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- 2025年11月21日
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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
pETBlue-2
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号: 60908-2761
质粒名称: pETBlue-2
其他名称:
质粒大小（bp）: 3653
抗性: Ampicillin
质粒类型: Bacterial Expression
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: His (Cterm), HSV (Cterm)
测序引物:
GenBank号:
其他属性:
| Notes: |
Blue/white identification; unique MCS; His and HSV tagged
|
|---|
质粒图谱:
| Feature Name | Start | End | |
|---|---|---|---|
| T7_promoter | 1 | 19 | |
| lacO | 19 | 46 | |
| M13_pUC_fwd_primer | 188 | 210 | |
| T7_transl_en_RBS | 254 | 270 | |
| rrnB_terminator | 619 | 776 | |
| rrnB_T1_terminator | 742 | 785 | |
| rrnB_T2_terminator | 917 | 944 | |
| T7_Terminal_primer | 1016 | 998 | |
| Ampicillin | 2526 | 1666 | |
| pBR322_origin | 2603 | 3222 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 3 | 2526 | 1666 |
| Enzyme Name | Cut |
|---|---|
| XbaI | 47 |
| BglII | 223 |
| NheI | 235 |
| NcoI | 276 |
| EcoRV | 285 |
| SmaI | 290 |
| XmaI | 288 |
| SacI | 297 |
| BamHI | 301 |
| EcoRI | 307 |
| AscI | 320 |
| PstI | 330 |
| AatII | 336 |
| SalI | 335 |
| KpnI | 345 |
| ClaI | 348 |
| BstBI | 359 |
| HindIII | 362 |
| NotI | 369 |
| EagI | 369 |
| PvuII | 379 |
| PmlI | 389 |
| XhoI | 431 |
| PacI | 464 |
| AgeI | 3599 |
质粒序列:
| 1 | taatacgact | cactataggg | gaattgtgag | cggataacaa | ttcccctcta | 50 |
| 51 | gacttacaat | ttccattcgc | cattcaggct | gcgcaactgt | tgggaagggc | 100 |
| 101 | gatcggtacg | ggcctcttcg | ctattacgcc | agcttgcgaa | cggtgggtgc | 150 |
| 151 | gctgcaaggc | gattaagttg | ggtaacgcca | ggattctccc | agtcacgacg | 200 |
| 201 | ttgtaaaacg | acggccagcg | agagatcttg | attggctagc | agaataattt | 250 |
| 251 | tgtttaactt | taagaaggag | atataccatg | gcgatatccc | gggagctcgt | 300 |
| 301 | ggatccgaat | tctgtacagg | cgcgcctgca | ggacgtcgac | ggtaccatcg | 350 |
| 351 | atacgcgttc | gaagcttgcg | gccgcacagc | tgtatacacg | tgcaagccag | 400 |
| 401 | ccagaactcg | ctcctgaaga | cccagaggat | ctcgagcacc | accaccacca | 450 |
| 451 | ccactaatgt | taattaagtt | gggcgttgta | atcatagtca | taatcaatac | 500 |
| 501 | tcctgactgc | gttagcaatt | taactgtgat | aaactaccgc | attaaagcta | 550 |
| 551 | ttcgatgata | agctgtcaaa | catgataatt | cttgaagacg | aaagggccta | 600 |
| 601 | ggctgataaa | acagaatttg | cctggcggca | gtagcgcggt | ggtcccacct | 650 |
| 651 | gaccccatgc | cgaactcaga | agtgaaacgc | cgtagcgccg | atggtagtgt | 700 |
| 701 | ggggtctccc | catgcgagag | tagggaactg | ccaggcatca | aataaaacga | 750 |
| 751 | aaggctcagt | cgaaagactg | ggcctttcgt | tttatctgtt | gtttgtcggt | 800 |
| 801 | gaacgctctc | ctgagtagga | caaatccgcc | gggagcggat | ttgaacgttg | 850 |
| 851 | cgaagcaacg | gcccggaggg | tggcgggcag | gacgcccgcc | ataaactgcc | 900 |
| 901 | aggcatcaaa | ttaagcagaa | ggccatcctg | acggatggcc | tttttgcgtt | 950 |
| 951 | tctacaaact | cttttgttta | tttttctaaa | tacattcaaa | tatgtatccg | 1000 |
| 1001 | ctgagcaata | actagcataa | ccccttgggg | cctctaaacg | ggtcttgagg | 1050 |
| 1051 | ggttttttgc | tgaaaggagg | aactatatcc | ggattggcga | atgggacgcg | 1100 |
| 1101 | ccctgtagcg | gcgcattaag | cgcggcgggt | gtggtggtta | cgcgcagcgt | 1150 |
| 1151 | gaccgctaca | cttgccagcg | ccctagcgcc | cgctcctttc | gctttcttcc | 1200 |
| 1201 | cttcctttct | cgccacgttc | gccggctttc | cccgtcaagc | tctaaatcgg | 1250 |
| 1251 | gggctccctt | tagggttccg | atttagtgct | ttacggcacc | tcgaccccaa | 1300 |
| 1301 | aaaacttgat | tagggtgatg | gttcacgtag | tgggccatcg | ccctgataga | 1350 |
| 1351 | cggtttttcg | ccctttgacg | ttggagtcca | cgttctttaa | tagtggactc | 1400 |
| 1401 | ttgttccaaa | ctggaacaac | actcaaccct | atctcggtct | attcttttga | 1450 |
