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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
pETBlue-1
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号: 60908-2760
质粒名称: pETBlue-1
其他名称:
质粒大小（bp）: 3476
抗性: Ampicillin
质粒类型: Bacterial Expression
是否病毒: Nonviral
启动子: unspecified
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: unspecified
测序引物:
GenBank号:
其他属性: Blue/white identification; unique MCS.
质粒图谱:
| Feature Name | Start | End | |
|---|---|---|---|
| T7_promoter | 1 | 19 | |
| lacO | 19 | 46 | |
| M13_pUC_fwd_primer | 188 | 210 | |
| T7_transl_en_RBS | 254 | 270 | |
| rrnB_terminator | 442 | 599 | |
| rrnB_T1_terminator | 565 | 608 | |
| rrnB_T2_terminator | 740 | 767 | |
| T7_Terminal_primer | 839 | 821 | |
| Ampicillin | 2349 | 1489 | |
| pBR322_origin | 2426 | 3045 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 3 | 2349 | 1489 |
| Enzyme Name | Cut |
|---|---|
| XbaI | 47 |
| BglII | 223 |
| NheI | 235 |
| EcoRV | 278 |
| EcoRI | 282 |
| XmaI | 291 |
| SmaI | 293 |
| AgeI | 3422 |
质粒序列:
| 1 | taatacgact | cactataggg | gaattgtgag | cggataacaa | ttcccctcta | 50 |
| 51 | gacttacaat | ttccattcgc | cattcaggct | gcgcaactgt | tgggaagggc | 100 |
| 101 | gatcggtacg | ggcctcttcg | ctattacgcc | agcttgcgaa | cggtgggtgc | 150 |
| 151 | gctgcaaggc | gattaagttg | ggtaacgcca | ggattctccc | agtcacgacg | 200 |
| 201 | ttgtaaaacg | acggccagcg | agagatcttg | attggctagc | agaataattt | 250 |
| 251 | tgtttaactt | taagaaggag | atatagatat | cgaattcctg | cccgggcgtt | 300 |
| 301 | gtaatcatag | tcataatcaa | tactcctgac | tgcgttagca | atttaactgt | 350 |
| 351 | gataaactac | cgcattaaag | ctattcgatg | ataagctgtc | aaacatgata | 400 |
| 401 | attcttgaag | acgaaagggc | ctaggctgat | aaaacagaat | ttgcctggcg | 450 |
| 451 | gcagtagcgc | ggtggtccca | cctgacccca | tgccgaactc | agaagtgaaa | 500 |
| 501 | cgccgtagcg | ccgatggtag | tgtggggtct | ccccatgcga | gagtagggaa | 550 |
| 551 | ctgccaggca | tcaaataaaa | cgaaaggctc | agtcgaaaga | ctgggccttt | 600 |
| 601 | cgttttatct | gttgtttgtc | ggtgaacgct | ctcctgagta | ggacaaatcc | 650 |
| 651 | gccgggagcg | gatttgaacg | ttgcgaagca | acggcccgga | gggtggcggg | 700 |
| 701 | caggacgccc | gccataaact | gccaggcatc | aaattaagca | gaaggccatc | 750 |
| 751 | ctgacggatg | gcctttttgc | gtttctacaa | actcttttgt | ttatttttct | 800 |
| 801 | aaatacattc | aaatatgtat | ccgctgagca | ataactagca | taaccccttg | 850 |
| 851 | gggcctctaa | acgggtcttg | aggggttttt | tgctgaaagg | aggaactata | 900 |
| 901 | tccggattgg | cgaatgggac | gcgccctgta | gcggcgcatt | aagcgcggcg | 950 |
| 951 | ggtgtggtgg | ttacgcgcag | cgtgaccgct | acacttgcca | gcgccctagc | 1000 |
| 1001 | gcccgctcct | ttcgctttct | tcccttcctt | tctcgccacg | ttcgccggct | 1050 |
| 1051 | ttccccgtca | agctctaaat | cgggggctcc | ctttagggtt | ccgatttagt | 1100 |
| 1101 | gctttacggc | acctcgaccc | caaaaaactt | gattagggtg | atggttcacg | 1150 |
| 1151 | tagtgggcca | tcgccctgat | agacggtttt | tcgccctttg | acgttggagt | 1200 |
| 1201 | ccacgttctt | taatagtgga | ctcttgttcc | aaactggaac | aacactcaac | 1250 |
| 1251 | cctatctcgg | tctattcttt | tgatttataa | gggattttgc | cgatttcggc | 1300 |
| 1301 | ctattggtta | aaaaatgagc | tgatttaaca | aaaatttaac | gcgaatttta | 1350 |
| 1351 | acaaaatatt | aacgtttaca | atttctggcg | gcacgatggc | atgagattat | 1400 |
| 1401 | caaaaaggat | cttcacctag | atccttttaa | attaaaaatg | aagttttaaa | 1450 |
