重组人 Glypican 3/GPC3 蛋白 (Site-Specific PE Conjugation, His Tag)

重组人 Glypican 3/GPC3 蛋白 (Site-Specific PE Conjugation, His Tag)（产品说明）
蛋白名称：Human Glypican 3/GPC3 Protein (Site-Specific PE Conjugation, His Tag)
蛋白构建：A DNA sequence encoding the human GPC3 (P51654-1) (Met 1-His 559) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with PE (Excitation= 495 - 566 nm, Emission Max.= 575 nm).
表达宿主：HEK293 Cells
蛋白活性：1.Flow cytometric analysis of anti-GPC3 CAR expression. 5e5 of anti-GPC3 CAR-293 cells were stained with 100 μL of 1:100 (1 μL stock solution in 100 μL FACS buffer) of PE-conjugated human GPC3 Protein (Site-Specific PE Conjugation, His Tag, Cat. No. 10088-H86H-SP) and negative control protein. Non-transduced 293 cells were used as a control (left). PE signal was used to evaluate the binding activity (QC tested). 2.Binding activity of PE-conjugated GPC3 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and PE-conjugated human GPC3 Protein (Site-Specific PE Conjugation, His Tag, Cat. No. 10088-H86H-SP) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. PE signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Gln 25 & Ser 359
蛋白分子量：The recombinant human GPC3 comprises 546 amino acids and has a predicted molecular mass of 64 kDa, Cleaved partially by a furin-like convertase to N and C-terminal fragment with predicted molecular mass of 38 KDa and 26 kDa.
蛋白NP号：P51654-1
蛋白氨基酸序列：Met 1-His 559