| 1451 | tttataaggg | attttgccga | tttcggccta | ttggttaaaa | aatgagctga | 1500 |
| 1501 | tttaacaaaa | atttaacgcg | aattttaaca | aaatattaac | gtttacaatt | 1550 |
| 1551 | tctggcggca | cgatggcatg | agattatcaa | aaaggatctt | cacctagatc | 1600 |
| 1601 | cttttaaatt | aaaaatgaag | ttttaaatca | atctaaagta | tatatgagta | 1650 |
| 1651 | aacttggtct | gacagttacc | aatgcttaat | cagtgaggca | cctatctcag | 1700 |
| 1701 | cgatctgtct | atttcgttca | tccatagttg | cctgactccc | cgtcgtgtag | 1750 |
| 1751 | ataactacga | tacgggaggg | cttaccatct | ggccccagtg | ctgcaatgat | 1800 |
| 1801 | accgcgagac | ccacgctcac | cggctccaga | tttatcagca | ataaaccagc | 1850 |
| 1851 | cagccggaag | ggccgagcgc | agaagtggtc | ctgcaacttt | atccgcctcc | 1900 |
| 1901 | atccagtcta | ttaattgttg | ccgggaagct | agagtaagta | gttcgccagt | 1950 |
| 1951 | taatagtttg | cgcaacgttg | ttgccattgc | tacaggcatc | gtggtgtcac | 2000 |
| 2001 | gctcgtcgtt | tggtatggct | tcattcagct | ccggttccca | acgatcaagg | 2050 |
| 2051 | cgagttacat | gatcccccat | gttgtgcaaa | aaagcggtta | gctccttcgg | 2100 |
| 2101 | tcctccgatc | gttgtcagaa | gtaagttggc | cgcagtgtta | tcactcatgg | 2150 |
| 2151 | ttatggcagc | actgcataat | tctcttactg | tcatgccatc | cgtaagatgc | 2200 |
| 2201 | ttttctgtga | ctggtgagta | ctcaaccaag | tcattctgag | aatagtgtat | 2250 |
| 2251 | gcggcgaccg | agttgctctt | gcccggcgtc | aatacgggat | aataccgcgc | 2300 |
| 2301 | cacatagcag | aactttaaaa | gtgctcatca | ttggaaaacg | ttcttcgggg | 2350 |
| 2351 | cgaaaactct | caaggatctt | accgctgttg | agatccagtt | cgatgtaacc | 2400 |
| 2401 | cactcgtgca | cccaactgat | cttcagcatc | ttttactttc | accagcgttt | 2450 |
| 2451 | ctgggtgagc | aaaaacagga | aggcaaaatg | ccgcaaaaaa | gggaataagg | 2500 |
| 2501 | gcgacacgga | aatgttgaat | actcatactc | ttcctttttc | aatcatgacc | 2550 |
| 2551 | aaaatccctt | aacgtgagtt | ttcgttccac | tgagcgtcag | accccgtaga | 2600 |
| 2601 | aaagatcaaa | ggatcttctt | gagatccttt | ttttctgcgc | gtaatctgct | 2650 |
| 2651 | gcttgcaaac | aaaaaaacca | ccgctaccag | cggtggtttg | tttgccggat | 2700 |
| 2701 | caagagctac | caactctttt | tccgaaggta | actggcttca | gcagagcgca | 2750 |
| 2751 | gataccaaat | actgtccttc | tagtgtagcc | gtagttaggc | caccacttca | 2800 |
| 2801 | agaactctgt | agcaccgcct | acatacctcg | ctctgctaat | cctgttacca | 2850 |
| 2851 | gtggctgctg | ccagtggcga | taagtcgtgt | cttaccgggt | tggactcaag | 2900 |
| 2901 | acgatagtta | ccggataagg | cgcagcggtc | gggctgaacg | gggggttcgt | 2950 |
| 2951 | gcacacagcc | cagcttggag | cgaacgacct | acaccgaact | gagataccta | 3000 |
| 3001 | cagcgtgagc | tatgagaaag | cgccacgctt | cccgaaggga | gaaaggcgga | 3050 |
| 3051 | caggtatccg | gtaagcggca | gggtcggaac | aggagagcgc | acgagggagc | 3100 |
| 3101 | ttccaggggg | aaacgcctgg | tatctttata | gtcctgtcgg | gtttcgccac | 3150 |
| 3151 | ctctgacttg | agcgtcgatt | tttgtgatgc | tcgtcagggg | ggcggagcct | 3200 |
| 3201 | atggaaaaac | gccagcaacg | cggccttttt | acggttcctg | gccttttgct | 3250 |
| 3251 | ggccttttgc | tcacatgttc | tttcctgcgt | tatcccctga | ttctgtggat | 3300 |
| 3301 | aaccgtatta | ccgcctttga | gtgagctgat | accgctcgcc | gcagccgaac | 3350 |
| 3351 | gaccgagcgc | agcgagtcag | tgagcgagga | agccggcgat | aatggcctgc | 3400 |
| 3401 | ttctcgccga | aacgtttggt | ggcgggacca | gtgacgaagg | cttgagcgag | 3450 |
| 3451 | ggcgtgcaag | attccgaata | ccgcaagcga | caggccgatc | atcgtcgcgc | 3500 |
| 3501 | tccagcgaaa | gcggtcctcg | ccgaaaatga | cccagagcgc | tgccggcacc | 3550 |
| 3551 | tgtcctacga | gttgcatgat | aaagaagaca | gtcataagtg | cggcgacgac | 3600 |
| 3601 | cggtgaattg | tgagcgctca | caattctcgt | gacatcataa | cgtcccgcga | 3650 |
| 3651 | aat | 3653 |
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pETBlue-2
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