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- 详细信息
- 文献和实验
- 技术资料
- 库存:
1000
- 英文名:
Recombinant Claudin 11 (CLDN11)
- 保质期:
12个月
- 供应商:
上海再康
- 保存条件:
-20℃
| Organism species | Homo sapiens (Human) |
| Product No. | zk299Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | n/a |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验293,CHO-S,DG44和CHO-K1细胞,来源于经筛选转染效率更高的亚细胞系。这些细胞也可用于无血清和限定化学成分培养基。重组蛋白的大规模生产一般在稳定转染的悬浮细胞中进行。这些细胞易于生长到高密度并合成更多蛋白,使用基质珠做为固相支持可以使贴壁细胞悬浮生长。 11转染效率的验证 最有效的验证方式必须是qPCR验证。 荧光观察,使用病毒转染,明白自己病毒的情况,是荧光标记(一般是GFP)还是非荧光标记。质粒转染,可以在载体上添加荧光标记,帮助自己通过荧光观察转染效率。但是荧光并不能完全证明转染
Phenotyping the Claudin 11 Deficiency in Testis: From Histology to Immunohistochemistry
of the BTB in the absence of the claudin 11 protein causes azoospermia and leads to infertility. Specifically, cldn11 deficiency results in sloughing of the cells of the seminiferous epithelium into the lumen. Understanding this pathophysiology has involved
编号:200-13)的说明书中您会看到:Slow to dissolve (缓慢溶解),在重组人IL-11(产品编号:200-11)的说明中会看到: This solution is slow to dissolve.(该溶液溶解缓慢)。这种情况下,您最好能将要溶解的细胞因子或重组蛋白放在水平摇床(shaker)上低速摇一段时间,促使细胞因子或重组蛋白完全溶解。 有些蛋白,如重组人IL-13 Variant (产品编号:200-13A)虽然难溶,但却不需用摇床来加速溶解。其说明书上的备注
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