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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1
- 英文名：
RNA Polymerase II H14 Monoclonal Antibody
- 保质期：
6个月
- 供应商：
上海再康
- 保存条件：
4℃保存
- RNA Polymerase II H14 Monoclonal Antibody保存于运输说明：
- Store at -20°C
- 详细信息：
-
Description Monoclonal Antibody against RNA Polymerase II Clone H14 Form Ascites Host Mouse Species Reactivity Extensive (Yeast to Human) IsoType RNA Polymerase II H14 Monoclonal Antibody IgM [Ab] 3-5 mg/mL Specificity The antibody H14 was developed against a purified phosphorylated form of RNA polymerase II extracted from a transformed cell line. Pol II has two physiologically important phosphorylation sites; ser-2 and ser-5. Both sites are found in the heptapeptide repeat YSPTSPS at the C-terminal domain. This antibody recognizes the phosphoserine 5 version of pol II. Uses This antibody is effective in immunoblotting, immunofluorescence (IF), immunoprecipitation (IP), pol II purification and functional studies. Suggested Working Dilution Quality control tests were done by Western and Dot blots. The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. - Western blot: 1:500*
- IF: 1:50-100
- IP: 1:100
Notes RNA Polymerase II H14 Monoclonal Antibody Predicted MW = 220-240 kD
*use casein or BSA in the blocking solution rather than non-fat-dry milkStorage Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. References Ahn SH, Kim M, Buratowski S. Phosphorylation of Serine 2 with the RNA Polymerase II C-Terminal Domain Couples Transcription and 3’ End Processing. Mol. Cell 13:67-76, 2004.
Espinosa JM, Verdun RE, Emerson BM. p53 functions through stress- and promoter-specific recruitment of transcription initiation components before and after DNA damage. Mol. Cell. 12(4):1015-27, 2003.
Pellizzoni L, Charroux B, Rappsilber J, Mann M, Dreyfuss G. A functional interaction between the survival motor neuron complex and RNA polymerase II. J. Cell. Biol. 152(1):75-85, 2001.
Patturajan M, Schulte RJ, Sefton BM, Berezney R, Vincent M, Bensaude O, Warren SL, Corden JL. Growth-related changes in phosphorylation of yeast RNA polymerase II. J. Biol. Chem. 273(8):4689-4694, 1998.
Bregman DB, Du L, van der Zee S, Warren SL. Transcription-dependent redistribution of the large subunit of RNA polymerase II to discrete nuclear domains. J. Cell. Biol. 129:287-298, 1995.Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. RNA Polymerase II H14 Monoclonal Antibody - zk-12532R ARVCF精神分裂症与犰狳重复基因抗体
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RNA Polymerase II H14 Monoclonal Antibody
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