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- 2025年11月21日
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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
pET-41a(+)
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号: 60908-2807y
质粒名称: pET-41a(+)
其他名称:
质粒大小（bp）: 5933
抗性: Kanamycin （卡那霉素）
质粒类型: 大肠杆菌蛋白表达
是否病毒: 非病毒
启动子:
稳转或瞬转: 瞬时表达
组成型或诱导性: 组成型
表达水平: 高
蛋白标签: N-GST, N-His, N-Thrombin, N-EK, N-S
5′ 测序引物及序列:pGEX-5′: 5′-GGGCTGGCAAGCCACGTTTGGTG-3′
3′ 测序引物及序列:T7t: 5′-GCTAGTTATTGCTCAGCGG-3′
GenBank号:
其他属性: Encodes GST fusion tag; Nterm thrombin cleavage site; Nterm enterokinase cleavage site; PshAI blunt cloning site; a,b,c vary by MCS.
质粒图谱:
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 120 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| EK | 279 | 265 | |
| S15 | 354 | 310 | |
| 6xHIS | 414 | 397 | |
| GST (variant) | 1095 | 445 | |
| T7_transl_en_RBS | 1120 | 1104 | |
| lacO | 1165 | 1138 | |
| T7_promoter | 1183 | 1165 | |
| tet (300 – 563) | 1219 | 1482 | |
| pBRrevBam_primer | 1290 | 1271 | |
| lacI | 1565 | 2656 | |
| ROP | 3228 | 3419 | |
| pGEX_3_primer | 3435 | 3413 | |
| pBR322_origin | 4453 | 3834 | |
| KanR2 | 4559 | 5374 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 3 | 1095 | 139 | |
| ORF frame 1 | 1096 | 1824 | |
| ORF frame 2 | 1697 | 2656 | |
| ORF frame 2 | 4559 | 5374 |
| Enzyme Name | Cut |
|---|---|
| XhoI | 174 |
| NotI | 182 |
| EagI | 182 |
| HindIII | 189 |
| SalI | 195 |
| SacI | 206 |
| PstI | 214 |
| AscI | 216 |
| StuI | 226 |
| EcoRI | 235 |
| BamHI | 241 |
| EcoRV | 251 |
| NcoI | 255 |
| AgeI | 292 |
| KpnI | 299 |
| BglII | 302 |
| SacII | 393 |
| SpeI | 424 |
| MscI | 887 |
| NdeI | 1094 |
| XbaI | 1132 |
| ApaI | 2135 |
| HpaI | 2430 |
| NruI | 4647 |
| SmaI | 4864 |
| XmaI | 4862 |
质粒序列:
| 1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
| 51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
| 101 | ctcagcttcc | tttcgggctt | tgtttagcag | cctaggtatt | aatcaattag | 150 |
| 151 | tggtggtggt | ggtggtggtg | gtgctcgagt | gcggccgcaa | gcttgtcgac | 200 |
| 201 | ggagctcgcc | tgcaggcgcg | ccaaggcctg | tacagaattc | ggatccccga | 250 |
| 251 | tatcccatgg | gactcttgtc | gtcgtcatca | ccggagccac | caccggtacc | 300 |
| 301 | cagatctggg | ctgtccatgt | gctggcgttc | gaatttagca | gcagcggttt | 350 |
| 351 | ctttcatacc | aattgcagta | ctaccgcgtg | gcaccagacc | cgcggagtga | 400 |
| 401 | tggtgatggt | gatgaccaga | accactagtt | gaaccatccg | attttggagg | 450 |
| 451 | atggtcgcca | ccaccaaacg | tggcttgcca | gccctgcaaa | ggccatgcta | 500 |
| 501 | tatacttgct | ggatttcaag | tacttatcaa | tttgtgggat | agcttcaata | 550 |
| 551 | cgttttttaa | aacaaactaa | ttttgggaac | gcatccaggc | acattgggtc | 600 |
| 601 | catgtataaa | acaacatcaa | gagcgtcata | caacatgaag | tcaggatggg | 650 |
| 651 | ttacatgatc | accatttaaa | tatgttttat | gacataaacg | atcttcgaac | 700 |
