BioSIM 人 anti-PD-L1 x TGF-β tr

BioSIM 人 anti-PD-L1 x TGF-β trap 融合蛋白 (必特芙普α)，生物类似药，科研用途

CAS号：1918149-01-5

产品核心基础信息

• 英文全称：BioSIM Human anti-PD-L1 x TGF-β trap fusion protein (Bintrafusp Alfa Biosimilar), Research Grade
• 供应商：InvivoCrown
• 产品货号：SIM0614-1mg
• 单份规格：1mg
• 产品类型：重组融合蛋白

生产工艺与背景

本重组生物类似药遵循无动物源生产体系，全程采用体外无蛋白细胞培养技术，从生产环境到原料均避免动物源性成分引入，降低交叉污染风险。

产品经多步纯化工艺精制，核心步骤包含蛋白A/G亲和色谱，配合后续精制工序，确保目标蛋白纯度与活性满足科研级实验要求。

表达系统与缓冲液参数

表达系统

重组蛋白由哺乳动物细胞表达系统生产，可模拟天然蛋白的翻译后修饰（如糖基化等），更贴近体内生理状态，适合免疫相关机制研究。

缓冲液组成

• 基础缓冲液：PBS（磷酸盐缓冲液）
• pH值：7.4（生理环境相近）
• 添加剂：不含稳定剂、防腐剂

科研应用方向

本产品聚焦肿瘤免疫领域，适用于以下研究场景：

• 胆管癌的免疫调控机制研究
• 肺癌（含非小细胞肺癌NSCLC）的靶点相互作用研究
• anti-PD-L1与TGF-β trap双功能蛋白的生物学活性验证

保存与稳定性建议

为维持产品活性，需严格遵循储存规范：

• 短期使用（1-2周）：4℃避光保存（避免反复开盖）
• 中期保存（≤12个月）：-20℃避光保存（建议分装为单次使用量）
• 长期保存：-80℃避光保存（最佳长期稳定性条件）
• 关键注意：严禁反复冻融循环，以防蛋白构象改变导致活性下降

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

BioSIM Human anti-PD-L1 x TGF-β trap fusion protein (Bintrafusp Alfa Biosimilar), for Research Use

CAS No.:1918149-01-5

Core Product Information

• Full English Name:BioSIM Human anti-PD-L1 x TGF-β trap fusion protein (Bintrafusp Alfa Biosimilar), Research Grade
• Supplier:InvivoCrown
• Catalog No.:SIM0614-1mg
• Package Size:1mg
• Product Type:Recombinant Fusion Protein

Manufacturing Process and Background

This recombinant biosimilar follows an animal-free production system, using in vitro protein-free cell culture technology throughout the process. From the production environment to raw materials, animal-derived components are avoided to reduce the risk of cross-contamination.

The product is purified through a multi-step purification process, with key steps including Protein A/G affinity chromatography, combined with subsequent refinement procedures to ensure the purity and activity of the target protein meet research-grade experimental requirements.

Expression System and Buffer Parameters

Expression System

The recombinant protein is produced using a mammalian cell expression system, which can simulate post-translational modifications of natural proteins (such as glycosylation), making it closer to in vivo physiological conditions and suitable for immune mechanism-related research.

Buffer Composition

• Base Buffer:PBS (Phosphate Buffered Saline)
• pH Value:7.4 (similar to physiological environment)
• Additives:Free of stabilizers and preservatives

Research Applications

This product focuses on the field of tumor immunity and is suitable for the following research scenarios:

• Research on immunomodulatory mechanisms in cholangiocarcinoma
• Research on target interactions in lung cancer (including non-small cell lung cancer NSCLC)
• Biological activity verification of the bifunctional anti-PD-L1 and TGF-β trap protein

Storage and Stability Recommendations

To maintain product activity, storage guidelines must be strictly followed:

• Short-term use (1-2 weeks):Store at 4°C protected from light (avoid repeated opening)
• Medium-term storage (≤12 months):Store at -20°C protected from light (aliquoting into single-use portions is recommended)
• Long-term storage:Store at -80°C protected from light (optimal condition for long-term stability)
• Key Note:Repeated freeze-thaw cycles are strictly prohibited to prevent conformational changes in the protein leading to decreased activity

Note: The above information may change due to factors such as product optimization and upgrades. Please consult Yaokein Company for specific details.