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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Betaine, 5 M
- 保质期:
-20℃恒温保存两年,经常使用建议适量分装;避免反复冻融
- 供应商:
上海再康生物科技有限公司
- 保存条件:
两年
- 规格:
1 ml
本产品为高纯度Betaine制备的5 M溶液,经严格灭菌处理,适用于以高GC含量的DNA为模板时作为添加剂优化反应条件。其原理可能是降低高GC模板的变性温度,从而减少二级结构的形成。Betaine的最佳终浓度通常为0.8~1.6 M,针对不同反应需摸索其最佳浓度。
保存条件
-20℃恒温保存两年,经常使用建议适量分装;避免反复冻融
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文献和实验Strategy for accurate sequencing through hairpin structures
as an abrupt stop in the sequence peak profile for pHSPG-shTLR4 (Figure 3A). Among the DNA relaxing agents, 5% DMSO, 0.83 M Betaine and 1 × PCRx Enhancer each improved the sequence read significantly for some constructs. However,the addition of 0.83 M Betaine
) and that provided by CLONTECH in the Advantage-GC cDNA PCR kit is in fact Betaine which is available at a fraction of the cost as a 5M solution from Sigma-Aldrich (cat. # B 0300), but be sure to use Betaine or Betaine (mono)hydrate and not Betaine HCl
An Economic PCR Enhancer for GC-Rich PCR Templates
several different polymerases The 5x concentrated Combinatorial Enhancer Solution (CES) contains: 2.7 M betaine 6.7 mM DTT 6.7% DMSO and 55 μg/ml BSA Add this solution to your PCR and see if it works Some hints if your PCR
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