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产品信息以Bioss网站为准
- 规格:
100ul
| 产品编号 | bs-13480R-HRP |
| 英文名称 | GOLGA3 Rabbit pAb, HRP conjugated |
| 中文名称 | HRP标记的高尔基体膜相关蛋白抗体 |
| 英文别名 | GCP170; GOLGA 3; GOLGA3; Golgi autoantigen; Golgi complex associated protein of 170 kDa; Golgi complex-associated protein of 170 kD; Golgi membrane associated protein; Golgi peripheral membrane protein; Golgin 160; Golgin subfamily a 3; HGNC:4426; Male enhanced antigen 2; MEA2; SY2/SY10 protein; GOGA3_HUMAN. |
| 产品应用 | WB=1000-10000, IHC-P=1:100-500, IHC-F=1:100-500, ELISA=1:500-5000 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GOLGA3/Golgin 160 |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Protein Trafficking > Golgi Proteins |
| 亚基 | Homodimer. Interacts with GOLGA7. Isoform 1 interacts with GOPC while isoform 3 does not. |
| 亚细胞定位 | Cytoplasmic and Golgi Apparatus. |
| 组织特异性 | Expressed in all tissues tested. Expressed in liver, testis, lung, heart, salivary gland and kidney. |
| 翻译后修饰 | Cleaved by caspases in apoptotic cells. |
| 功能 | GOLGA3 is a member of the golgin family of proteins which are localized to the Golgi. It has been postulated to play a role in nuclear transport and Golgi apparatus localization. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 背景资料 | The Golgi apparatus consists of a series of stacked, flattened membrane stacks called cisternae that are involved in the transport of lipids and proteins in the secretory pathway and are important for Golgi-microtubule interaction. Golgin 160 is a 1,498 amino acid protein that localizes to both the cytoplasm and to the Golgi apparatus and contains a series of coiled-coil domains. Expressed in a variety of tissues, including heart, liver, testis, kidney, lung and salivary gland, golgin 160 functions as a homodimer that interacts with GOLGA7 and is thought to be involved in maintaining Golgi structure and may play a role in nuclear transport and Golgi apparatus localization. Multiple isoforms of golgin 160 exist due to alternative splicing events. |
| 应用 | 推荐稀释比例 |
| {WB} | {1000-10000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ELISA} | {1:500-5000} |
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文献和实验affinity chromatography and conjugated with horseradish peroxidase (HRP). Formulation Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) (pH 7.0) with stabilizers. Storage Neat samples can be stored at 2° - 8° C
Western Immunoblotting of Proteins
. For a mouse monoclonal antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes
Western Immunoblotting of Proteins
antibody, a goat-anti-mouse HRP conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat-anti-rabbit HRP conjugated antibody is advisable. 7. Wash the nitrocellulose membrane five times for 3 to 5 minutes each with either water
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