviates the essential requirement for phosphatidylinositol transfer protein (sec14p) activity in Golgi secretory function. The SAC1 gene product (Sac1p) is an integral membrane lipid phosphatase of the endoplasmic reticulum (ER) and the Golgi complex and contains a Sac phosphatase domain (1-2). Sac1p functions in a wide range of cellular processes including inositol metabolism, actin cytoskeletal organization, endoplasmic reticulum ATP transport, phosphatidylinositol-phospha-tidylcholine transfer protein function and multiple-drug sensitivity (3). Sac1p is an important regulator of microsomal ATP transport, providing a link between inositol phospholipid signaling and ATP-dependent processes in the yeast ER (4). Defects in Sac1p relieves the requirement for Sec14p by altering phospholipid metabolism to expand the pool of diacylglycerol in the Golgi (5). Sac1p dysfunction exerts its pleiotropic effects on yeast Golgi function, the organization of the actin cytoskeleton, and the cellular requirement for inositol, through altered metabolism of inositol glycerophospholipids (6). These effects suggest the secretory and cytoskeletal activities are coordinated to achieve proper spatial regulation of secretion in S. cerevisiae (7). |
| 应用 | 推荐稀释比例 |
| {ICC/IF} | {1:50-200} |
| {IF} | {1:100-500} |
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文献和实验Fluorochrome Absorption and Emission Spectra
. Due to its photobleaching characteristics, PerCP conjugates are not recommended for use on stream-in-air flow cytometers. PerCP-Cy5.5 is a tandem conjugate system than combines PerCP with a cyanine dye (Cy5.5™) and has an absorption maximum
Fluorochrome Absorption and Emission
. Due to its photobleaching characteristics, PerCP conjugates are not recommended for use on stream-in-air flow cytometers. PerCP-Cy5.5 is a tandem conjugate system than combines PerCP with a cyanine dye (Cy5.5™) and has an absorption maximum
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