- ￥2000 - 8000
- 湖北省武汉市
- Pseudomonas putida crnA 重组蛋白表达
- 2025年11月24日
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- 详细信息
- 文献和实验
- 技术资料
- 服务名称：
大肠杆菌系统重组蛋白表达
- 提供商：
普健生物（武汉）科技有限公司
Uniprot号：P83772
基因名：crnA
蛋白名：Creatinine amidohydrolase
蛋白别名：
Creatininase
服务内容：
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 |
基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示：
P83772相关研究文献：
1. Cloning of the creatinine amidohydrolase gene from Pseudomonas sp. PS-7.
2. Creatinine amidohydrolase (creatininase) from Pseudomonas putida. Purification and some properties.
3. Crystal structure of creatininase from Pseudomonas putida: a novel fold and a case of convergent evolution.
4. Crystal structures of creatininase reveal the substrate binding site and provide an insight into the catalytic mechanism.
5. Substitution of Glu122 by glutamine revealed the function of the second water molecule as a proton donor in the binuclear metal enzyme creatininase.
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文献和实验Electrotransformation of Pseudomonas
of this family is Pseudomonas aeruginosa (1 ). Pseudomonas species have previously been reported to be transformed genetically by conjugation, transfection, or chemical transformation. In addition, several investigators have described the electroporation of P
Here, we document a technique to reduce the size of the genome of Pseudomonas putida by using a combinatorial mini-Tn5 -targeted Flp-FRT recombination system. This method combines random insertions with the site-specific Flp-FRT recombination
) into a measurable readout typically fluorescence or luminescence. Various biosensors for BTEX (a mixture of benzene, toluene, ethylbenzene and xylenes) are based on XylR, the main transcriptional regulator of the TOL pathway of Pseudomonas putida mt
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