| 701 | attttcagca | tttcaggtag | cttgctaaga | aaatcaactt | tgagagtttc | 750 |
| 751 | aaagtcttta | ctatatgcaa | ttctcgaaac | accgtatcta | atatccaaaa | 800 |
| 801 | ccgctccttc | aagcattgaa | atctctgcac | gctcttttgg | acaaccaccc | 850 |
| 851 | aacatgttgt | gcttgtcagc | tatataacgt | atgatggcca | tagactgtgt | 900 |
| 901 | taatttaaca | tcaccatcaa | tataataagg | aagattggga | aactccaaac | 950 |
| 951 | ccaattcaaa | ctttttgttt | cgccatttat | caccttcatc | gcgctcatac | 1000 |
| 1001 | aaatgctctt | catatttttc | ttcaagatat | tccaaaagaa | gtcgagtggg | 1050 |
| 1051 | ttgcacaagg | cccttaattt | tccaataacc | tagtataggg | gacatatgta | 1100 |
| 1101 | tatctccttc | ttaaagttaa | acaaaattat | ttctagaggg | gaattgttat | 1150 |
| 1151 | ccgctcacaa | ttcccctata | gtgagtcgta | ttaatttcgc | gggatcgaga | 1200 |
| 1201 | tcgatctcga | tcctctacgc | cggacgcatc | gtggccggca | tcaccggcgc | 1250 |
| 1251 | cacaggtgcg | gttgctggcg | cctatatcgc | cgacatcacc | gatggggaag | 1300 |
| 1301 | atcgggctcg | ccacttcggg | ctcatgagcg | cttgtttcgg | cgtgggtatg | 1350 |
| 1351 | gtggcaggcc | ccgtggccgg | gggactgttg | ggcgccatct | ccttgcatgc | 1400 |
| 1401 | accattcctt | gcggcggcgg | tgctcaacgg | cctcaaccta | ctactgggct | 1450 |
| 1451 | gcttcctaat | gcaggagtcg | cataagggag | agcgtcgaga | tcccggacac | 1500 |
| 1501 | catcgaatgg | cgcaaaacct | ttcgcggtat | ggcatgatag | cgcccggaag | 1550 |
| 1551 | agagtcaatt | cagggtggtg | aatgtgaaac | cagtaacgtt | atacgatgtc | 1600 |
| 1601 | gcagagtatg | ccggtgtctc | ttatcagacc | gtttcccgcg | tggtgaacca | 1650 |
| 1651 | ggccagccac | gtttctgcga | aaacgcggga | aaaagtggaa | gcggcgatgg | 1700 |
| 1701 | cggagctgaa | ttacattccc | aaccgcgtgg | cacaacaact | ggcgggcaaa | 1750 |
| 1751 | cagtcgttgc | tgattggcgt | tgccacctcc | agtctggccc | tgcacgcgcc | 1800 |
| 1801 | gtcgcaaatt | gtcgcggcga | ttaaatctcg | cgccgatcaa | ctgggtgcca | 1850 |
| 1851 | gcgtggtggt | gtcgatggta | gaacgaagcg | gcgtcgaagc | ctgtaaagcg | 1900 |
| 1901 | gcggtgcaca | atcttctcgc | gcaacgcgtc | agtgggctga | tcattaacta | 1950 |
| 1951 | tccgctggat | gaccaggatg | ccattgctgt | ggaagctgcc | tgcactaatg | 2000 |
| 2001 | ttccggcgtt | atttcttgat | gtctctgacc | agacacccat | caacagtatt | 2050 |
| 2051 | attttctccc | atgaagacgg | tacgcgactg | ggcgtggagc | atctggtcgc | 2100 |
| 2101 | attgggtcac | cagcaaatcg | cgctgttagc | gggcccatta | agttctgtct | 2150 |
| 2151 | cggcgcgtct | gcgtctggct | ggctggcata | aatatctcac | tcgcaatcaa | 2200 |
| 2201 | attcagccga | tagcggaacg | ggaaggcgac | tggagtgcca | tgtccggttt | 2250 |
| 2251 | tcaacaaacc | atgcaaatgc | tgaatgaggg | catcgttccc | actgcgatgc | 2300 |
| 2301 | tggttgccaa | cgatcagatg | gcgctgggcg | caatgcgcgc | cattaccgag | 2350 |
| 2351 | tccgggctgc | gcgttggtgc | ggacatctcg | gtagtgggat | acgacgatac | 2400 |
| 2401 | cgaagacagc | tcatgttata | tcccgccgtt | aaccaccatc | aaacaggatt | 2450 |
| 2451 | ttcgcctgct | ggggcaaacc | agcgtggacc | gcttgctgca | actctctcag | 2500 |
