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文献和实验Carboxypeptidase Y (EC 3.4.16.1)
(no suitable chemical method exists for the sequential removal of C-terminal amino acids from a polypeptide). The protein or peptide being analyzed is digested with carboxypeptidase and aliquots removed at timed intervals, and analyzed for the presence of free
are first cleaved with trypsin in water, and subsequently the peptide products are dried and labeled by incubation with trypsin in 18 O-enriched water. One important aspect of this two-step procedure is that peptides, and not proteins, are dried
,with the development of ESI-MS and MALDI-MS.Peptide mass fingerprinting uses the peptide masses obtained by digestion to search the protein and DNA databases,to find proteins that show a similar theoretical digest pattern (see eg .,Shevchenko et al.1996a and b
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