重组人c-Kit蛋白 (Site-Specific PE C

重组人c-Kit蛋白（产品说明）
蛋白名称：Human c-Kit Protein (Site-Specific PE Conjugation, ECD, His Tag)
蛋白构建：A DNA sequence encoding the human Kit (P10721-2) (Met 1-Thr 516) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with PE (Excitation = 495-566 nm, Emission Max.= 575 nm).
表达宿主：HEK293 Cells
蛋白活性：1.Flow cytometric analysis of anti-c-Kit CAR expression. 5e5 of anti-c-Kit CAR-293 cells were stained with 100 μL of 1:100 dilution (1 μL stock solution in 100 μL FACS buffer) of PE-conjugated human c-Kit Protein (Site-Specific PE Conjugation, ECD, His Tag, Cat. No. 11996-H86H-SP) and negative control protein. Non-transduced 293 cells were used as a control (left). PE signal was used to evaluate the binding activity (QC tested). 2.Binding activity of PE-conjugated c-Kit protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and PE-conjugated human c-Kit Protein (Site-Specific PE Conjugation, ECD, His Tag, Cat. No. 11996-H86H-SP) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. PE signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Gln 26
蛋白分子量：The protein has a predicted molecular mass of 58.6 kDa.
蛋白NP号：P10721-2
蛋白氨基酸序列：Met1-Thr516