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- 文献和实验
- 技术资料
- 保存条件:
储存条件:-20℃,干燥、避光、密封
- 保质期:
2年
- 英文名:
(1-OXYL-2,2,5,5-TETRAMETHYL-3-PYRROLINE)FORMALDEHYDE
- 库存:
1000
- 供应商:
四川省维克奇生物科技有限公司
- CAS号:
71051-83-7
- 规格:
10mg
英文名称:(1-OXYL-2,2,5,5-TETRAMETHYL-3-PYRROLINE)FORMALDEHYDE
分子式:C9H14NO2
分子量:168.21
CAS:71051-83-7
熔点:62-64°C
储存条件:-20℃,干燥、避光、密封
规格:5mg10mg20mg50mg100mg500mg1g80g等应客户需求包装
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文献和实验7.5,83 ml nuclease-free water •Protease inhibitors (200mM PMSF in isopropanol,2 mg/ml Aprotinin,2 mg/ml Leupeptin)•37% reagent grade Formaldehyde•1.25 M glycine•1% Agarose gel/EtBr1.Transfect,induce,or grow 1 to 5x106 cells in 10 cm dish.2.Rinse cells
Chromatin IP (CHIP assay) This protocol has some minor modification to the protocol described in Strahl-Bolsinger S. et al. [1997, Gen & Dev 11, p83-93] and was obtained from Flick K. (The Scripps Research Institute).
DNA Amplification Fingerprinting Protocol
mL of 10X TBE buffer (1M Tris. HCl, 0.83 M boric acid, 10 mM Na2 EDTA, pH 8.3). Swirl to mix. Add 60 µL of 10% (w/v) ammonium persulfate and 5 µL of TEMED. Mix thoroughly. Immediately pour the gel mixture into the gel cassette (Mini
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