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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 英文名：
Recombinant Lysophosphatidylcholine Acyltransferase 1 (LPCAT1)
- 保质期：
12个月
- 供应商：
上海再康
- 保存条件：
-20℃
溶血卵磷脂酰基转移酶1(LPCAT1)重组蛋白
Recombinant Lysophosphatidylcholine Acyltransferase 1 (LPCAT1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | zk529Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 90% |
| UOM | 50ug |
| Predicted Molecular Mass | 48.7kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
溶血卵磷脂酰基转移酶1(LPCAT1)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The sequence of the target protein is listed below.
P LALVASLGSA EKEPEQPPAL WRKVVDFLLK AIMRTMWFAG GFHRVAVKGR QALPTEAAIL TLAPHSSYFD AIPVTMTMSS IVMKAESRDI PIWGTLIQYI RPVFVSRSDQ DSRRKTVEEI KRRAQSNGKW PQIMIFPEGT CTNRTCLITF KPGAFIPGAP VQPVVLR
P LALVASLGSA EKEPEQPPAL WRKVVDFLLK AIMRTMWFAG GFHRVAVKGR QALPTEAAIL TLAPHSSYFD AIPVTMTMSS IVMKAESRDI PIWGTLIQYI RPVFVSRSDQ DSRRKTVEEI KRRAQSNGKW PQIMIFPEGT CTNRTCLITF KPGAFIPGAP VQPVVLR
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
溶血卵磷脂酰基转移酶1(LPCAT1)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
溶血卵磷脂酰基转移酶1(LPCAT1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
溶血卵磷脂酰基转移酶1(LPCAT1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
的表达系统会造成质粒的不稳定，细胞生长速度的下降和重组蛋白产量的降低[32，33]。Lanzer和Bujard曾对常用的lac启动子-操纵子系统进行了广泛的研究，证明操纵子放在启动子序列的不同位置会造成70倍差异的抑制。将17bp的操纵子置于-10和-35六聚体区之间所形成的抑制比将其放在-35区的上游或-10区的下游要高50-70倍[34]。 启动子的第三个特性是其简便和廉价的可诱导性。大量生产蛋白质最常用的启动子是热诱导（λPL）和化学诱导(trp)启动子。异丙基硫代-β-D-半乳糖
和洗脱条件，为重组蛋白的生产、检测和纯化提供了一个有力的工具。 使用基因融合表达系统在E.coli中表达外源基因已经越来越受欢迎。这在很大程度上归因于融合系统能够产生大量的可溶性的融合蛋白。谷胱甘肽S-转移酶(GST)、麦芽糖结合蛋白(MBP)以及硫氧还蛋白(Trx)均已经被证实能非常成功地生产正确折叠、有生物活性的蛋白质，能明显提高在E.coli细胞质中产生的融合蛋白的可溶性，并能抑制包涵体的形成[159，194]。其中每一种都备有方便的纯化方法，可将融合蛋白与细胞污染物分开。已经建立
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溶血卵磷脂酰基转移酶1(LPCAT1)重组蛋白
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