| 2501 | ggccaggcgg | tgaagggcaa | tcagctgttg | cccgtctcac | tggtgaaaag | 2550 |
| 2551 | aaaaaccacc | ctggcgccca | atacgcaaac | cgcctctccc | cgcgcgttgg | 2600 |
| 2601 | ccgattcatt | aatgcagctg | gcacgacagg | tttcccgact | ggaaagcggg | 2650 |
| 2651 | cagtgagcgc | aacgcaatta | atgtaagtta | gctcactcat | taggcaccgg | 2700 |
| 2701 | gatctcgacc | gatgcccttg | agagccttca | acccagtcag | ctccttccgg | 2750 |
| 2751 | tgggcgcggg | gcatgactag | catgatcgtg | ctcctgtcgt | tgaggacccg | 2800 |
| 2801 | gctaggctgg | cggggttgcc | ttactggtta | gcagaatgaa | tcaccgatac | 2850 |
| 2851 | gcgagcgaac | gtgaagcgac | tgctgctgca | aaacgtctgc | gacctgagca | 2900 |
| 2901 | acaacatgaa | tggtcttcgg | tttccgtgtt | tcgtaaagtc | tggaaacgcg | 2950 |
| 2951 | gaagtcagcg | ccctgcacca | ttatgttccg | gatctgcatc | gcaggatgct | 3000 |
| 3001 | gctggctacc | ctgtggaaca | cctacatctg | tattaacgaa | gcgctggcat | 3050 |
| 3051 | tgaccctgag | tgatttttct | ctggtcccgc | cgcatccata | ccgccagttg | 3100 |
| 3101 | tttaccctca | caacgttcca | gtaaccgggc | atgttcatca | tcagtaaccc | 3150 |
| 3151 | gtatcgtgag | catcctctct | cgtttcatcg | gtatcattac | ccccatgaac | 3200 |
| 3201 | agaaatcccc | cttacacgga | ggcatcagtg | accaaacagg | aaaaaaccgc | 3250 |
| 3251 | ccttaacatg | gcccgcttta | tcagaagcca | gacattaacg | cttctggaga | 3300 |
| 3301 | aactcaacga | gctggacgcg | gatgaacagg | cagacatctg | tgaatcgctt | 3350 |
| 3351 | cacgaccacg | ctgatgagct | ttaccgcagc | tgcctcgcgc | gtttcggtga | 3400 |
| 3401 | tgacggtgaa | aacctctgac | acatgcagct | cccggagacg | gtcacagctt | 3450 |
| 3451 | gtctgtaagc | ggatgccggg | agcagacaag | cccgtcaggg | cgcgtcagcg | 3500 |
| 3501 | ggtgttggcg | ggtgtcgggg | cgcagccatg | acccagtcac | gtagcgatag | 3550 |
| 3551 | cggagtgtat | actggcttaa | ctatgcggca | tcagagcaga | ttgtactgag | 3600 |
| 3601 | agtgcaccat | atatgcggtg | tgaaataccg | cacagatgcg | taaggagaaa | 3650 |
| 3651 | ataccgcatc | aggcgctctt | ccgcttcctc | gctcactgac | tcgctgcgct | 3700 |
| 3701 | cggtcgttcg | gctgcggcga | gcggtatcag | ctcactcaaa | ggcggtaata | 3750 |
| 3751 | cggttatcca | cagaatcagg | ggataacgca | ggaaagaaca | tgtgagcaaa | 3800 |
| 3801 | aggccagcaa | aaggccagga | accgtaaaaa | ggccgcgttg | ctggcgtttt | 3850 |
| 3851 | tccataggct | ccgcccccct | gacgagcatc | acaaaaatcg | acgctcaagt | 3900 |
| 3901 | cagaggtggc | gaaacccgac | aggactataa | agataccagg | cgtttccccc | 3950 |
| 3951 | tggaagctcc | ctcgtgcgct | ctcctgttcc | gaccctgccg | cttaccggat | 4000 |
| 4001 | acctgtccgc | ctttctccct | tcgggaagcg | tggcgctttc | tcatagctca | 4050 |
| 4051 | cgctgtaggt | atctcagttc | ggtgtaggtc | gttcgctcca | agctgggctg | 4100 |
| 4101 | tgtgcacgaa | ccccccgttc | agcccgaccg | ctgcgcctta | tccggtaact | 4150 |
| 4151 | atcgtcttga | gtccaacccg | gtaagacacg | acttatcgcc | actggcagca | 4200 |
| 4201 | gccactggta | acaggattag | cagagcgagg | tatgtaggcg | gtgctacaga | 4250 |
