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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
pFL34
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号:
质粒名称: pFL34
其他名称:
质粒大小（bp）: 3799
抗性: Ampicillin
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号: X70479
其他属性: pFL34 was constructed from pUC19 plasmid where two alu I sites were modified. The site 629 was replaced by a BglII linker and the site 747 by a ClaI site. The yeast selectable marker has been cloned in the BglII site. The order of the major features in this plasmid is:EcoRI/MCS/HindIII – URA3 – pMB1 ori – ampR. YI-type shuttle integration vector permitting visual detection of recombinants based on alpha complementation. Restriction digests of the clone give the following sizes (kb): BamHI–3.9; BglII–2.8, 1.1; ClaI–3.9. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 311965 Hosts: E.coli HB101, S.cerevisiae, E.coli, Saccharomyces. Related vectors: pUC19, yeast 2-micron plasmid.
质粒图谱:
质粒序列:
| 1 | tcgcgcgttt | cggtgatgac | ggtgaaaacc | tctgacacat | gcagctcccg | 50 |
| 51 | gagacggtca | cagcttgtct | gtaagcggat | gccgggagca | gacaagcccg | 100 |
| 101 | tcagggcgcg | tcagcgggtg | ttggcgggtg | tcggggctgg | cttaactatg | 150 |
| 151 | cggcatcaga | gcagattgta | ctgagagtgc | accatatgcg | gtgtgaaata | 200 |
| 201 | ccgcacagat | gcgtaaggag | aaaataccgc | atcaggcgcc | attcgccatt | 250 |
| 251 | caggctgcgc | aactgttggg | aagggcgatc | ggtgcgggcc | tcttcgctat | 300 |
| 301 | tacgccagct | ggcgaaaggg | ggatgtgctg | caaggcgatt | aagttgggta | 350 |
| 351 | acgccagggt | tttcccagtc | acgacgttgt | aaaacgacgg | ccagtgaatt | 400 |
| 401 | cgagctcggt | acccggggat | cctctagagt | cgacctgcag | gcatgcaagc | 450 |
| 451 | ttggcgtaat | catggtcata | gctgtttcct | gtgtgaaatt | gttatccgct | 500 |
| 501 | cacaattcca | cacaacatac | gagccggaag | cataaagtgt | aaagcctggg | 550 |
| 551 | gtgcctaatg | agtgagctaa | ctcacattaa | ttgcgttgcg | ctcactgccc | 600 |
| 601 | gctttccagt | cgggaaacct | gtcgtgccag | cagatctgag | ctttttcttt | 650 |
| 651 | ccaatttttt | ttttttgtga | gtttagtata | catgcattta | cttataatac | 700 |
| 701 | agttttttag | ttttgctggc | cgcatcttct | caaatatgct | tcccagcctg | 750 |
| 751 | cttttctgta | acgttcaccc | tctaccttag | catcccttcc | ctttgcaaat | 800 |
| 801 | agtcctcttc | caacaataat | aatgtcagat | cctgtagaga | ccacatcatc | 850 |
| 851 | cacggttcta | tactgttgac | ccaatgcgtc | tcccttgtca | tctaaaccca | 900 |
| 901 | caccgggtgt | cataatcaac | caatcgtaac | cttcatctct | tccacccatg | 950 |
| 951 | tctctttgag | caataaagcc | gataacaaaa | tctttgtcgc | tcttcgcaat | 1000 |
| 1001 | gtcaacagta | cccttagtat | attctccagt | agatagggag | cccttgcatg | 1050 |
| 1051 | acaattctgc | taacatcaaa | aggcctctag | gttcctttgt | tacttcttct | 1100 |
| 1101 | gccgcctgct | tcaaaccgct | aacaatacct | gggcccacca | caccgtgtgc | 1150 |
| 1151 | attcgtaatg | tctgcccatt | ctgctattct | gtatacaccc | gcagagtact | 1200 |
| 1201 | gcaatttgac | tgtattacca | atgtcagcaa | attttctgtc | ttcgaagagt | 1250 |
| 1251 | aaaaaattgt | acttggcgga | taatgccttt | agcggcttaa | ctgtgccctc | 1300 |
| 1301 | catggaaaaa | tcagtcaaga | tatccacatg | tgtttttagt | aaacaaattt | 1350 |
| 1351 | tgggacctaa | tgcttcaact | aactccagta | attccttggt | ggtacgaaca | 1400 |
| 1401 | tccaatgaag | cacacaagtt | tgtttgcttt | tcgtgcatga | tattaaatag | 1450 |
| 1451 | cttggcagca | acaggactag | gatgagtagc | agcacgttcc | ttatatgtag | 1500 |
| 1501 | ctttcgacat | gatttatctt | cgtttccggt | ttttgttctg | tgcagttggg | 1550 |
| 1551 | ttaagaatac | tgggcaattt | catgtttctt | caacactaca | tatgcgtata | 1600 |
