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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
2×RealStar Probe Fast Mixture UNG(with ROX II)
- 保质期:
一年
- 供应商:
上海再康生物科技有限公司
- 保存条件:
常温或冰箱保存
- 规格:
1.1 ml/1.1 mlx 5/1.1 mlx 10
【产品概述】
|
产品名称
|
货号
|
规格
|
|
2×RealStar Probe Fast Mixture UNG
|
ZK352-01
|
1.1 ml
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ZK352-05
|
1.1 ml x 5
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|
|
ZK352-10
|
1.1 ml x 10
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|
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2×RealStar Probe Fast Mixture UNG with ROX
|
ZK355-01
|
1.1 ml
|
|
ZK355-05
|
1.1 ml x 5
|
|
|
ZK355-10
|
1.1 ml x 10
|
|
|
2×RealStar Probe Fast Mixture UNG with ROX II
|
ZK356-01
|
1.1 ml
|
|
ZK356-05
|
1.1 ml x 5
|
|
|
ZK356-10
|
1.1 ml x 10
|
ZK355: ABI Prism7000/7300/7700/7900HT和ABI Step One /ABI Step One Plus荧光定量PCR仪。
ZK356: ABI Prism7500 /7500 Fast荧光定量PCR仪 , MJ Research Chromo4,Opticon (II) , Corbett Rotor Gene 3000 , Agilent Technologies Mx3000P荧光定量PCR仪。
ZK352: Thermal Cycler Dice Real Time System , LightCycler , Smart Cycler System,Corbett Rotor-gene 6000荧光定量PCR仪等。
| 货号 | 规格 | 备注 | |
| ROX Reference Dye ROX 参考荧光染料 |
ZK320-01 | 40 μl | 1 ml qPCRMix选送 |
| ZK320-05 | 200 μl | 5 ml qPCRMix 选送 | |
| ZK320-10 | 400 μl | 10 ml qPCRMix 选送 | |
| ROX Reference Dye II ROX 参考荧光染料 II |
ZK321-01 | 40 μl | 1 ml qPCRMix选送 |
| ZK321-05 | 200 μl | 5 ml qPCRMix 选送 | |
| ZK321-10 | 400 μl | 10 ml qPCRMix 选送 |
【注意事项】
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文献和实验Screening lambda cDNA or genomic libraries
ZAP II.For lambda gt11 use the host Y1090.The Barstead mixed stage C.elegans cDNA library is in the original lamda ZAP vector,and requires a host carrying the supF mutation,such as Y1090.Growing the cells:grow up 40 ml of bacteria o/n in LBspin 5 min
.1 RNA Probe Preparation (see Note 1 ) 1. 1.5 mL microcentrifuge tubes or standard 96-well V-bottom microplates. 2. RNAse free water. 3. T7, T3 or SP6 RNA Polymerase (Fermentas Life Sciences, Burlington, ON, Canada
RNA Analysis by Nuclease Protection
g) was hybridized in replicate reactions to a probe for an AT‐rich cyclin gene. Reactions were treated with either RNase T1, RNase A, RNase A/RNase T1 mixture, or RNase I (all from E. coli ), using two‐fold different amounts of each RNase. AU‐rich
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