| 4251 | gttcttgaag | tggtggccta | actacggcta | cactagaagg | acagtatttg | 4300 |
| 4301 | gtatctgcgc | tctgctgaag | ccagttacct | tcggaaaaag | agttggtagc | 4350 |
| 4351 | tcttgatccg | gcaaacaaac | caccgctggt | agcggtggtt | tttttgtttg | 4400 |
| 4401 | caagcagcag | attacgcgca | gaaaaaaagg | atctcaagaa | gatcctttga | 4450 |
| 4451 | tcttttctac | ggggtctgac | gctcagtgga | acgaaaactc | acgttaaggg | 4500 |
| 4501 | attttggtca | tgaacaataa | aactgtctgc | ttacataaac | agtaatacaa | 4550 |
| 4551 | ggggtgttat | gagccatatt | caacgggaaa | cgtcttgctc | taggccgcga | 4600 |
| 4601 | ttaaattcca | acatggatgc | tgatttatat | gggtataaat | gggctcgcga | 4650 |
| 4651 | taatgtcggg | caatcaggtg | cgacaatcta | tcgattgtat | gggaagcccg | 4700 |
| 4701 | atgcgccaga | gttgtttctg | aaacatggca | aaggtagcgt | tgccaatgat | 4750 |
| 4751 | gttacagatg | agatggtcag | actaaactgg | ctgacggaat | ttatgcctct | 4800 |
| 4801 | tccgaccatc | aagcatttta | tccgtactcc | tgatgatgca | tggttactca | 4850 |
| 4851 | ccactgcgat | ccccgggaaa | acagcattcc | aggtattaga | agaatatcct | 4900 |
| 4901 | gattcaggtg | aaaatattgt | tgatgcgctg | gcagtgttcc | tgcgccggtt | 4950 |
| 4951 | gcattcgatt | cctgtttgta | attgtccttt | taacagcgat | cgcgtatttc | 5000 |
| 5001 | gtctcgctca | ggcgcaatca | cgaatgaata | acggtttggt | tgatgcgagt | 5050 |
| 5051 | gattttgatg | acgagcgtaa | tggctggcct | gttgaacaag | tctggaaaga | 5100 |
| 5101 | aatgcataaa | cttttgccat | tctcaccgga | ttcagtcgtc | actcatggtg | 5150 |
| 5151 | atttctcact | tgataacctt | atttttgacg | aggggaaatt | aataggttgt | 5200 |
| 5201 | attgatgttg | gacgagtcgg | aatcgcagac | cgataccagg | atcttgccat | 5250 |
| 5251 | cctatggaac | tgcctcggtg | agttttctcc | ttcattacag | aaacggcttt | 5300 |
| 5301 | ttcaaaaata | tggtattgat | aatcctgata | tgaataaatt | gcagtttcat | 5350 |
| 5351 | ttgatgctcg | atgagttttt | ctaagaatta | attcatgagc | ggatacatat | 5400 |
| 5401 | ttgaatgtat | ttagaaaaat | aaacaaatag | gggttccgcg | cacatttccc | 5450 |
| 5451 | cgaaaagtgc | cacctgaaat | tgtaaacgtt | aatattttgt | taaaattcgc | 5500 |
| 5501 | gttaaatttt | tgttaaatca | gctcattttt | taaccaatag | gccgaaatcg | 5550 |
| 5551 | gcaaaatccc | ttataaatca | aaagaataga | ccgagatagg | gttgagtgtt | 5600 |
| 5601 | gttccagttt | ggaacaagag | tccactatta | aagaacgtgg | actccaacgt | 5650 |
| 5651 | caaagggcga | aaaaccgtct | atcagggcga | tggcccacta | cgtgaaccat | 5700 |
| 5701 | caccctaatc | aagttttttg | gggtcgaggt | gccgtaaagc | actaaatcgg | 5750 |
| 5751 | aaccctaaag | ggagcccccg | atttagagct | tgacggggaa | agccggcgaa | 5800 |
| 5801 | cgtggcgaga | aaggaaggga | agaaagcgaa | aggagcgggc | gctagggcgc | 5850 |
| 5851 | tggcaagtgt | agcggtcacg | ctgcgcgtaa | ccaccacacc | cgccgcgctt | 5900 |
| 5901 | aatgcgccgc | tacagggcgc | gtcccattcg | cca | 5933 |
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pET-41a(+)
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