| 1601 | tataccaatc | taagtctgtg | ctccttcctt | cgttcttcct | tctgttcgga | 1650 |
| 1651 | gattaccgaa | tcaaaaaaat | ttcaaagaaa | ccgaaatcaa | aaaaaagaat | 1700 |
| 1701 | aaaaaaaaaa | tgatgaattg | aaaagccaga | tctgctgcat | taatgaatcg | 1750 |
| 1751 | gccaacgcgc | ggggagaggc | ggtttgcgta | ttgggcgctc | ttccgcttcc | 1800 |
| 1801 | tcgctcactg | actcgctgcg | ctcggtcgtt | cggctgcggc | gagcggtatc | 1850 |
| 1851 | agcatcgatg | ctcactcaaa | ggtcggtaat | acggttatcc | acagaatcag | 1900 |
| 1901 | gggataacgc | aggaaagaac | atgtgagcaa | aaggccagca | aaaggccagg | 1950 |
| 1951 | aaccgtaaaa | aggccgcgtt | gctggcgttt | ttccataggc | tccgcccccc | 2000 |
| 2001 | tgacgagcat | cacaaaaatc | gacgctcaag | tcagaggtgg | cgaaacccga | 2050 |
| 2051 | caggactata | aagataccag | gcgtttcccc | ctggaagctc | cctcgtgcgc | 2100 |
| 2101 | tctcctgttc | cgaccctgcc | gcttaccgga | tacctgtccg | cctttctccc | 2150 |
| 2151 | ttcgggaagc | gtggcgcttt | ctcatagctc | acgctgtagg | tatctcagtt | 2200 |
| 2201 | cggtgtaggt | cgttcgctcc | aagctgggct | gtgtgcacga | accccccgtt | 2250 |
| 2251 | cagcccgacc | gctgcgcctt | atccggtaac | tatcgtcttg | agtccaaccc | 2300 |
| 2301 | ggtaagacac | gacttatcgc | cactggcagc | agccactggt | aacaggatta | 2350 |
| 2351 | gcagagcgag | gtatgtaggc | ggtgctacag | agttcttgaa | gtggtggcct | 2400 |
| 2401 | aactacggct | acactagaag | gacagtattt | ggtatctgcg | ctctgctgaa | 2450 |
| 2451 | gccagttacc | ttcggaaaaa | gagttggtag | ctcttgatcc | ggcaaacaaa | 2500 |
| 2501 | ccaccgctgg | tagcggtggt | ttttttgttt | gcaagcagca | gattacgcgc | 2550 |
| 2551 | agaaaaaaag | gatctcaaga | agatcctttg | atcttttcta | cggggtctga | 2600 |
| 2601 | cgctcagtgg | aacgaaaact | cacgttaagg | gattttggtc | atgagattat | 2650 |
| 2651 | caaaaaggat | cttcacctag | atccttttaa | attaaaaatg | aagttttaaa | 2700 |
| 2701 | tcaatctaaa | gtatatatga | gtaaacttgg | tctgacagtt | accaatgctt | 2750 |
| 2751 | aatcagtgag | gcacctatct | cagcgatctg | tctatttcgt | tcatccatag | 2800 |
| 2801 | ttgcctgact | ccccgtcgtg | tagataacta | cgatacggga | gggcttacca | 2850 |
| 2851 | tctggcccca | gtgctgcaat | gataccgcga | gacccacgct | caccggctcc | 2900 |
| 2901 | agatttatca | gcaataaacc | agccagccgg | aagggccgag | cgcagaagtg | 2950 |
| 2951 | gtcctgcaac | tttatccgcc | tccatccagt | ctattaattg | ttgccgggaa | 3000 |
| 3001 | gctagagtaa | gtagttcgcc | agttaatagt | ttgcgcaacg | ttgttgccat | 3050 |
| 3051 | tgctacaggc | atcgtggtgt | cacgctcgtc | gtttggtatg | gcttcattca | 3100 |
| 3101 | gctccggttc | ccaacgatca | aggcgagtta | catgatcccc | catgttgtgc | 3150 |
| 3151 | aaaaaagcgg | ttagctcctt | cggtcctccg | atcgttgtca | gaagtaagtt | 3200 |
| 3201 | ggccgcagtg | ttatcactca | tggttatggc | agcactgcat | aattctctta | 3250 |
| 3251 | ctgtcatgcc | atccgtaaga | tgcttttctg | tgactggtga | gtactcaacc | 3300 |
| 3301 | aagtcattct | gagaatagtg | tatgcggcga | ccgagttgct | cttgcccggc | 3350 |
| 3351 | gtcaatacgg | gataataccg | cgccacatag | cagaacttta | aaagtgctca | 3400 |
| 3401 | tcattggaaa | acgttcttcg | gggcgaaaac | tctcaaggat | cttaccgctg | 3450 |
| 3451 | ttgagatcca | gttcgatgta | acccactcgt | gcacccaact | gatcttcagc | 3500 |
| 3501 | atcttttact | ttcaccagcg | tttctgggtg | agcaaaaaca | ggaaggcaaa | 3550 |
| 3551 | atgccgcaaa | aaagggaata | agggcgacac | ggaaatgttg | aatactcata | 3600 |
| 3601 | ctcttccttt | ttcaatatta | ttgaagcatt | tatcagggtt | attgtctcat | 3650 |
| 3651 | gagcggatac | atatttgaat | gtatttagaa | aaataaacaa | ataggggttc | 3700 |
| 3701 | cgcgcacatt | tccccgaaaa | gtgccacctg | acgtctaaga | aaccattatt | 3750 |
| 3751 | atcatgacat | taacctataa | aaataggcgt | atcacgaggc | cctttcgtc | 3799 |
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