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- 详细信息
- 文献和实验
- 技术资料
- 克隆性:
Monoclonal
- 抗体英文名:
Bacteroides thetaiotaomicron antibody [B134M]
- 抗体名:
Bacteroides thetaiotaomicron antibody [B134M]
- 宿主:
Mouse
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
ELISA,
- 浓度:
1mg/1ml
- 保存条件:
-20℃
- 规格:
100ug
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文献和实验This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography.1. Solutions(1) Affigel Blue Prewash0.1 M acetic acid 5.7 ml glacial acetic acid1.4 M NaCl 81 g NaCl40% isopropanol 400 ml isopropanolup
Antibody Purification This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography. Solutions Affigel Blue Prewash 0.1 M acetic acid 5.7 ml glacial acetic acid
Antibody Staining of Ovaries 1. Dissect ovaries in EBR 2. Transfer ovaries into an Eppendorf tube and add 100 µl devitellinizing buffer and 600-µl heptane 3. Agitate gently for 10 minutes
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