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- 详细信息
- 技术资料
- 库存:
大量
- 英文名:
lambdaSK22
- 保质期:
1年
- 供应商:
再康生物
- 保存条件:
低温运输,-20℃保存
质粒编号:
质粒名称: lambdaSK22
其他名称:
质粒大小(bp): 47800
抗性:
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性: Restriction digests of the clone give the following sizes (kb): BamHI–25.0, 13.5, 9.5; EcoRI–25.0, 13.5, 9.5; SalI–20.0, 9.5, 9.2, 4.2, 3.9. (ATCC staff) Vector permitting positive selection (Spi) of recombinants. Useful for generating jumping and linking libraries and conversion to phagemid (without selection). Permits construction using partial filling-in. The sizes of the major components are (kb): arms–20.3, 9.2; stuffer fragment–13.7; phagemid segments–3.9 (longer arm), 0.7. The polylinker sites given are those associated with the longer arm. The sites associated with the other arm are: SalI BamHI EcoRI XhoI NotI NaeI SfiI AvrII NheI NcoI XbaI. The restriction sites to be used for conversion to phagemid are: ClaI BspMII SalI. The order of the major features in this plasmid is: arm – ClaI BspMII SalI – pMB1 ori – ampR – M13 ori – SacI/MCS/SalI – stuffer – SalI/MCS/XbaI – ClaI SalI – arm. [2] Deposited by: Zabarovsky E.R., Winberg G.R., Klein G.R. Hosts: E.coli.
质粒图谱:
质粒序列:
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