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- TIANDZ
- ZK-0115604
- 中国
- 2025年11月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
pAG426GAL-ccdB-HA(pAG426GALccdBHA)
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号:
质粒名称: pAG426GAL-ccdB-HA
其他名称:
质粒大小（bp）: 8223
抗性: Ampicillin
质粒类型: Yeast Expression
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性:
质粒图谱:
质粒序列:
>Author sequence
gacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagtatga
tccaatatcaaaggaaatgatagcattgaaggatgagactaatccaattgaggagtggcagcatatagaa
cagctaaagggtagtgctgaaggaagcatacgataccccgcatggaatgggataatatcacaggaggtac
tagactacctttcatcctacataaatagacgcatataagtacgcatttaagcataaacacgcactatgcc
gttcttctcatgtatatatatatacaggcaacacgcagatataggtgcgacgtgaacagtgagctgtatg
tgcgcagctcgcgttgcattttcggaagcgctcgttttcggaaacgctttgaagttcctattccgaagtt
cctattctctagaaagtataggaacttcagagcgcttttgaaaaccaaaagcgctctgaagacgcacttt
caaaaaaccaaaaacgcaccggactgtaacgagctactaaaatattgcgaataccgcttccacaaacatt
gctcaaaagtatctctttgctatatatctctgtgctatatccctatataacctacccatccacctttcgc
tccttgaacttgcatctaaactcgacctctacattttttatgtttatctctagtattactctttagacaa
aaaaattgtagtaagaactattcatagagtgaatcgaaaacaatacgaaaatgtaaacatttcctatacg
tagtatatagagacaaaatagaagaaaccgttcataattttctgaccaatgaagaatcatcaacgctatc
actttctgttcacaaagtatgcgcaatccacatcggtatagaatataatcggggatgcctttatcttgaa
aaaatgcacccgcagcttcgctagtaatcagtaaacgcgggaagtggagtcaggctttttttatggaaga
gaaaatagacaccaaagtagccttcttctaaccttaacggacctacagtgcaaaaagttatcaagagact
gcattatagagcgcacaaaggagaaaaaaagtaatctaagatgctttgttagaaaaatagcgctctcggg
atgcatttttgtagaacaaaaaagaagtatagattctttgttggtaaaatagcgctctcgcgttgcattt
ctgttctgtaaaaatgcagctcagattctttgtttgaaaaattagcgctctcgcgttgcatttttgtttt
acaaaaatgaagcacagattcttcgttggtaaaatagcgctttcgcgttgcatttctgttctgtaaaaat
gcagctcagattctttgtttgaaaaattagcgctctcgcgttgcatttttgttctacaaaatgaagcaca
gatgcttcgttcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaata
cattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaaga
gtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgc
tcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaa
ctggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcactt
ttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcat
acactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgaca
gtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacga
tcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttg
ggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaaca
acgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatgg
aggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatc
tggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatc
gtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtg
cctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaact
tcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgt
gagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttc
tgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaaga
gctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtg
tagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgt
taccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccgga
taaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacacc
gaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggt
atccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatct
ttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcgg
agcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcaca
tgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgc
tcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaa
ccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgg
gcagtgagcgcaacgcaattaatgtgagttacctcactcattaggcaccccaggctttacactttatgct
tccggctcctatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgat
tacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctctagtacggattagaagccg
ccgagcgggcgacagccctccgacggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcct
gaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggtt
atgaagaggaaaaattggcagtaacctggccccacaaaccttcaaattaacgaatcaaattaacaaccat
aggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttga
tctattaacagatatataaatggaaaagctgcataaccactttaactaatactttcaacattttcagttt
gtattacttcttattcaaatgtcataaaagtatcaacaaaaaattgttaatatacctctatactttaacg
tcaaggagaaaaaaccccggattctagaactagtggatcccccatcacaagtttgtacaaaaaagctgaa
cgagaaacgtaaaatgatataaatatcaatatattaaattagattttgcataaaaaacagactacataat
actgtaaaacacaacatatccagtcactatggcggccgcattaggcaccccaggctttacactttatgct
tccggctcgtataatgtgtggattttgagttaggatccgtcgagattttcaggagctaaggaagctaaaa
tggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggc
atttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagacc
gtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatc
cggaattccgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgt
tttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttcta
cacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgaga
atatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatgga
caacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctg
gcgattcaggttcatcatgccgtctgtgatggcttccatgtcggcagaatgcttaatgaattacaacagt
actgcgatgagtggcagggcggggcgtaaacgccgcgtggatccggcttactaaaagccagataacagta
tgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaa
agaggtatgctatgaagcagcgtattacagtgacagttgacagcgacagctatcagttgctcaaggcata
tatgatgtcaatatctccggtctggtaagcacaaccatgcagaatgaagcccgtcgtctgcgtgccgaac
gctggaaagcggaaaatcaggaagggatggctgaggtcgcccggtttattgaaatgaacggctcttttgc
tgacgagaacaggggctggtgaaatgcagtttaaggtttacacctataaaagagagagccgttatcgtct
gtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgca
cgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgca
tgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccg
cgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataaatgtcaggctcccttatacac
agccagtctgcaggtcgaccatagtgactggatatgttgtgttttacagtattatgtagtctgtttttta
tgcaaaatctaatttaatatattgatatttatatcattttacgtttctcgttcagctttcttgtacaaag
tggtgatgggctgcaggaattcgatatcaagcttaggtggaatgtacccatacgatgttcctgactatgc
gggctatccctatgacgtcccggactatgcaggatcctatccatatgacgttccagattacgctgctcag
tgctaactcgagtcatgtaattagttatgtcacgcttacattcacgccctccccccacatccgctctaac
cgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtatta
agaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatac
tgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgcggccggtacccaattcgccc
tatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcg
ttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcac
cgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgacgcgccctgtagcggcgcatta
agcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctt
tcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccc
tttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgt
agtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggac
tcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgcc
gatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatatta
acgtttacaatttcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatagggta
ataactgatataattaaattgaagctctaatttgtgagtttagtatacatgcatttacttataatacagt
tttttagttttgctggccgcatcttctcaaatatgcttcccagcctgcttttctgtaacgttcaccctct
accttagcatcccttccctttgcaaatagtcctcttccaacaataataatgtcagatcctgtagagacca
catcatccacggttctatactgttgacccaatgcgtctcccttgtcatctaaacccacaccgggtgtcat
aatcaaccaatcgtaaccttcatctcttccacccatgtctctttgagcaataaagccgataacaaaatct
ttgtcgctcttcgcaatgtcaacagtacccttagtatattctccagtagatagggagcccttgcatgaca
attctgctaacatcaaaaggcctctaggttcctttgttacttcttctgccgcctgcttcaaaccgctaac
aatacctgggcccaccacaccgtgtgcattcgtaatgtctgcccattctgctattctgtatacacccgca
gagtactgcaatttgactgtattaccaatgtcagcaaattttctgtcttcgaagagtaaaaaattgtact
tggcggataatgcctttagcggcttaactgtgccctccatggaaaaatcagtcaagatatccacatgtgt
ttttagtaaacaaattttgggacctaatgcttcaactaactccagtaattccttggtggtacgaacatcc
aatgaagcacacaagtttgtttgcttttcgtgcatgatattaaatagcttggcagcaacaggactaggat
gagtagcagcacgttccttatatgtagctttcgacatgatttatcttcgtttcctgcaggtttttgttct
gtgcagttgggttaagaatactgggcaatttcatgtttcttcaacactacatatgcgtatatataccaat
ctaagtctgtgctccttccttcgttcttccttctgttcggagattaccgaatcaaaaaaatttcaaagaa
accgaaatcaaaaaaaagaataaaaaaaaaatgatgaattgaattgaaaagctgtggtatggtgcactct
cagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccc
tgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtc
agaggttttcaccgtcatcaccgaaacgcgcga
>14251 with pRS_marker
TTCATCATTTTTTTTTTATTCTTTTTTTTGATTTCGGTTTCCTTGAAATTTTTTTGATTCGGTAATCTCC
GAACAGAAGGAAGAACGAAGGAAGGAGCACAGACTTAGATTGGTATATATACGCATATGTAGTGTTGAAG
AAACATGAAATTGCCCAGTATTCTTAACCCAACTGCACAGAACAAAAACCTGCAGGAAACGAAGATAAAT
CATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAAT
ATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGT
TAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCAT
GGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAA
TTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAG
ACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGT
AACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAA
TATACTAAGGGTACTGTTG
>14251 with GW5
TAAAGTGTAAAGCCTGGGGGTGCCTAATGCGGCCGCCATAGTGACTGGATATGTTGTGTTTTACAGTATT
ATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTC
AGCTTTTTTGTACAAACTTGTGATGGGGGATCCACTAGTTCTAGAATCCGGGGTTTTTTCTCCTTGACGT
TAAAGTATAGAGGTATATTAACAATTTTTTGTTGATACTTTTATTACATTTGAATAAGAAGTAATACAAA
CCGAAAATGTTGAAAGTATTAGTTAAAGTGGTTATGCAGTTTTTGCATTTATATATCTGTTAATAGATCA
AAAATCATCGCTTCGCTGATTAATTACCCCAGAAATAAGGCTAAAAAACTAATCGCATTATCATCCTATG
GTTGTTAATTTGATTCGTTCATTTGAAGGTTTGTGGGGCCAGGTTACTGCCAATTTTTCCTCTTCATAAC
CATAAAAGCTAGTATTGTAGAATCTTTATTGTTCGGAGCAGTGCGGCGCGAGGCACATCTGCGTTTCAGG
AACGCGACCGGTGAAGACGAGGACGCACGGAGGAGAGTCTTCCTTCGGAGGGCTGTCACCCGCTCGGCGG
CTTCTAATCCGTACTAGAGCTCCAGCTTTTGTTCCCTTT
>14251 with GW3
CGTTATCGGGGGAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATG
TTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATA
TGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATAT
CATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATGGGCTGCAGGAATTCGATATCAAGCTT
AGGTGGAATGTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGA
TCCTATCCATATGACGTTCCAGATTACGCTGCTCAGTGCTAACTCGAGTCATGTAATTAGTTATGTCACG
CTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAG
GTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTT
TTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTC
GAAGGCTTTAATTTGCGGCCGGTACCCAATTCGCCCTATAGTGAGTCGTATTACGCGCGCTCA
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文献和实验and requires induction of the GAL1-10 promoter on galactose. Our strains grow poorly on galactose but give 80 to 100% popouts. The HA tag can be detected by mouse monoclonal antibodies 12CA5 (Boehringer) or MMS101R (BAbCo, Richmond, California
insertion. The popout event is mediated by cre recombinase and requires induction of the GAL1-10 promoter on galactose. Our strains grow poorly on galactose but give 80 to 100% popouts. The HA tag can be detected by mouse monoclonal antibodies 12CA
Interaction Trap/Two‐Hybrid System to Identify Interacting Proteins
4.4.2 ) 100‐mm complete minimal (CM) medium dropout plates (Treco and Lundblad, ) with 2% (w/v) glucose (Glu) or 2% (w/v) galactose (Gal
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