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- 详细信息
- 技术资料
- 库存：
1
- 英文名：
EpiQuik Global Histone H3-K9 Methylation Assay Kit
- 保质期：
6个月
- 供应商：
上海再康
- 保存条件：
4℃保存
- 通用组蛋白H3-K9甲基化分析试剂盒详细信息：
-
Product Overview
The EpiQuik™ Global Histone H3-K9 Methylation Assay Kit is suitable for specifically measuring global histone H3-K9 methylation using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
WHY CHOOSE THE EPIQUIK™ GLOBAL HISTONE H3-K9 METHYLATION ASSAY KIT?
- Quick and efficient, which can be finished within 5 hours.
- Innovative colorimetric assay with no need for radioactivity, electrophoresis, and chromatography.
- Strip microwell format makes the assay flexible: manual or high throughput.
- Simple, reliable, and consistent assay conditions.
通用组蛋白H3-K9甲基化分析试剂盒Principle & Procedure
The EpiQuik™ Global H3-K9 Methylation Assay Kit is designed for measuring global histone H3-K9 methylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The methylated histone H3-K9 can be recognized with high-affinity antibody. The ratio or amount of methylated H3-K9 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.
SCHEMATIC PROCEDURE: 通用组蛋白H3-K9甲基化分析试剂盒Product Components
GC1 (10x lysis buffer)
GC2 (Extraction buffer)
GC3 (10x wash buffer)
GC4 (Histone buffer)
GC5 (Blocking buffer)
GC6 (Antibody buffer)
GC7 (capture antibody, 100 µg/ml)*
GC8 (detection antibody, 400 µg/ml)*
GC9 (developing solution)
GC10 (stop solution)
Methylated H3-K9 control (60 µg/ml)*
8 well assay strips (with frame)
User guide
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* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.通用组蛋白H3-K9甲基化分析试剂盒User Guide & MSDS
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通用组蛋白H3-K9甲基化分析试剂盒
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