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- 文献和实验
- 技术资料
- 保存条件:
储存条件:-20℃,干燥、避光、密封
- 保质期:
2年
- 英文名:
alpha-CEHC/α-CEHC
- 库存:
1000
- 供应商:
四川省维克奇生物科技有限公司
- CAS号:
4072-32-6
- 规格:
1mg
英文名称:alpha-CEHC/α-CEHC
CAS:4072-32-6
储存条件:-20℃,干燥、避光、密封
规格:5mg10mg20mg50mg100mg500mg1g80g等应客户需求包装
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文献和实验(29:1 ratio; 40% stock solution) (Sigma) (for hot-stop PCR only; see Steps 1-22)Agarose (for duplex PCR only; see Steps 40-53)Ammonium persulfate (APS; 10%, w/v), freshly prepared (for hot-stop PCR [Steps 1-22] or SSCP [Steps 23-37] only)α-32PdCTP (10
, and Klenow fragment exo(−) (Kf−). Primer was 5′‐end‐labeled using [γ‐32 P]ATP and polynucleotide kinase. Polymerization reactions were carried out with primer (3.5 µM), template (5 µM), all dNTPs and dNTPαS (0.1 mM each, final concentration), and Kf− (0.015 µ
In Vitro Transcription Assay (Run-off Assay) using Permeabil
in 3 to 7 days. F. TCA Precipitation to Determine Incorporation of [32 P] GTP into Nucleic Acid (see Hint #8) 1. Add 1 μl aliquot of reaction assay to a 13 x 100 mm tube containing 2 ml of Yeast tRNA Solution. 2. Add 1 ml of 10% TCA. Mix. 3. Incubate on ice
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