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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1
- 英文名：
PP2A Alpha Subunit (6G3) Monoclonal Antibody
- 保质期：
6个月
- 供应商：
上海再康
- 保存条件：
4℃保存
- PP2A Alpha Subunit (6G3) Monoclonal Antibody保存于运输说明：
- Store at -20°C
- 详细信息：
-
Description Monoclonal Antibody against PP2A Alpha Subunit, clone 6G3 Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Clone 6G3 Form Ascites Host PP2A Alpha Subunit (6G3) Monoclonal Antibody Rat Species Reactivity Human, bovine, mouse, frog IsoType IgG2a [Ab] 1-2 mg/mL Specificity Protein phosphatase 2A (PP2A) is the most abundant phosphatase specific for serine- and threonine- phosphorylation in mammalian cells. Dephosphorylation of substrates targeted by PP2A plays a vital role in a large variety of cellular processes including signal transduction, regulation of the cell cycle, gene expression and development. This monoclonal antibody was raised against human Aα subunit 7. Clone 6G3 recognizes an epitope in repeat 15 at the C terminus of the protein and immunoprecipites only the free A subunit. Uses This antibody is effective in immunoblotting and immunoprecipitation (IP). Suggested Working Dilution PP2A Alpha Subunit (6G3) Monoclonal Antibody The optimal working dilution should be determined for each specific assay condition.. - Western blot: 1:1,000
- IP: 1:150
Storage Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. References Kremmer E, Ohst K, Kiefer J, Brewis N, Walter G. Separation of PP2A core enzyme and holoenzyme with monoclonal antibodies against the regulatory A subunit: abundant expression of both forms in cells. Mol Cell Biol 17(3):1692-1701, 1997. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - PP2A Alpha Subunit (6G3) Monoclonal Antibody
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文献和实验Molecular Cloning of Antiground-State Proteolytic Antibody Fragments
of this phenomenon (4 ,5 ), it has become clear that hypervariability in antibody genes may permit a natural evolution of enzyme-like sites in antibodies. Additional evidence for this hypothesis consists of observations that monoclonal antibodies (MAb) to a natural
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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