- ￥220
- 再康生物
- ZK-0004889
- 中国
- 2025年11月20日
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- 库存：
大量
- 保修期：
一个月
- 现货状态：
现货
- 供应商：
上海再康生物科技有限公司
- 规格：
13.25cm×15cm/张/5张（13.25×15cm）
中文名:PVDF转移膜 (0.22um)
英文名:PVDF转移膜 0.22u
CAS:Millipore
级别:N/A
分子量:N/A
分子式:N/A
纯度:N/A
储存条件:RT
产品简介Overview
- Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.
Immobilon-P Transfer Membrane:
- 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
- Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity
Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa
Blotting Sandwiches:
- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.
Blotting Filter Paper:
- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.
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文献和实验Protein detection onto PVDF membranes
2-D PAGE and electroblotting onto PVDF membranes have become widely used techniques for the characterization of proteins. Recent improvements have allowed a higher protein load, a better transfer yield and a higher sensitivity in automated
透明板在操作的时候比PVDF板稍微容易点，PVDF板在使用时要进行预处理板，操作时还要小心不要把那层膜捅破了。 透明板就是显示的斑点都是黑色的，不能进行几种因子的同时测定，而PVDF板就可以用斑点颜色的变化一次来测定几种细胞因子。 总结：都有优缺点，个人认为，测一种细胞因子的话还是透明板比较好。
Western杂交时，为确认蛋白是否转至PVDF膜上，可用下列方法对膜上蛋白进行可逆性染色。 1. 氨基黑染色 染液：0.5% Amido Black (w/v), 25% isopropanol (v/v) and 10% acetic acid. 染色：将PVDF膜置于染液中染色数钟，ddH2O 脱色。 2. 考马氏亮蓝染色 染液：0.1% Coomassie Brilliant Blue R-250 (w/v) and 50% methanol (v/v
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