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- 详细信息
- 技术资料
- 库存:
大量
- 英文名:
pGCAT-C
- 保质期:
1年
- 供应商:
再康生物
- 保存条件:
低温运输,-20℃保存
质粒编号:
质粒名称: pGCAT-C
其他名称:
质粒大小(bp): 4552
抗性:
质粒类型: Unspecified
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性: Restriction digests of the clone give the following sizes (kb): PvuII–3.0, 1.5; HincII–3.0, 1.5; EcoRI–4.25, 0.3. (ATCC staff) The polylinker is in opposite orientation in pGCAT-A (ATCC 37640) and pGCAT-C (ATCC 37641). The sequence at the junction of the multiple cloning site and CAT is GAATTCGAGC and at the junction of SV40 and SP6 is GGATCAGCTT. (personal communication) BamHI/HindIII fragment of pSB1 (CAT) was cloned into the HindIII site of pGEM-4. 2 hrs after ligation began the remaining BamHI and HindIII sites were filled in. Contains SV40 polyadenylation site and donor and acceptor sites of SV40 small-t intron. A plasmid shuttle vector for investigating promoter and/or enhancer elements. Has a polylinker upstream of reporter CAT gene, SP6 and T7 promoters for in vitro RNA synthesis and low basal CAT expression in eukaryotic cells. Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, vertebrate cells, E.coli.
质粒图谱:
质粒序列:
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