| 1451 | tcaatctaaa | gtatatatga | gtaaacttgg | tctgacagtt | accaatgctt | 1500 |
| 1501 | aatcagtgag | gcacctatct | cagcgatctg | tctatttcgt | tcatccatag | 1550 |
| 1551 | ttgcctgact | ccccgtcgtg | tagataacta | cgatacggga | gggcttacca | 1600 |
| 1601 | tctggcccca | gtgctgcaat | gataccgcga | gacccacgct | caccggctcc | 1650 |
| 1651 | agatttatca | gcaataaacc | agccagccgg | aagggccgag | cgcagaagtg | 1700 |
| 1701 | gtcctgcaac | tttatccgcc | tccatccagt | ctattaattg | ttgccgggaa | 1750 |
| 1751 | gctagagtaa | gtagttcgcc | agttaatagt | ttgcgcaacg | ttgttgccat | 1800 |
| 1801 | tgctacaggc | atcgtggtgt | cacgctcgtc | gtttggtatg | gcttcattca | 1850 |
| 1851 | gctccggttc | ccaacgatca | aggcgagtta | catgatcccc | catgttgtgc | 1900 |
| 1901 | aaaaaagcgg | ttagctcctt | cggtcctccg | atcgttgtca | gaagtaagtt | 1950 |
| 1951 | ggccgcagtg | ttatcactca | tggttatggc | agcactgcat | aattctctta | 2000 |
| 2001 | ctgtcatgcc | atccgtaaga | tgcttttctg | tgactggtga | gtactcaacc | 2050 |
| 2051 | aagtcattct | gagaatagtg | tatgcggcga | ccgagttgct | cttgcccggc | 2100 |
| 2101 | gtcaatacgg | gataataccg | cgccacatag | cagaacttta | aaagtgctca | 2150 |
| 2151 | tcattggaaa | acgttcttcg | gggcgaaaac | tctcaaggat | cttaccgctg | 2200 |
| 2201 | ttgagatcca | gttcgatgta | acccactcgt | gcacccaact | gatcttcagc | 2250 |
| 2251 | atcttttact | ttcaccagcg | tttctgggtg | agcaaaaaca | ggaaggcaaa | 2300 |
| 2301 | atgccgcaaa | aaagggaata | agggcgacac | ggaaatgttg | aatactcata | 2350 |
| 2351 | ctcttccttt | ttcaatcatg | accaaaatcc | cttaacgtga | gttttcgttc | 2400 |
| 2401 | cactgagcgt | cagaccccgt | agaaaagatc | aaaggatctt | cttgagatcc | 2450 |
| 2451 | tttttttctg | cgcgtaatct | gctgcttgca | aacaaaaaaa | ccaccgctac | 2500 |
| 2501 | cagcggtggt | ttgtttgccg | gatcaagagc | taccaactct | ttttccgaag | 2550 |
| 2551 | gtaactggct | tcagcagagc | gcagatacca | aatactgtcc | ttctagtgta | 2600 |
| 2601 | gccgtagtta | ggccaccact | tcaagaactc | tgtagcaccg | cctacatacc | 2650 |
| 2651 | tcgctctgct | aatcctgtta | ccagtggctg | ctgccagtgg | cgataagtcg | 2700 |
| 2701 | tgtcttaccg | ggttggactc | aagacgatag | ttaccggata | aggcgcagcg | 2750 |
| 2751 | gtcgggctga | acggggggtt | cgtgcacaca | gcccagcttg | gagcgaacga | 2800 |
| 2801 | cctacaccga | actgagatac | ctacagcgtg | agctatgaga | aagcgccacg | 2850 |
| 2851 | cttcccgaag | ggagaaaggc | ggacaggtat | ccggtaagcg | gcagggtcgg | 2900 |
| 2901 | aacaggagag | cgcacgaggg | agcttccagg | gggaaacgcc | tggtatcttt | 2950 |
| 2951 | atagtcctgt | cgggtttcgc | cacctctgac | ttgagcgtcg | atttttgtga | 3000 |
| 3001 | tgctcgtcag | gggggcggag | cctatggaaa | aacgccagca | acgcggcctt | 3050 |
| 3051 | tttacggttc | ctggcctttt | gctggccttt | tgctcacatg | ttctttcctg | 3100 |
| 3101 | cgttatcccc | tgattctgtg | gataaccgta | ttaccgcctt | tgagtgagct | 3150 |
| 3151 | gataccgctc | gccgcagccg | aacgaccgag | cgcagcgagt | cagtgagcga | 3200 |
| 3201 | ggaagccggc | gataatggcc | tgcttctcgc | cgaaacgttt | ggtggcggga | 3250 |
| 3251 | ccagtgacga | aggcttgagc | gagggcgtgc | aagattccga | ataccgcaag | 3300 |
| 3301 | cgacaggccg | atcatcgtcg | cgctccagcg | aaagcggtcc | tcgccgaaaa | 3350 |
| 3351 | tgacccagag | cgctgccggc | acctgtccta | cgagttgcat | gataaagaag | 3400 |
| 3401 | acagtcataa | gtgcggcgac | gaccggtgaa | ttgtgagcgc | tcacaattct | 3450 |
| 3451 | cgtgacatca | taacgtcccg | cgaaat | 3476 |
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pETBlue-1
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