- ￥2000 - 8000
- 湖北省武汉市
- Kluyveromyces lactis AME1 重组蛋白表达
- 2025年11月18日
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- 详细信息
- 文献和实验
- 技术资料
- 服务名称：
大肠杆菌系统重组蛋白表达
- 提供商：
普健生物（武汉）科技有限公司
Uniprot号：Q6CUE5
基因名：AME1
蛋白名：Inner kinetochore subunit AME1
蛋白别名：
CENP-U homolog, Constitutive centromere-associated network protein AME1
服务内容：
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 |
基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示：
Q6CUE5相关研究文献：
1. Genome evolution in yeasts.
2. Molecular basis for inner kinetochore configuration through RWD domain-peptide interactions.
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文献和实验Dealing with different methods for Kluyveromyces lactis -galactosidase purification
Several micro-scale chromatography-based procedures for purification of the β-galactosidase from the yeast Kluyveromyces lactis were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific
Nystatin-Rhodamine B Assay for Estimating Activity of Killer Toxin from Kluyveromyces lactis
cells, which stain with the fluorescent dye Rhodamine B. Nevertheless, this test cannot be used to assess the effect of Kluyveromyces lactis killer toxin, since it does not render sensitive cells permeable to this stain but merely causes a Gl block
The budding yeast Kluyveromyces lactis has diverged from the Saccharomyces lineage before the whole-genome duplication and its genome sequence reveals lower redundancy of many genes. Moreover, it shows lower preference for fermentative carbon
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