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- 详细信息
- 文献和实验
- 技术资料
- 库存:
1000
- 英文名:
Recombinant Dipeptidyl Peptidase 10 (DPP10)
- 保质期:
12个月
- 供应商:
上海再康
- 保存条件:
-20℃
| Organism species | Homo sapiens (Human) |
| Product No. | zk443Hu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | n/a |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
二肽基肽酶10(DPP10)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验因子的Xa系列。 TAGZyme系统的主要组成部分是一种外切蛋白酶――二肽氨基肽酶Ⅰ(dipeptidyl aminopeptidase I,DAPase),如果你表达的外源蛋白不含有外切酶识别的停顿位点,那么你还需要联合使用谷氨酸循环转移酶(glutamine cyclotransferase,Qcyclase)和焦谷氨酸氨基肽酶(pyroglutamyl aminopeptidase,pGAPase)。这些酶都经过重组,可以通过Ni-NTA填料除去。应用TAGZyme系统的反应是十分灵敏的,不会
值是多少? 缓冲液的PH值随温度变化而变化。下表列出了50mM Tris-HC 溶液在4℃,25℃,37℃时,不同的PH值。 10. 昆虫细胞培养的最适PH值和渗透压是多少? 生长培养基的PH值对细胞的增值和病毒或重组蛋白的生产均会产生影响。对于大部分鳞翅类昆虫细胞系,在PH值 6.0-6.4范围的大部分应用效果良好。培养鳞翅类昆虫细胞系时,培养基的最适渗透压是 345-380mOsm/kg.。为保证可靠和持久的细胞培养方式,减少技术问题,保持PH值和渗透
值会改变吗?对于普通使用的缓冲液,PH值随温度变化而变化。例如 20℃下配制PH7.4 Tris缓冲液,40℃时PH值为 7.4-(2x0.310)=6.789. 室温下(25℃)配制的Tris-HCl溶液,在37℃使用时PH值是多少?缓冲液的PH值随温度变化而变化。下表列出了50mM Tris-HC 溶液在4℃,25℃,37℃时,不同的PH值。10. 昆虫细胞培养的最适PH值和渗透压是多少?生长培养基的PH值对细胞的增值和病毒或重组蛋白的生产均会产生影响。对于大部分鳞翅类昆虫细胞系,在PH
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